THE EFFECT OF VARIATION OF IMMOBILIZATION TIME AND CONCENTRATION ROCELLA (Hibiscus sabdariffa L.) EXTRACT ON CHARACTERISTICS AND EVALUATION OF TIME TEMPERATURE INDICATOR

Abstract

Anthocyanin is a type of colour pigment that has the potential as an indicator for making a Time Temperature Indicator (TTI). This is because anthocyanins are stable at low temperatures and change colour due to temperature changes (thermochromic). In this study, an analysis was carried out to determine the combination of various concentrations of roselle extract and immobilization time and to evaluate the colour stability of TTI. The research was carried out for 5 months at the Unsoed Agricultural Technology Laboratory. The combined treatment used RAK with roselle extract concentration factors of 17,5%, 20% and 22,5% and immobilization time factors of 1, 2 and 3 hours. Then the colour stability of a* TTI was observed. Variable measurements were carried out 3 times. Making TTI was using analysis of variance with a value of α 5% followed by DMRT test with α 5% if it was significantly different. While the evaluation of colour stability is analysis using linear regression. The selected TTI in this study was a combination of 22,5% concentration and 2 hours of immobilization. This treatment had a total absorption value of 1,56 g, total anthocyanin of 25,77 μg/mL, L* colour of 72.19, a* colour of 8,53, b* colour of 6.44 and ΔE of 10,98. Evaluation of TTI anthocyanin stability at 50o C had the highest decrease in a* value of 2,798 and the lowest retention value at 12 hours of observation time of 91.58%.