Characterization and tyrosinase activities of a mixture of β-sitosterol and stigmasterol from Bauhinia rufescens Lam

Aminu Muhammad; Musa Maikudi Idris; Umar Ali; Abdulganiyu Umar; Hasnah Mohd Sirat

doi:10.20884/1.api.2023.11.2.6284

Aminu Muhammad Department of Chemistry, Faculty of Science, Yusuf Maitama Sule University, Kano State, Nigeria http://orcid.org/0000-0002-5193-1687
Musa Maikudi Idris Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University Kano, Kano State, Nigeria
Umar Ali Department of Chemistry, Faculty of Natural and Applied Sciences, Kafin Hausa, Jigawa State, Nigeria
Abdulganiyu Umar Department of Chemistry, Faculty of Science, Yusuf Maitama Sule University, Kano State, Nigeria http://orcid.org/0000-0001-7479-6695
Hasnah Mohd Sirat Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.

DOI: https://doi.org/10.20884/1.api.2023.11.2.6284

Abstract

Background: Phytosterols are steroid compounds present in plants that are similar to cholesterol in structure and functions. In this work, stem bark of Bauhinia rufescens was extracted with petroleum ether.

Objective: This work aims to determine anti-tyrosinase activities of a mixture of β-sitosterol and stigmasterol from the stem bark B, rufescens for the treatment of dermatological disorders.

Methods: This work is presenting isolation, characterization and anti-tyrosinase activities of a mixture of β-sitosterol and stigmasterol from the stem bark B. rufescens. The extract was subjected to chromatographic techniques to afford a phytosterol identified as a mixture of β-sitosterol and stigmasterol based on GC-MS, IR, ¹HNMR and ¹³CNMR data.

Results: The phytosterol isolated showed inhibition of tyrosinase activity at 57.1%±0.03 as compared to kojic acid with 85.0% ± 0.01 (sample concentrations of 0.1 mg/mL).

Conclusion: The finding of this study revealed that, the mixture of β-sitosterol and stigmasterol has potential to be developed as tyrosinase inhibitors for the treatment of dermatological disorders.