A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

  • Mashuni Mashuni Halu Oleo University
  • Nur Arfa Yanti Halu Oleo University
  • Irnawati Irnawati Halu Oleo University
  • Fitri Handayani Hamid Halu Oleo University
  • Muhammad Jahiding Halu Oleo University

Abstract

Long-term accumulation of pesticides in the environment  to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.

Author Biographies

Mashuni Mashuni, Halu Oleo University

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

Nur Arfa Yanti, Halu Oleo University

Department of Biology, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

Fitri Handayani Hamid, Halu Oleo University

Magister of Chemistry, Program of Postgraduate, Halu Oleo University, Kendari, 93132, Indonesia

Muhammad Jahiding, Halu Oleo University

Department of Physics, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

Published
2023-03-20
How to Cite
MASHUNI, Mashuni et al. A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection. Molekul, [S.l.], v. 18, n. 1, p. 131-139, mar. 2023. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/6864>. Date accessed: 19 july 2024. doi: https://doi.org/10.20884/1.jm.2023.18.1.6864.
Section
Articles