FORMULATION OF LINDUR LEAVES (Bruguiera gymnorhiza) IN NANOPARTICLE FORM USING CARBOXYMETYL-CHITOSAN

Abstract

Abstract. Analgesic, antioxidant, anti-inflammatory, anti-cancer, anti-bacterial, and anti-fungal advantages have been reported with a mangrove plant known as lindur (Bruguria gymnorhiza), which contains alkaloids, tannins, saponins, and steroids. The molecular size and solubility of this compound is very large. By the use of nanoparticle technology, it is possible to make medications more soluble by increasing the surface area of the particles. Carboxymethyl-chitosan (CMChi) polymer and CaCl2 are both used in the manufacturing of these nanoparticle. CMChi polymer concentrations of 200, 300, and 400 mg are used in the preparation of Lindur leaf extract nanoparticles, together with CaCl2 as a crosslinker. The ionic gelation-freeze drying technique was used to create nanoparticles. The obtained nanoparticles were evaluated for size and particle size distribution (PI), zeta potential, morphology and FTIR analysis. The smallest size and most uniform particle size distribution were formed by nanoparticles containing 200 mg of CMChi. The generated square nanoparticles are 196,5 ± 29,56 nm in size. Their PI values are 0,252 ± 0,053 and their zeta potentials are -17,43 ± 1.01 mV. According to the findings of the FT-IR study, the functional groups in the nanoparticles and the extract from B. gymnorhiza leaf are comparable.