Development and Validation of Zerumbone Analysis Method Using Ultraviolet-Visible Spectrophotometry

  • Haeria Doloking Pharmacy Department, Faculty of Medical and Health Sciences, Universitas Islam Negeri Alauddin Makassar, Gowa, Sulawesi Selatan, 92118, Indonesia.
  • Muhlisuddin Muhlisuddin Pharmacy Department, Faculty of Medical and Health Sciences, Universitas Islam Negeri Alauddin Makassar, Gowa, Sulawesi Selatan, 92118, Indonesia.
  • Nurul Muhlisah Maddeppungeng Pharmacy Department, Faculty of Medical and Health Sciences, Universitas Islam Negeri Alauddin Makassar, Gowa, Sulawesi Selatan, 92118, Indonesia.
DOI: https://doi.org/10.20884/1.jm.2025.20.3.15690

Abstract

ABSTRACT. Analytical method development ensures that an analytical procedure is suitable for the identification and quantification of compounds of interest. This study aimed to develop and validate a simple, cost-effective analytical method for the determination of zerumbone using UV-Vis spectrophotometry. Method validation was conducted according to ICH Q2(R2) guidelines, including tests for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision. Zerumbone exhibited a maximum absorption wavelength (λmax) of 212 nm when dissolved in dimethyl sulfoxide (DMSO). The method demonstrated excellent linearity with a determination coefficient (R2) of 0.9991 across a concentration range of 10-50 μg/mL. Accuracy, evaluated via recovery studies, ranged from 91,00% to 110,45%. Precision testing results in a % RSD of 0.58%. The calculated LOD and LOQ were 1.55 μg/mL and 4.70 μg/mL, respectively. This validated method is simple, rapid, and economical, making it a promising alternative for zerumbone analysis in quality control laboratories.


 


Keywords: Zerumbone, Method Development, UV-Vis Spectrophotometry, Validation, ICH Guidelines.


 

Published
2025-11-24
How to Cite
DOLOKING, Haeria; MUHLISUDDIN, Muhlisuddin; MADDEPPUNGENG, Nurul Muhlisah. Development and Validation of Zerumbone Analysis Method Using Ultraviolet-Visible Spectrophotometry. Molekul, [S.l.], v. 20, n. 3, p. 505-510, nov. 2025. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/15690>. Date accessed: 26 nov. 2025. doi: https://doi.org/10.20884/1.jm.2025.20.3.15690.
Citation Formats
Issue
Vol 20 No 3 (2025)
Section
Analytical Method Development and Validation

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