THE EFFECT OF ETHANOL EXTRACT OF Rhizophora mucronate LEAVES ON THE GROWTH OF Staphylococcus aureus BACTERIA

Abstrak

Staphylococcus aureus bacteria which are opportunistic pathogens and are responsible for the problem of Methicillin and Vancomycin antibiotic resistance against S.aureus. Mangrove leaves R. mucronata contain antibacterial activity in secondary metabolite compounds, there are phenols, flavonoids, saponins, tannins, and terpenoids. The purpose of this study was to determine the effect of 96% ethanol extract of mangrove leaves R. mucronata on the growth of Staphylococcus aureus bacteria. The study was conducted experimentally in a laboratory with Staphylococcus aureus as a sample. Staphylococcus aureus used random sampling techniques, mixed with distilled water and its turbidity equivalent to 0.5 Mc Farland standardization, planted on Mueller Hinton agar. Mangrove leaves R. mucronata were extracted using the maceration method and 4 series of concentrations were made (25%, 50%, 75%, and 100%). The antibacterial test used the disc diffusion method with a positive control of 5 mcg Ciprofloxacin and a negative control of sterile distilled water. All petri dishes were put into an incubator for 24 hours at 37°C, then their diameters were measured. Data were analyzed statistically using the Kruskal Wallis Nonparametric test and continued with the Mann Whitney-U posthoc test. The results show an increase in the diameter of the inhibition zone for each extract concentration with the highest inhibitory activity at a concentration of 100% of 11.7 mm. The conclusion in this study is that 96% ethanol extract of R. mucronata mangrove leaves can inhibit the growth of S. aureus bacteria in vitro