Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae

  • Indah Sulistiyawati Biological Science Doctoral Program, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia
  • Daniel Joko Wahyono Department of Molecular Biology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia
  • Wahyu Siswandari Department of Clinical Pathology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Indonesia

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively.


 


Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability

Published
2024-03-14
How to Cite
SULISTIYAWATI, Indah; WAHYONO, Daniel Joko; SISWANDARI, Wahyu. Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae. Molekul, [S.l.], v. 19, n. 1, p. 172-179, mar. 2024. ISSN 2503-0310. Available at: <http://jos.unsoed.ac.id/index.php/jm/article/view/10134>. Date accessed: 17 apr. 2024. doi: https://doi.org/10.20884/1.jm.2024.19.1.10134.
Section
Articles