INDIGENOUS FUNGI FROM MARIBAYA-BREBES-BUMIAYU: POTENTIAL FOR BATIK DYE WASTE BIODEGRADATION

Ratna Stia Dewi; Dyahruri Sanjayasari; Mardiyah Kurniasih; Any Kurniawati; Wilda Khafida; Firni Hasna Syifaunnisa; Eka Widya Ningsih; Quina Alifa

Ratna Stia Dewi Department of Microbiology Program, Faculty of Biology, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Dyahruri Sanjayasari Department of Aquatic Resources Management Undergraduate, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Mardiyah Kurniasih Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Any Kurniawati Department of Aquatic Resources Management Undergraduate, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Wilda Khafida Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Firni Hasna Syifaunnisa Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman
Eka Widya Ningsih Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman
Quina Alifa Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman; Center for Biodiversity and Maritime Research, Institute for Research and Community Service, Universitas Jenderal Soedirman

Abstract

Batik dye waste is a source of pollution that is difficult to degrade naturally and requires an effective biological approach. This study aims to evaluate the ability of four indigenous fungal isolates from Maribaya–Brebes–Bumiayu (Fusarium sp., Aspergillus sp., Trichoderma sp., and Rhizopus sp.,) to grow on PDA media mixed with batik waste. Each isolate was tested at three waste concentration treatments, namely 0%, 5%, and 20%. The results showed that all isolates were able to grow stably at all treatments, indicating a good level of tolerance to toxic components in batik waste. Aspergillus sp. showed the strongest and most consistent growth at all waste concentrations, making it the leading candidate for bioremediation applications. Meanwhile, Fusarium sp., Trichoderma sp., and Rhizopus sp. still showed good adaptation potential and are relevant for further study. These findings confirm that indigenous fungi from Maribaya have great potential as agents for the biodegradation of batik waste and can support the development of waste treatment technology based on local resources.