ANALISIS KADAR GULA TOTAL, FLAVONOID, DAN UJI AKTIVITAS ANTIOKSIDAN DARI FORMULASI BROTOWALI DENGAN APEL MANALAGI SEBAGAI MINUMAN FUNGSIONAL

Abstract

Currently, the highest number of deaths in Indonesia is caused by non-communicable diseases. One of the non-communicable diseases is diabetes mellitus which is a metabolic disease. Efforts to prevent diabetes mellitus with non-pharmacological therapy can use plants that have the potential to have anti-diabetic effects. This study aims to make brotowali with manalagi apples as a functional drink which has potential as an antidiabetic and to determine total sugar content, flavonoids, and antioxidant activity with 3 ratios of brotowali : manalagi apple (F1 1:2, F2 1:1, and F3 2:1). This study used a completely randomized design (CRD) with 3 formulations and 3 repetitions. The statistical tests used were Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT). The results showed that F1, F2, and F3 averaged total sugar content respectively 27.4%, 22.3% and 20.9%, the average of flavonoids was 41.7 mgQE/g, 48.8 mgQE/g, and 62.8 mgQE/g, and the average antioxidant activity with IC50 values were 145.5 µg/ml, 109.3 µg/ml, and 92.2 µg/ml. The best formulation of brotowali functional drink with manalagi apple is formula F3 with total sugar content of 20.9%, flavonoid content of 62.8 mgQE/g and antioxidant activity with IC50 value of 92.2 µg/ml.