FORMULATIONS OF LIQUID CARRIER MEDIA BASED ON NATURAL INGREDIENTS FOR HYDROLYTIC AND NITRIFYING BACTERIAL CONSORTIUM OF ORGANIC WASTE BIOREMEDIATION AGENT

Abstract

The study aimed to develop a liquid carrier medium formulation composed of natural materials to support the growth and viability of hydrolytic and nitrifying bacterial consortia for organic waste bioremediation. The bacterial consortium consisted of 5 hydrolytic and 4 nitrifying isolates. The growth of the five hydrolytic bacterial isolates in growth media M1, M2, M3, M4, and M5 during the 30-day incubation period ranged between 1.03x109 – 5.5x109 CFU/mL, while the growth of the four nitrifying bacterial isolates in the five types of growth media ranged between 4.62x107 – 2.91x109 CFU/mL. The growth media M2, M1, and M3 resulted in an average total population of bacterial isolates of 2.53x109, 2.41x109, and 2.29x109 CFU/mL, respectively. The total population of hydrolytic and nitrifying consortia in selected liquid carrier media (M1, M2, M3) decreased with increasing incubation time until 56 days. A significant decrease was found in the control of LB medium and liquid carrier medium M2, from 7.78 log CFU/mL to 5.11 log CFU/mL, and 8.20 log CFU/mL to 5.85 log CFU/mL, respectively. The total bacterial population in M3 and M1 on the 56th day of incubation showed a smaller decrease, from 7.23 and 7.91 log CFU/mL to 6.27 l and 6.58 log CFU/mL, respectively. The use of natural ingredients, such as banana stem and coconut water, in the M2 and M3 media was shown to support the viability of hydrolytic and nitrifying bacteria for 56 days of incubation.