MOTILITY AND FERTILIZING ABILITY OF KAMPUNG ROOSTER SPERM DILUTED WITH SKIM MILK +GLUCOSE+ DMA EXTENDER STORED AT 5oC FOR UP TO 6 HOURS

Abstract

Semen extenders are crucial for maintaining sperm quality during storage and ensuring the success of fertilization after insemination. The aim of this research is to test the effects of a skim milk-based extender for rooster semen supplemented with glucose and DMA on sperm motility and fertilization ability after being stored in a refrigerator at a temperature of 5°C for up to 6 hours. In this study, to observe the quality of spermatozoa and fertility diluted with skim milk + 50 mM Glucose + (0.5 and 10%) DMA at a storage temperature of 5°C and storage duration (0, 3 dan 6 h).The results show that there is no interaction effect from the type of diluent (addition of DMA: (0, 5, and 10%) and storage duration (0, 3, and 6 hours) on the motility and fertility of Kampung rooster spermatozoa.Skim milk extender plus DMA (0, 5, and 10%) was not significant (P>0.05) in improving the quality of rooster semen (motility and fertility) during storage for up to 6 hours. Sperm diluted with skim milk + Glucose + DMA (0.5% and 10%) stored at 5°C for 3 hours resulted in significantly higher motility and fertility values (P<0.01) compared to the motility and fertility values stored for 6 hours. In conclusion, the extender of skim milk + 50 mM glucose and + (5 and 10 percent DMA) stored at 5°C for 3 hours, positively affects the motility and fertility of Kampung rooster spermatozoa.