Sesquiterpenoids from The Stem Bark of Aglaia cucullata (Meliaceae) and Their Cytotoxic Activity Against A549 Lung Cancer Cell Lines

  • Iqbal Wahyu Mustaqim Universitas Padjadjaran
  • Desi Harneti Departement of Chemistry Universitas Padjadjaran
  • Al Arofatus Naini Universitas Padjadjaran
  • Erina Hilmayanti Universitas Padjadjaran
  • Darwati Darwati Universitas Padjadjaran
  • Nurlelasari Nurlelasari Universitas Padjadjaran
  • Tri Mayanti Universitas Padjadjaran
  • Rani Maharani Universitas Padjadjaran
  • Kindi Farabi Universitas Padjadjaran
  • Ace Tatang Hidayat Universitas Padjadjaran
  • Unang Supratman Universitas Padjadjaran
  • Sofa Fajriah 3Research Center for Raw Materials for Medicine and Traditional Medicine, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten 15314, Indonesia
  • Mohamad Nurul Azmi 4School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Yoshihito Shiono 5Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan

Abstract

ABSTRACT. Sesquiterpenoids are a class of terpenoid compounds with a remarkable diversity of structures and biological activities. Sesquiterpenoids are primarily found in higher plants, such as the Meliaceae family's Aglaia genus. Aglaia cucullata is a species of Aglaia that is still rarely explored and can potentially contain sesquiterpenoid compounds with cytotoxic activity. Hence, the research intended to isolate sesquiterpenoids from the n-hexane extract of A. cucullata stem bark and evaluate their cytotoxic effect against A549 lung cancer cells. Compounds 1 and 2 were isolated and purified from n-hexane extracts utilizing various chromatographic techniques. The structure of compounds 1 and 2 was determined by analyzing various spectroscopic methods (IR, MS, and NMR) and comparing them to previously reported spectral data. Compound 1 was identified as an isodaucane-type sesquiterpenoid, 10-hydroxy-6,10-epoxy-7(14)-isodaucane, and was first reported in Meliaceae family. Compound 2 was confirmed as an eudesmane-type sesquiterpenoid, eudesm-4(15)-ene-1β,6α-dihydroxy, and was first reported in Aglaia cucullata. Cytotoxic activity of 1 and 2 were investigated in vitro against A549 lung cancer cells using the PrestoBlue assay and resulted in inactive and low cytotoxicity with IC50 values of 292.77 and 90.55 μM, respectively.


 


Key words: Aglaia cucullata, , A549 cell lines, cytotoxic activity, Meliaceae, sesquiterpenoids.


 

Author Biographies

Iqbal Wahyu Mustaqim, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Al Arofatus Naini, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Erina Hilmayanti, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Darwati Darwati, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Nurlelasari Nurlelasari, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Tri Mayanti, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Rani Maharani, Universitas Padjadjaran

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Kindi Farabi, Universitas Padjadjaran

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

2Central Laboratory, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Ace Tatang Hidayat, Universitas Padjadjaran

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Unang Supratman, Universitas Padjadjaran

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

2Central Laboratory, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Sofa Fajriah, 3Research Center for Raw Materials for Medicine and Traditional Medicine, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten 15314, Indonesia

3Research Center for Raw Materials for Medicine and Traditional Medicine, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten 15314, Indonesia

Mohamad Nurul Azmi, 4School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

4School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

Yoshihito Shiono, 5Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan

5Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan

Published
2024-07-18
How to Cite
MUSTAQIM, Iqbal Wahyu et al. Sesquiterpenoids from The Stem Bark of Aglaia cucullata (Meliaceae) and Their Cytotoxic Activity Against A549 Lung Cancer Cell Lines. Molekul, [S.l.], v. 19, n. 2, p. 242-249, july 2024. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/9823>. Date accessed: 29 mar. 2025. doi: https://doi.org/10.20884/1.jm.2024.19.2.9823.
Section
Articles

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