THE APPLICATION OF ARTIFICIAL INSEMINATION USING SEXED SEMEN ON THE REPRODUCTIVE PERFORMANCE OF SAKUB SHEEP

Hilmy Abdurrasyid Ammar; Mas Yedi Sumaryadi; Agustina Setyaningrum; Dadang Mulyadi Saleh; Aras Prasetyo Nugroho; Chomsiatun Nurul Hidayah

doi:10.20884/2.procicma.2025.1.1.20774

Hilmy Abdurrasyid Ammar Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto
Mas Yedi Sumaryadi Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto
Agustina Setyaningrum Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto
Dadang Mulyadi Saleh Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto
Aras Prasetyo Nugroho Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto
Chomsiatun Nurul Hidayah Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto

DOI: https://doi.org/10.20884/2.procicma.2025.1.1.20774

Abstract

The study aimed to evaluate the reproductive performance of Sakub ewes artificially inseminated using sexed semen from Sakub rams to preserve local genetic resources. Fresh semen was collected from Sakub rams, and analyzed for quality and morphometry. The experiment involved 20 Sakub ewes and 2 Sakub rams. All ewes were synchronized by intramuscular injection of 1 ml prostaglandin (PGF2α) in double doses at 11-day intervals. The research used two treatment groups: group I (10 ewes inseminated using the sexed semen) and group II (10 ewes inseminated using unsexed semen as a control). The proportion of Y spermatozoa in sexed semen was significantly (P<0.01) higher than in unsexed semen, while the quality of sexed semen was significantly lower (P<0.05). The morphometric proportion of Y spermatozoa in sexed and unsexed semen was 71.11 ± 3.27% and 49.58 ± 1.08%, respectively. Motility, viability, abnormality, and spermatozoa concentration in sexed semen were 74 ± 7.75%; 76.29 ± 8.18%; 8.87 ± 3.73% and 5.44 ± 0.50 million/ml, respectively; while in unsexed semen were 76 ± 6.15%; 79 ± 6.35%; 10.75 ± 3.19%; and 5.87 ± 0.65 million/ml, respectively. Both groups exhibited 100% estrus, with estrus intensity scores of 3.25 ± 0.49 and 2.91 ± 0.67. Conception rates were 80 and 90%, respectively. It was concluded that sexing did not affect reproductive performance; however, further research on the offspring sex ratio is needed.