CYTOTOXIC TEST OF BIOACTIVE COMPOUNDS FROM FRUIT BODY EXTRACTS OF Pleurotus ostreatus ON BREAST CANCER CELLS
Abstract
The purpose of this study was to identify the classes of bioactive compounds in the n-hexane, chloroform, and ethyl acetate extracts from the fruiting body of the Pleurotus ostreatus mushroom, as well as to evaluate the cytotoxic activity of these extracts against T47D and MCF7 breast cancer cells. The methods employed were both descriptive and experimental. The independent variable in this study was the administration of P. ostreatus extract to cancer cell cultures at various concentrations (1000, 500, 250, 125, 62.50, and 31.25 µg/mL). The MTT assay was used to measure the cytotoxic activity of the mushroom extracts against T47D and MCF7 breast cancer cells. The main parameters analyzed included the percentage of cell viability, the IC50 value, and the types of bioactive compounds based on the KLT test. Data were analyzed using linear regression, while the detection of bioactive compounds was conducted descriptively by observing the eluted color spots. The results indicated that the n-hexane, chloroform, and ethyl acetate extracts of P. ostreatus contained bioactive compounds belonging to the alkaloid, flavonoid, and terpenoid groups. The n-hexane extract exhibited cytotoxic effects against T47D cells with an IC50 value of 990.78 µg/mL and against MCF7 cells with an IC50 value of 555.22 µg/mL. The chloroform extract demonstrated cytotoxic effects against T47D cells with an IC50 value of 21.564 µg/mL and against MCF7 cells with an IC50 value of 371.15 µg/mL. The ethyl acetate extract showed cytotoxic effects against T47D cells with an IC50 value of 3.226 µg/mL and against MCF7 cells with an IC50 value of 371.15 µg/mL.
