Antioxidant and Antibacterial Activities of Plant and Endophytic Fungi Extracts from Syzygium myrtifolium Walp, with LC-HRMS Profiling of Active Extracts

  • Sintia Frisky Efendi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University. Padang, West Sumatra, Indonesia
  • Suryati Suryati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University. Padang, West Sumatra, Indonesia
  • Dwinna Rahmi Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN), KST Soekarno, Cibinong, West Java, Indonesia.
  • Praptiwi Praptiwi Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN), KST Soekarno, Cibinong, West Java, Indonesia.
  • Muhammad Ilyas Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), KST Soekarno, Cibinong, West Java, Indonesia.
  • Andria Agusta Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN), KST Soekarno, Cibinong, West Java, Indonesia.
DOI: https://doi.org/10.20884/1.jm.2025.20.2.16366

Abstract

ABSTRACT. The emergence of antibiotic resistance and oxidative stress-related diseases highlights the urgent need for novel bioactive compounds. This study investigates the potential of Syzygium myrtifolium Walp. and its endophytic fungi as sources of antibacterial and antioxidant agents. Sixteen endophytic fungi isolates were obtained from six plant parts and identified morphologically. Thin layer chromatography (TLC)-based chemical profiling demonstrated comparable secondary metabolite patterns between the plant and its endophytic fungi, indicating possible shared biosynthetic pathways. Antibacterial screening using TLC-bioautography and minimum inhibitory concentration (MIC) assays demonstrated that both the plant and endophytic fungi extracts inhibited the growth of Staphylococcus aureus and Escherichia coli, with Xylaria sp. showing notable activity. (SmDh4) exhibiting the strongest activity (MIC = 64 µg/mL). Antioxidant activity determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay indicated high to very high radical inhibition capacity, especially in SmRTd (AAI = 25.91) and SmAk1 (AAI = 24.97). Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis unique secondary metabolites on endophytic fungi, including L-α-palmitin, α-eleostearic acid, and 8-methylnaphthalene-1,2-diol, which were detected exclusively in the endophytic fungi extracts, highlighting their potential as alternative antibacterial and antioxidant agents.


 


Keywords: Antibacterial, antioxidant, endophytic fungi, secondary metabolites, Syzygium myrtifolium Walp.

Published
2025-07-24
How to Cite
EFENDI, Sintia Frisky et al. Antioxidant and Antibacterial Activities of Plant and Endophytic Fungi Extracts from Syzygium myrtifolium Walp, with LC-HRMS Profiling of Active Extracts. Molekul, [S.l.], v. 20, n. 2, p. 404-414, july 2025. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/16366>. Date accessed: 20 aug. 2025. doi: https://doi.org/10.20884/1.jm.2025.20.2.16366.
Citation Formats
Issue
Vol 20 No 2 (2025)
Section
Biomolecule Isolation, Modification and Application

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