Synthesis and Antibacterial Activity of N-phenyl-3-(4-chlorophenyl)-5-(3,4-dimethoxy-phenyl) pyrazoline

  • Estin Nofiyanti Environmental Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia
  • Tutik Dwi Wahyuningsih Chemical Organic Laboratory, Chemistry Department, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Noer Laelly BTAG Electronichal Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia
  • Chairil Anwar Chemical Organic Laboratory, Chemistry Department, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Novi Nurjanah Environmental Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia
  • Nurcholis Salman Environmental Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia
  • Yoga Priastomo Chemical Organic Laboratory, Chemistry Department, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
DOI: https://doi.org/10.20884/1.jm.2025.20.2.5618

Abstract

ABSTRACT. Compound of N-phenyl-3-(4-chlorophenyl)-5-(3,4-dimethoxy-phenyl) pyrazoline was synthesized by cycloaddition reaction. The structure of the product was chracterized by FTIR,  1H-NMR and 13C-NMR spectrometers.  The pyrazoline was screened for antibacterial activity using agar diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtillis, Shigella flexnerri, and Escherichia coli. The pyrazoline had been synthesized from chalcone with phenylhydrazine to give 52.90% yield. Antibacterial screening showed that pyrazoline was active against selected gram positive bacteria. The pyrazoline was found to exhibits an antibacterial activity and its zone of inhibition/concentration against S. aureus (8.75/300), B. cereus (7.75/1000), B. subtillis (6.50/1000), and E. coli (3.50/300). The result showed that pyrazoline which was subtituted with chloro and methoxy, was able to posse broad spectrum of the tested pathogenic bacteria.


 


Keywords: chalcone, pyrazoline, antibacterial

Published
2025-07-24
How to Cite
NOFIYANTI, Estin et al. Synthesis and Antibacterial Activity of N-phenyl-3-(4-chlorophenyl)-5-(3,4-dimethoxy-phenyl) pyrazoline. Molekul, [S.l.], v. 20, n. 2, p. 197-202, july 2025. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/5618>. Date accessed: 20 aug. 2025. doi: https://doi.org/10.20884/1.jm.2025.20.2.5618.
Citation Formats
Issue
Vol 20 No 2 (2025)
Section
Articles

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