Molecularly Imprinted Polymer Solid-Phase Extraction (MISPE) for the Determination of Dinitro-ortho-cresol (DNOC) and Its Risk Assessment in Tomatoes

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  • Raden Tina Rosmalina Research Centre for Environmental and Clean Technology, BRIN
  • Muhammad Bachri Amran Analytical Chemistry Division, Institut Teknologi Bandung, Indonesia
  • Bimastyaji Surya Ramadan Diponegoro University, Indonesia
  • Sri Priatni 1Research Centre for Environmental and Clean Technology, National Research and Innovation Agency, Indonesia
  • Novi Fitria Higher School of Analyst Bakti Asih Bandung, Indonesia

Abstract

In this study, the molecularly imprinted polymer solid-phase extraction (MISPE) method for the determination of dinitro-ortho-cresol (DNOC) before HPLC (High-performance liquid chromatography) analysis was conducted. This study aims to analyze the sorption behavior and the potential use of molecularly imprinted polymers (MIP) for DNOC extraction. MIP was prepared using a combination of methacrylic acid as the functional monomer; ethylene glycol dimethacrylate as the crosslinker, benzoyl peroxide as the initiator, and acetonitrile as the porogen. The results showed that the optimum adsorption of the material was achieved at pH 5 for a contact time of 30 min with an adsorption capacity of 137 mg/g. The ideal eluent for desorption was a mixture of methanol and acetic acid with a ratio of 3:1. The calculations showed that the adsorption process followed the Sips adsorption isotherm model (n = 0.967), which indicated adsorption on both homogeneous and monolayer surfaces. Reusability studies that were conducted via three consecutive adsorption-desorption cycles resulted in recovery values of 109.4%, 108.8%, and 101.1%. The concentration of DNOC obtained from tomato samples was 0.65 mg/kg with a recovery rate of 87.17%; this was within the tolerable range between 80% and 110%. Based on the high recovery and low LoQ, this method can be used to quantify DNOC quickly.

Author Biographies

Muhammad Bachri Amran, Analytical Chemistry Division, Institut Teknologi Bandung, Indonesia

His Department is Chemistry Department in ITB. He is a senior lecturer as a profesor ih his field. His filed is analytical chemistry, separation, adsorption etc

Bimastyaji Surya Ramadan, Diponegoro University, Indonesia

Bimastyaji Surya Ramadan is a junior lecturer and researcher from Department of Environmental Engineering, Universitas Diponegoro, Indonesia. At the time of submission of this article, he was pursuing a doctoral degree in The University of Kitakyushu, Japan. He focuses on waste management system, air pollution control and treatment, and environmental remediation. He published many articles in reputable journal and chapters.

Sri Priatni, 1Research Centre for Environmental and Clean Technology, National Research and Innovation Agency, Indonesia

She is an expert in food and biotechnology.

Novi Fitria, Higher School of Analyst Bakti Asih Bandung, Indonesia

She is an expert in environmental engineering and risk assesment.

Published
2023-03-20
How to Cite
ROSMALINA, Raden Tina et al. Molecularly Imprinted Polymer Solid-Phase Extraction (MISPE) for the Determination of Dinitro-ortho-cresol (DNOC) and Its Risk Assessment in Tomatoes. Molekul, [S.l.], v. 18, n. 1, p. 29-40, mar. 2023. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/5852>. Date accessed: 18 may 2024. doi: https://doi.org/10.20884/1.jm.2023.18.1.5852.
Section
Articles