REDUCED MOTILITY, FERTILITY, AND FERTILE PERIOD OF KAMPUNG ROOSTER SPERM FOLLOWING ROOM-TEMPERATURE STORAGE IN A SKIM MILK–GLUCOSE EXTENDER

Abstract

Artificial insemination (AI) is a vital reproductive technology for improving poultry fertility and productivity. However, semen quality deteriorates rapidly at tropical ambient temperatures. This study investigated the effects of short-term room-temperature storage in a skim milk–glucose extender on motility, fertility, and fertile period of Kampung rooster sperm. Semen was collected from ten 15-month-old roosters and inseminated into thirty hens following three storage durations: P₀ = 0–10 min, P₁ = 30–40 min, and P₂ = 60–70 min. Sperm motility (%) remained similar between P₀ (85.0 ± 0.0) and P₁ (79.0 ± 3.33) but declined sharply after 60 min at P₂ (60.0 ± 3.33; P < 0.05). Fertility (%) showed a comparable pattern—no difference between P₀ (82.25 ± 8.12) and P₁ (76.42 ± 7.63), yet a significant reduction at P₂ (60.25 ± 9.61; P < 0.05). The fertile period was shortened only after 60 min of storage. These results indicate that rooster semen can be safely used within 40 min at room temperature, but longer exposure markedly reduces fertility potential.