PREPARATION, CHARACTERIZATION, AND TOXICITY STUDY OF Channa striata EXTRACT ALGINATE NANOPARTICLES ON HepG2 CELL

Abstract

Channa striata extract contains albumin protein and high-fatty acids that can accelerate wound healing so it is widely used in health care. Due to the presence of these compounds in Channa striata extract, it faces challenges in penetrating the cell membrane. Particle size reduction can solve the problem, therefore Channa striata extract is formulated within a nanoparticle system combined with biopolymer, such as alginate. The study aims to synthesize Channa striata extract nanoparticles using alginate, characterize the nanoparticle, and evaluate their cytotoxic effects on HepG2 cells line. The Channa striata extract nanoparticle was prepared using ionic gelation, employing different concentrations of alginate and CaCl2 as the counter ion. The characterization of nanoparticles including morphology, particle size, particle size distribution, zeta potential, functional group, and thermal analysis. Channa striata extract nanoparticles using alginate had an irregular morphology with 210.2 nm particle size, PDI of 0.143, and zeta potential of -47.1 mV. The results of functional group and thermal analysis showed that Channa striata extract has been adsorbed in nanoparticle system. The toxicity assay results indicate that alginate nanoparticles of Channa striata extract exhibit no cytotoxic effects on HepG2 cells, as demonstrated by cell viability percentages exceeding 50%.