FROZEN GRANULATION OF ETHANOL EXTRACT OF BROWN OYSTER MUSHROOM (Pleurotus cystidiosus): MYCOCHEMICAL PROFILE BASED ON FOURIER TRANSFORM INFRARED (FTIR) AND ANTIBACTERIAL ACTIVITY

Nuniek Ina Ratnaningtyas; Fajar Husen; Nur Fitrianto; Juni Safitri Muljowati

Nuniek Ina Ratnaningtyas Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Fajar Husen Department of Medical Laboratory Technology, Sekolah Tinggi Ilmu Kesehatan Bina Cipta Husada Purwokerto, Purwokerto, Indonesia
Nur Fitrianto Research Center for Food Technology and Processing, Research Organization for Agriculture and Food, National Research and Innovation Agency, Yogyakarta, Indonesia
Juni Safitri Muljowati Department of Biology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia

Abstract

Brown oyster mushroom (Pleurotus cystidiosus) is a group of edible mushrooms found in many tropical countries. The utilization of P. cystidiosus mushrooms is still limited to daily food applications; even though they have much potential, they are developed into antioxidant, anti-inflammatory, or immunomodulatory supplements. In addition, manufacturing preparations from mushrooms to obtain bioactive compounds is still widely done by extraction methods with the principle of maceration. The maceration method takes a long time and wastes solvents, requiring high costs. Based on this, this study was conducted to prepare a frozen granulation of P. cystidiosus using freeze drying. The extraction process was carried out by sonication using a sonicator at a frequency of 50 Hz for 45 minutes for three running cycles, identifying the content of bioactive compounds using Fourier Transform Infrared (FTIR), and testing the activity of P. cystidiosus granules as antibacterial against four pathogenic bacterial species namely Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Pseudomonas aeruginosa. Using the disc method. The research method was descriptive-analytical, where the results of compound identification were characterized and identified with an FTIR instrument. At the same time, antibacterial testing was carried out using the MIC macro-broth dilution method with a complete randomized design (CRD) with further tests using the Duncan-multiple range test using SPSS software ver. 27.0 and GraphPad Prism Ver 10.3.0. The results showed that the frozen granule of P. cystidiosus mushroom contained many diverse compounds and had the potential to be further developed and isolated to P. cystidiosus granules, which can also inhibit the growth of four types of pathogenic microorganisms used in antibacterial testing. The development of P. cystidiosus granule preparation can be continued by encapsulating it using a suitable coating and can be continued to be carried out in vivo testing.