HYDROLYSIS OF SOYBEAN PROTEIN WITH ANTIOXIDANT ACTIVITY BY FRACTIONATED PROTEASE FROM Bacillus subtilis B298

Puji Lestari; Suyata Suyata; Niken Istikhari Muslihah

Puji Lestari Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Suyata Suyata Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Niken Istikhari Muslihah Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia

Abstract

Research has been carried out to obtain bioactive peptides with antioxidant potential from soybean protein. Bioactive peptides were produced by enzymatic hydrolysis using fractionated protease with the highest specific activity from Bacillus subtilis B298 bacteria. The aim of the research was to determine: the biochemical characteristics of fractionated protease with the highest specific activity, the effect of variations in hydrolysis time on the antioxidant activity of bioactive peptides by protease, the AAI (Antioxidant Activity Index) and the hemolysis percentage of bioactive peptide with the highest antioxidant activity. This research was carried out in stages: extraction and fractionation of the protease enzyme, characterization of the protease fraction with the highest specific activity, hydrolysis of soybean protein with varying incubation times of 10, 20, 30, 40, 50 and 60 minutes, antioxidant activity test, determination of AAI and percentage of hemolysis of bioactive peptide with the highest antioxidant activity. The results showed that the protease fraction with the highest specific activity was F15 with an optimum temperature of 45 °C and an optimum pH of 8. The highest antioxidant activity was obtained from bioactive peptide which was hydrolysed for 10 minutes with an inhibition percentage of 63.1%. The AAI was obtained at 0.119 and the hemolysis percentage was 3%.