Phytochemical screening and purification of n-hexane fraction of Calophyllum soulattri leaves

  • Sunarto Sunarto Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia)
  • Agnes Yuliasari Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia)
  • Sri Sutji Susilowati Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia)
  • Hendri Wasito Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia) http://orcid.org/0000-0001-7176-9894
  • Triyadi Hendra Wijaya Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia) http://orcid.org/0000-0003-0666-7757
  • Muhamad Salman Fareza Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia) http://orcid.org/0000-0003-2414-1249

Abstract

BackgroundCalophyllum soulattri Burm F. is widely utilized in traditional medicine. It is necessary to identify secondary metabolites from C. soulattri leaves to determine the pharmacologically active chemicals.


Objective: This study aimed to screen the phytochemical content and purify the n-hexane fraction of C. soulattri leaves from Banyumas, Indonesia.


Methods: The n-hexane fraction was macerated with methanol, followed by liquid-liquid fractionation with n-hexane. The n-hexane fraction was tested for flavonoids, triterpenoids/steroids, saponins, and phenols using the test tube method. In addition, the compounds were purified using column chromatography. The purified compound was identified by the Liebermann-Burchard reagent, which was compared with commercially available steroid drugs as reference. 


Results: Phytochemical analysis revealed that the n-hexane fraction of C. soulattri leaves contained secondary metabolites such as flavonoid, steroid, and phenol compounds. Analyses with the Liebermann-Burchard reagent indicated that the purified compound was potentially a steroid.


Conclusion: The compound extracted from the n-hexane fraction of C. soulattri leaves was expected as a steroid.


 

Published
2022-08-01
How to Cite
SUNARTO, Sunarto et al. Phytochemical screening and purification of n-hexane fraction of Calophyllum soulattri leaves. Acta Pharmaciae Indonesia : Acta Pharm Indo, [S.l.], v. 10, n. 2, p. 5858, aug. 2022. ISSN 2621-4520. Available at: <http://jos.unsoed.ac.id/index.php/api/article/view/5858>. Date accessed: 03 dec. 2022. doi: https://doi.org/10.20884/1.api.2022.10.2.5858.