Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera

  • Ni Putu Kasturiasih Hasanuddin University
  • Ahyar Ahmad Hasanuddin University
  • Rugaiyah A. Arfah Hasanuddin University
  • Siti Khairunnur Hasanuddin University
  • Nunuk Hariani Soekamto Hasanuddin University
  • Paulina Taba Hasanuddin University
  • Yusafir Hala Hasanuddin University
  • Harningsih Karim Department of Pharmacy, School of Pharmacy Yayasan Ma’bullo Sibatang
DOI: https://doi.org/10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.

Author Biographies

Ahyar Ahmad, Hasanuddin University

Chemistry Department, Mathematics and Natural Sciences Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia.

Professor of Biochemistry, Hasanuddin University.

 

Rugaiyah A. Arfah, Hasanuddin University

Chemistry Department, Mathematics and Natural Sciences Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia.

Lecturer of Biochemistry, Hasanuddin University.

Siti Khairunnur, Hasanuddin University

Master Program, Chemistry Department, Mathematics and Natural Sciences Faculty, Biochemistry Lab. Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia.

Student of  Chemistry Master Program.

Published
2023-11-20
How to Cite
KASTURIASIH, Ni Putu et al. Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera. Molekul, [S.l.], v. 18, n. 3, p. 442-449, nov. 2023. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/8153>. Date accessed: 14 jan. 2025. doi: https://doi.org/10.20884/1.jm.2023.18.3.8153.
Citation Formats
Issue
Vol 18 No 3 (2023)
Section
Articles

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