Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune
Abstract
Schizophyllum commune is a fungus that grows naturally on tree trunks and wood processing waste. This mushroom contains β-glucan compounds which have the potential for drug development in the world of health. The main purpose of this study was to determine the optimum value of pH and incubation time on S. commune's growth, and also the optimum value of pH and incubation time on β-glucan production of S. commune. The research was conducted by experimental method of completely randomized factorial design (CRD factorial). The treatment given includes variations in pH (P) with three levels, namely pH 5 (P1), pH 6 (P2), and pH 7 (P3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (W1), 25 days (W2), and 30 days (W3). . The independent variables were pH and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that pH and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. The value of pH 6 and incubation time of 25 days was the optimum condition for the growth of S. commune, and also pH 5 and incubation time of 25 days was the optimum condition for β-glucan production of S. commune.
References
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Candida sp Dan Aspergillus sp Dari Bilasan
Bronkus Penderita Tuberkulosis Paru pada
Media Bekatul. Jurnal Ilmu Alam Dan
Lingkungan, 9(18), pp.74-82.
Bellettini, M.B., Fiorda, F.A., Maieves, H.A.,
Teixeira, G.L., Ávila, S., Hornung, P.S.,
Júnior, A. M. & Ribani, R. H., 2019. Factors
Affecting Mushroom Pleurotus Spp. Saudi
Journal of Biological Sciences, 26(4), pp.633-
646.
Chihara, G., Hamuro, J. & Maeda, Y., 1987.
Antitumor and Metastatis-Inhibitory
Activities of Lentinan as an
Immunomodulator: An Overview. Cancer
Detect Prev.19(1), pp.423-443.
Devi, M.B., Mukta, S.S. & Irabanta, N.S., 2015.
Biomass Production in Auricularia spp. (Jews
ear) Collected from Manipur, India. Int J Curr
Microbiol App Sc., 4(6), pp.985-989.
Ekowati, N., Ina, R,N. & Mumpuni, A., 2016. Potensi
Jamur Trametes versicolor dan Russula sp.
dalam Menghasilhan β-Glukan Melalui Proses
Fermentasi. Seminar Nasional Pendidikan dan
Saintek. Universitas Muhamadiyah Surakarta.
Elisashvili, V.I., 2012. Submerged Cultivation of
Medicinal Mushrooms: Bioprocesses and
Products. International journal of medicinal
mushrooms, 14(3), pp.211-239.
Hasan, F.R.H. & Ghada, M.M., 2012. Studies on
Submerged Culture Conditions for Mycelial
Biomass Production of Wood Ears Mushroom
(Auricularia polytricha). Middle East Journal
of Agriculture Research, 1(1), pp.33-39.
Herliyana, E.N., Maryam, L.F. & Hadi, Y.S., 2011.
Schizophyllum commune Fr. Sebagai Jamur
Uji Ketahanan Kayu Standar Nasional
Indonesia pada Empat Jenis Kayu Rakyat:
Sengon (P. falcataria), Karet (H. brasiliensis),
Tusam (P. merkusii), Mangium (A. mangium). Jurnal Silvikultur Tropika, 2(3),
pp.176-180.
Johnsy, G. & Kaviyarasan, V., 2013. Effect of
Physico-Chemical Factors and SemiSynthetic Media on Vegetative Growth of
Neolentinus Kauffmanii an Edible Mushroom
from Kanyakumari District. Int J Pharm Bio
Sci, 4(1), pp.469-478.
Joruszuk, M.O., Wilkolazka, A.J., Scisel, J.J., 2015.
Extracellular Polysaccharides from
Ascomycota and Basidiomycota: Production
Conditions, Biochemical Characteristics, and
Biological Properties. World J Microbiol
Biotechnol. 31, pp.1823-1844.
Joshi, M., Patel, H., Gupte, S. & Gupte, A., 2013.
Nutrient Improvement for Simultaneous
Production of Exopolysaccharide and
Mycelial Biomass by Submerged Cultivation
of Schizophyllum commune Agmj-1 Using
Statistical Optimization. 3 Biotech, 3(4),
pp.307-318.
Kadnikova, I.A., Costa, R., Kalenik, T.K., Guruleva,
O. N. & Yanguo, S., 2015. Chemical
Composition and Nutritional Value of the
Mushroom Auricularia Auriculajudae.
Journal of Food and Nutrition Research, 3(8),
pp.478–482.
Kim, S.W., Hwang, H.J., Park, J.P., Cho, Y.J., Song,
C. H. & Yun, J. W., 2002. Mycelial Growth
and Exo‐Biopolymer Production By
Submerged Culture of Various Edible
Mushrooms Under Different Media. Letters In
Applied Microbiology, 34(1), pp.56-61.
Krisnawati, Y. & Fitriani, L., 2020. Pengembangan
Lembar Kerja Mahasiswa (LKM) Berbasis
Eksplorasi Jamur Makroskopis. Bioedusains:
Jurnal Pendidikan Biologi dan Sains, 3(1),
pp.8-23.
Lai, W.H., Salleh, S.M., Daud, F., Zainal, Z.,
Othman, A.M. & Saleh, N.M., 2014.
Optimization of Submerged Culture
Conditions for the Production of Mycelial
Biomass and Exopolysaccharides from
Lignosus rhinocerus. Sains
Malaysiana, 43(1), pp.73-80.
Mohamad, S.A., Awang, R.M., Ibrahim, R., Keong,
R., Hamzah., Rashid, R.A., Hussein, S.,
Rahim, A., Daud, A., Hamid, A. & Yusof, W.,
2015. Production of Endopolysaccharides
from Malaysia’s Local Mushrooms in Air-Lift
Bioreactor. Bioscience and Biotechnology, 6,
pp.456-46.
Muthu, N & Shanmugasundaram, K., 2015. Effect of
Five Different Culture Media on Mycelial
Growth of Agrocybe Aegerita. International
Journal of Pharmaceutical Sciences and
Research (IJPSR), 6(12), pp.5193-5197.
Nasreen, Z., Khan, S.J., Ammara, Y., Muafia, S.,
Shumaila, U. & Sakhawat, A., 2015.
Optimization of Sub-Merged Culture
Conditions for Biomass Production
Schizophyllum commune, a Medicinal
Mushroom. International Journal of Current
Microbiology and Applied Sciences, 4(2),
pp.258-266.
Pelczar, M.J. & Chan, E.C.S., 1986. Dasar-Dasar
Mikrobiologi. Jakarta: Universitas Indonesia
Press
Purwati, E., 2017. Optimasi Medium Pertumbuhan
Dan Waktu Inkubasi Terhadap Bobot
Miselium Schizophyllum commune dan
Golongan Metabolit Sekunder yang
Dihasilkan. Skripsi. Purwokerto: Fakultas
Biologi Universitas Jenderal Soedirman.
Rahayu, AM., 2018. Pengaruh Jenis Medium Dan
Waktu Inkubasi Terhadap Pertumbuhan Jamur
Auricularia auricula Serta Deteksi Golongan
Metabolit Sekunder yang Dihasilkan. Skripsi.
Purwokerto: Fakultas Biologi Universitas
Jenderal Soedirman.
Rahma, K., Mahdi, N. & Hidayat, M., 2019.
Karakteristik Jamur Makroskopis Di
Perkebunan Kelapa Sawit Kecamatan
Meureubo Aceh Barat. Prosiding Biotik, 5(1),
pp.157-164.
Rahmi, D., Ratnawati, E., Yunilawati, R. & Aidha, N.
N., 2014. Peningkatan Aktivitas Anti Aging
pada Krim Nanopartikel dengan Penambahan
Bahan Aktif Alam. Jurnal Kimia dan
Kemasan, 36(2), pp.215-224.
Rosnan, N.D.R., Chuen, N. & Ngadin, A.A., 2019.
First Record of in Vitro Growth Evaluation of
Wild Mushroom, Schizophyllum commune
from Pulau Kapas in Malaysia. Asian Journal
Of Agriculture And Biology, 7(4), pp.602-609.
Saskiawan, I., Munir, M. & Achmadi, S.S., 2017.
Optimasi Produksi Serta Analisis Aktivitas
Antioksidan dan Antimikroba Senyawa
Eksopolisakarida dari Jamur Tiram Putih
(Pleurotus ostreatus) pada Media Cair. Berita
Biologi Jurnal Ilmu-Ilmu Hayati, 15(2),
pp.133-140.
Shah, P. & Modi, H.A., 2018. Optimization of
Culture Conditions for Biomass Production of
Ganoderma lucidum. International Journal of
Current Microbiology and Applied
Sciences, 7(02), pp.1882-1889.
Syarifah, N.D.T., Ekowati, N., Mumpuni, A. &
Saskiawan, I., 2021. Detection of Secondary Metabolite of Mycena pelianthina Growth In
Various Liquid Medium. Journal of
Functional Food and Nutraceutical, 2(2),
pp.89-97.
Tampubolon, M.B., Utomo, B. & Yunasfi., 2012.
Keanekaragaman Jamur Makroskopis Di
Hutan Pendidikan Universitas Sumatera Utara
Desa Tongkoh Kabupaten Karo Sumatera
Utara. Saintia Biologi, 2, pp.176-182.
Tampubolon., Santa, D.B.M., Utomo, B. & Yunasfi.,
2015. Keanekaragaman Jamur Makroskopis
Di Hutan Pendidikan Universitas Sumatera
Utara Desa Tongkoh Kabupaten Karo
Sumatera Utara. Artikel Ilmiah. Medan:
Universitas Sumatera Utara.
Widiastuti, H., Siswanto. & Suharyanto., 2007.
Optimasi Pertumbuhan Dan Aktivitas Enzim
Ligninolitik Omphalina Sp. Dan Pleurotus
ostreatus pada Fermentasi Padat. Menara
Perkebunan, 75(2), pp.93-105.
Widyastuti, N., 2013. Pengolahan Jamur Tiram
(Pleurotus ostreatus) Sebagai Alternatif
Pemenuhan Nutrisi. Jurnal Sains dan
Teknologi Indonesia, 15(3), pp.1-7.
Widyastuti, N., Baruji, T., Giarni, R., Isnawan, H.,
Wahyudi, P. & Donowati., 2011. Analisa
Kandungan Beta-Glukan Larut Air Dan Larut
Alkali dari Tubuh Buah Jamur Tiram
(Pleurotus ostreatus) Dan Shiitake (Lentinus
edodes). Jurnal Sains dan Teknologi
Indonesia, 13(3), pp.182-191.
Yap, A.T. & Ng, M.L.M., 2001. An Improved
Methode for the Isolation of Lentinan from
Lentinula edodes (Berk) Sing
(Agaricomycetidae). International Journal of
Medical Mushroom, 3, pp.6-19.
Yenie, E. & Utami, S.P., 2018. Pengaruh Suhu Dan
pH Pertumbuhan Jamur Merang (Volvariella
volvacea) Terhadap Degradasi Lignin Tandan
Kosong Kelapa Sawit. Jurnal APTEK, 10(1),
pp.29-35.
Candida sp Dan Aspergillus sp Dari Bilasan
Bronkus Penderita Tuberkulosis Paru pada
Media Bekatul. Jurnal Ilmu Alam Dan
Lingkungan, 9(18), pp.74-82.
Bellettini, M.B., Fiorda, F.A., Maieves, H.A.,
Teixeira, G.L., Ávila, S., Hornung, P.S.,
Júnior, A. M. & Ribani, R. H., 2019. Factors
Affecting Mushroom Pleurotus Spp. Saudi
Journal of Biological Sciences, 26(4), pp.633-
646.
Chihara, G., Hamuro, J. & Maeda, Y., 1987.
Antitumor and Metastatis-Inhibitory
Activities of Lentinan as an
Immunomodulator: An Overview. Cancer
Detect Prev.19(1), pp.423-443.
Devi, M.B., Mukta, S.S. & Irabanta, N.S., 2015.
Biomass Production in Auricularia spp. (Jews
ear) Collected from Manipur, India. Int J Curr
Microbiol App Sc., 4(6), pp.985-989.
Ekowati, N., Ina, R,N. & Mumpuni, A., 2016. Potensi
Jamur Trametes versicolor dan Russula sp.
dalam Menghasilhan β-Glukan Melalui Proses
Fermentasi. Seminar Nasional Pendidikan dan
Saintek. Universitas Muhamadiyah Surakarta.
Elisashvili, V.I., 2012. Submerged Cultivation of
Medicinal Mushrooms: Bioprocesses and
Products. International journal of medicinal
mushrooms, 14(3), pp.211-239.
Hasan, F.R.H. & Ghada, M.M., 2012. Studies on
Submerged Culture Conditions for Mycelial
Biomass Production of Wood Ears Mushroom
(Auricularia polytricha). Middle East Journal
of Agriculture Research, 1(1), pp.33-39.
Herliyana, E.N., Maryam, L.F. & Hadi, Y.S., 2011.
Schizophyllum commune Fr. Sebagai Jamur
Uji Ketahanan Kayu Standar Nasional
Indonesia pada Empat Jenis Kayu Rakyat:
Sengon (P. falcataria), Karet (H. brasiliensis),
Tusam (P. merkusii), Mangium (A. mangium). Jurnal Silvikultur Tropika, 2(3),
pp.176-180.
Johnsy, G. & Kaviyarasan, V., 2013. Effect of
Physico-Chemical Factors and SemiSynthetic Media on Vegetative Growth of
Neolentinus Kauffmanii an Edible Mushroom
from Kanyakumari District. Int J Pharm Bio
Sci, 4(1), pp.469-478.
Joruszuk, M.O., Wilkolazka, A.J., Scisel, J.J., 2015.
Extracellular Polysaccharides from
Ascomycota and Basidiomycota: Production
Conditions, Biochemical Characteristics, and
Biological Properties. World J Microbiol
Biotechnol. 31, pp.1823-1844.
Joshi, M., Patel, H., Gupte, S. & Gupte, A., 2013.
Nutrient Improvement for Simultaneous
Production of Exopolysaccharide and
Mycelial Biomass by Submerged Cultivation
of Schizophyllum commune Agmj-1 Using
Statistical Optimization. 3 Biotech, 3(4),
pp.307-318.
Kadnikova, I.A., Costa, R., Kalenik, T.K., Guruleva,
O. N. & Yanguo, S., 2015. Chemical
Composition and Nutritional Value of the
Mushroom Auricularia Auriculajudae.
Journal of Food and Nutrition Research, 3(8),
pp.478–482.
Kim, S.W., Hwang, H.J., Park, J.P., Cho, Y.J., Song,
C. H. & Yun, J. W., 2002. Mycelial Growth
and Exo‐Biopolymer Production By
Submerged Culture of Various Edible
Mushrooms Under Different Media. Letters In
Applied Microbiology, 34(1), pp.56-61.
Krisnawati, Y. & Fitriani, L., 2020. Pengembangan
Lembar Kerja Mahasiswa (LKM) Berbasis
Eksplorasi Jamur Makroskopis. Bioedusains:
Jurnal Pendidikan Biologi dan Sains, 3(1),
pp.8-23.
Lai, W.H., Salleh, S.M., Daud, F., Zainal, Z.,
Othman, A.M. & Saleh, N.M., 2014.
Optimization of Submerged Culture
Conditions for the Production of Mycelial
Biomass and Exopolysaccharides from
Lignosus rhinocerus. Sains
Malaysiana, 43(1), pp.73-80.
Mohamad, S.A., Awang, R.M., Ibrahim, R., Keong,
R., Hamzah., Rashid, R.A., Hussein, S.,
Rahim, A., Daud, A., Hamid, A. & Yusof, W.,
2015. Production of Endopolysaccharides
from Malaysia’s Local Mushrooms in Air-Lift
Bioreactor. Bioscience and Biotechnology, 6,
pp.456-46.
Muthu, N & Shanmugasundaram, K., 2015. Effect of
Five Different Culture Media on Mycelial
Growth of Agrocybe Aegerita. International
Journal of Pharmaceutical Sciences and
Research (IJPSR), 6(12), pp.5193-5197.
Nasreen, Z., Khan, S.J., Ammara, Y., Muafia, S.,
Shumaila, U. & Sakhawat, A., 2015.
Optimization of Sub-Merged Culture
Conditions for Biomass Production
Schizophyllum commune, a Medicinal
Mushroom. International Journal of Current
Microbiology and Applied Sciences, 4(2),
pp.258-266.
Pelczar, M.J. & Chan, E.C.S., 1986. Dasar-Dasar
Mikrobiologi. Jakarta: Universitas Indonesia
Press
Purwati, E., 2017. Optimasi Medium Pertumbuhan
Dan Waktu Inkubasi Terhadap Bobot
Miselium Schizophyllum commune dan
Golongan Metabolit Sekunder yang
Dihasilkan. Skripsi. Purwokerto: Fakultas
Biologi Universitas Jenderal Soedirman.
Rahayu, AM., 2018. Pengaruh Jenis Medium Dan
Waktu Inkubasi Terhadap Pertumbuhan Jamur
Auricularia auricula Serta Deteksi Golongan
Metabolit Sekunder yang Dihasilkan. Skripsi.
Purwokerto: Fakultas Biologi Universitas
Jenderal Soedirman.
Rahma, K., Mahdi, N. & Hidayat, M., 2019.
Karakteristik Jamur Makroskopis Di
Perkebunan Kelapa Sawit Kecamatan
Meureubo Aceh Barat. Prosiding Biotik, 5(1),
pp.157-164.
Rahmi, D., Ratnawati, E., Yunilawati, R. & Aidha, N.
N., 2014. Peningkatan Aktivitas Anti Aging
pada Krim Nanopartikel dengan Penambahan
Bahan Aktif Alam. Jurnal Kimia dan
Kemasan, 36(2), pp.215-224.
Rosnan, N.D.R., Chuen, N. & Ngadin, A.A., 2019.
First Record of in Vitro Growth Evaluation of
Wild Mushroom, Schizophyllum commune
from Pulau Kapas in Malaysia. Asian Journal
Of Agriculture And Biology, 7(4), pp.602-609.
Saskiawan, I., Munir, M. & Achmadi, S.S., 2017.
Optimasi Produksi Serta Analisis Aktivitas
Antioksidan dan Antimikroba Senyawa
Eksopolisakarida dari Jamur Tiram Putih
(Pleurotus ostreatus) pada Media Cair. Berita
Biologi Jurnal Ilmu-Ilmu Hayati, 15(2),
pp.133-140.
Shah, P. & Modi, H.A., 2018. Optimization of
Culture Conditions for Biomass Production of
Ganoderma lucidum. International Journal of
Current Microbiology and Applied
Sciences, 7(02), pp.1882-1889.
Syarifah, N.D.T., Ekowati, N., Mumpuni, A. &
Saskiawan, I., 2021. Detection of Secondary Metabolite of Mycena pelianthina Growth In
Various Liquid Medium. Journal of
Functional Food and Nutraceutical, 2(2),
pp.89-97.
Tampubolon, M.B., Utomo, B. & Yunasfi., 2012.
Keanekaragaman Jamur Makroskopis Di
Hutan Pendidikan Universitas Sumatera Utara
Desa Tongkoh Kabupaten Karo Sumatera
Utara. Saintia Biologi, 2, pp.176-182.
Tampubolon., Santa, D.B.M., Utomo, B. & Yunasfi.,
2015. Keanekaragaman Jamur Makroskopis
Di Hutan Pendidikan Universitas Sumatera
Utara Desa Tongkoh Kabupaten Karo
Sumatera Utara. Artikel Ilmiah. Medan:
Universitas Sumatera Utara.
Widiastuti, H., Siswanto. & Suharyanto., 2007.
Optimasi Pertumbuhan Dan Aktivitas Enzim
Ligninolitik Omphalina Sp. Dan Pleurotus
ostreatus pada Fermentasi Padat. Menara
Perkebunan, 75(2), pp.93-105.
Widyastuti, N., 2013. Pengolahan Jamur Tiram
(Pleurotus ostreatus) Sebagai Alternatif
Pemenuhan Nutrisi. Jurnal Sains dan
Teknologi Indonesia, 15(3), pp.1-7.
Widyastuti, N., Baruji, T., Giarni, R., Isnawan, H.,
Wahyudi, P. & Donowati., 2011. Analisa
Kandungan Beta-Glukan Larut Air Dan Larut
Alkali dari Tubuh Buah Jamur Tiram
(Pleurotus ostreatus) Dan Shiitake (Lentinus
edodes). Jurnal Sains dan Teknologi
Indonesia, 13(3), pp.182-191.
Yap, A.T. & Ng, M.L.M., 2001. An Improved
Methode for the Isolation of Lentinan from
Lentinula edodes (Berk) Sing
(Agaricomycetidae). International Journal of
Medical Mushroom, 3, pp.6-19.
Yenie, E. & Utami, S.P., 2018. Pengaruh Suhu Dan
pH Pertumbuhan Jamur Merang (Volvariella
volvacea) Terhadap Degradasi Lignin Tandan
Kosong Kelapa Sawit. Jurnal APTEK, 10(1),
pp.29-35.
Published
2022-06-27
How to Cite
MAHARANI, Amalia Sofia; EKOWATI, Nuraeni; RATNANINGTYAS, Nuniek Ina.
Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune.
BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 4, n. 1, p. 38-45, june 2022.
ISSN 2714-8564.
Available at: <https://jos.unsoed.ac.id/index.php/bioe/article/view/4594>. Date accessed: 13 mar. 2025.
doi: https://doi.org/10.20884/1.bioe.2022.4.1.4594.
Section
Articles
