Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae

  • Indah Sulistiyawati Biological Science Doctoral Program, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia
  • Daniel Joko Wahyono Department of Molecular Biology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia
  • Wahyu Siswandari Department of Clinical Pathology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Indonesia
DOI: https://doi.org/10.20884/1.jm.2024.19.1.10134

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively.


 


Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability

Published
2024-03-14
How to Cite
SULISTIYAWATI, Indah; WAHYONO, Daniel Joko; SISWANDARI, Wahyu. Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae. Molekul, [S.l.], v. 19, n. 1, p. 172-179, mar. 2024. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/10134>. Date accessed: 20 sep. 2024. doi: https://doi.org/10.20884/1.jm.2024.19.1.10134.
Citation Formats
Issue
Vol 19 No 1 (2024)
Section
Articles

Authors agree with the statements below:

  1. Authors automatically transfer the copyright to the MOLEKUL journal and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
  2. Authors are able to enter into separate permission for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.