Silver Nanoparticle Biosynthesis using Distimake vitifolius Extract for Enhancement of Antibacterial and Antioxidant Activity

  • Candra Yulius Tahya Chemistry Education Study Program, Faculty of Education, Pelita Harapan University, Tangerang
  • Karnelasatri Karnelasatri Pharmacy Study Program, Faculty of Health Sciences, Pelita Harapan University, Tangerang
  • Wahyu Irawati Biology Education Study Program, Faculty of Education, Pelita Harapan University, Tangerang
  • Sri Wahyu Ningsih Munthe Pharmacy Study Program, Faculty of Health Sciences, Pelita Harapan University, Tangerang

Abstract

ABSTRACT. The biological method for silver nanoparticle synthesis emphasizes green synthesis, which is faster, environmentally safer, facile, energy-efficient, and cost-effective. It provides good alternatives to physical and chemical methods. In this research, we have synthesized silver nanoparticles using D. vitifolius ethanolic extract. The 38 natural compounds of flavonoid, phenolic or polyphenol compositions have been determined by LC-MS/MS-QTOF which is carried out using UNIFI software equipped with a mass spectrum library of active substances from natural ingredients from the Waters database. Silver nanoparticle (Dv-AgNP) was characterized by using TEM and SEM where the average diameter of nanoparticle was seen <100 nm and confirmed by PSA analysis that the Z-average is 62.73 ± 0.71 nm. EDS spectrum confirmed the silver element as a major element of the particles. PI value of Dv-AgNP is 0.557, while the zeta potential is -17.37 ± 1.53 mV. Dv-AgNP can inhibit the growth of P. aeruginosa (amoxicillin resistant) with an inhibition zone of 15.83 ± 2.36 mm, and K. pneumoniae with an inhibition zone is 10.03 ± 0.55 mm. These are moderate inhibitory activities. The antioxidant result of the Dv-AgNP suspension solution shows that the IC50 is 0.58 mM. The IC50 of the crude extract is 752 ppm and is categorized as very weak antioxidant activity.


 


Keywords: Antibacterial; D. vitifolius; silver nanoparticle.

Published
2024-07-18
How to Cite
TAHYA, Candra Yulius et al. Silver Nanoparticle Biosynthesis using Distimake vitifolius Extract for Enhancement of Antibacterial and Antioxidant Activity. Molekul, [S.l.], v. 19, n. 2, p. 281-295, july 2024. ISSN 2503-0310. Available at: <https://jos.unsoed.ac.id/index.php/jm/article/view/10295>. Date accessed: 27 mar. 2025. doi: https://doi.org/10.20884/1.jm.2024.19.2.10295.
Section
Articles

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