Aktivitas Amilolitik Bakteri Sedimen Mangrove Pantai Logending, Ayah, Kebumen pada Suhu dan pH Berbeda
Abstract
Mangrove sediment is a source of various microorganisms that degrade leaf litter or parts of mangrove plants, including amylolytic bacteria. Bacteria isolated from the mangrove sediments of the Logending beach have not yet known their amylolytic ability. Optimum amylase activity can be influenced by temperature and pH. This study aims to determine the ability of mangrove sediment bacterial isolates to produce amylase enzymes, to determine the pH and temperature for optimum amylase activity, and to identify amylase-producing bacteria. The research was conducted by survey method. The research stages included screening for amylolytic bacteria, preparation of growth curves, amylase production, optimizing amylolytic activity at various temperatures (35ºC; 36ºC; 37ºC; 38ºC) and pHs (4.5; 5; 5.5; 6) and characterization of amylolytic isolates. The parameters measured were amylolytic index, amylase activity quantitatively, total number of bacteria, and identity of amylolytic bacteria from mangrove sediments. Data analysis was done descriptively. The results showed that the highest amylolytic index were shown by isolate LG113 with an amylolytic index value of 9.86 from 10 amylolytic isolates from mangrove sediments. The optimum temperature for amylase activity was 37ºC, which was 2.13 U/mL and the optimum pH was 6, which was 2.14 U/mL. The total number of amylolytic bacterial cells at the end of the production period was 1.94.1013 cfu/mL. The amylolytic isolate LG113 belongs to Bacillus genera.
References
Behera, B. C., Singdevsachan, S. K., Mishra, R. R., Sethi, B. K., Dutta, S. K. & Thatoi, H. N., 2016. Phosphate Solubilising Bacteria from Mangrove Soils of Mahanadi River Delta, Odisha, India. World journal of agricultural research, 4(1), pp. 18-23.
Cappuccino, J. G. & Sherman, N. 2014. Microbiology: A Laboratory Manual, 10th Edition. England: Pearson Education Press.
Carvalho, R. V. D., Correa, T. L. R., Silva, J. C. M. D., Mansur, L. R. C. D. O. & Martins, M. L. L., 2008. Properties of an Amylase from Thermophilic Bacillus sp. Brazilian Journal of Microbiology, 39(1), pp. 102-107.
Darmayasa I. B. G., 2008. Isolasi dan Identifikasi Bakteri Pendegradasi Lipid (Lemak) pada Beberapa Tempat Pembuangan Limbah dan Estuari DAM Denpasar. Jurnal Bumi Lestari, 8(2), pp. 122-127.
Fatoni, A. & Zusfahair. 2012. Thermophilic Amylase from Thermus sp. Isolation and Its Potential Application for Bioethanol Production. Songklanakarin J. Sci. Technol. 34 (5), pp. 525-531
Flori, F., Mukarlina, M. & Rahmawati, R., 2020. Karakterisasi Bacillus spp. dan Fusarium sp. dari Tanaman Lada (Piper nigrum L.) di Desa Jaga. Protobiont, 9(1), pp. 50-55.
Habibie, F. M., Wardani, A. K.& Nurcholis, M., 2014. Isolasi dan Identifikasi Molekuler Mikroorganisme Termofilik Penghasil Xilanase dari Lumpur Panas Lapindo. Jurnal Pangan dan Agroindustri, 2(4), pp. 231-238.
Islamiah, D. N. & Rahmawati, R. L., 2017. Jenis-jenis Bakteri Rizosfer Kawasan Tanah Mangrove Avicennia di Kelurahan Terusan, Kecamatan Mempawah Hilir, Kalimantan Barat. Protobiont, 6(3), pp. 165-172.
Istia'nah, D., Utami, U. & Barizi, A., 2020. Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat. Jurnal Riset Biologi dan Aplikasinya, 2(1), pp. 11-17.
Kresnawaty, I., Wahyu, R. & Sasongko, A., 2019. Aktivitas Amilase Bakteri Amilolitik Asal Larva Black Soldier Fly (Hermetia illucens). Menara Perkebunan, 87(2), pp. 140-146.
Nurfajriyah, S., Inggraini, M. & Ilsan, N. A., 2017. Skrinning Rhizobakteri Mangrove Rhizosphora sp. Penghasil Amilase. Jurnal Mitra Kesehatan, 1(1), pp. 12-17.
Pramono, H., Mariana, A., Ryandini, D., Sudiana, E. 2021. Diversity of Cellulolytic Bacteria Isolated from Coastal Mangrove Sediment in Logending Beach, Kebumen, Indonesia. Biodiversitas 22(4), pp.1869-1878
Purnawan, A., Capriyanti, Y., Kurniatin, P. A. & Rahmani, N., 2016. Optimasi Produksi Enzim Amilase dari Bakteri Laut Jakarta (Arthrobacter arilaitensis). Jurnal Biologi Indonesia, 11(2), pp. 215-244.
Puspita, F., Ali, M. & Pratama, R., 2017. Isolasi dan Karakterisasi Morfologi dan Fisiologi Bakteri Bacillus sp. Endofitik dari Tanaman Kelapa Sawit (Elaeis guineensis Jacq.). Jurnal Agroteknologi Tropika, 6(2), pp. 44-49.
Rahayuningsih, T., Isnatin, U. & Parwi, P., 2018. Isolasi dan Seleksi Bakteri sebagai Agen Bioremediasi Limbah Cair Pabrik Kayu Putih. Jurnal Agri-Tek: Jurnal Penelitian Ilmu-Ilmu Eksakta, 19(2), pp. 96-99.
Rismawati, Y., Bahri, S. & Prismawiryanti, P., 2016. Produksi Glukosa dari Jerami Padi (Oryza sativa) Menggunakan Jamur Trichoderma sp. KOVALEN: Jurnal Riset Kimia, 2(2), pp. 67-76.
Rodriguez, V. B., Alameda, E. J., Gallegos, J. M., Requena, A. R. & Lopez, A. G., 2006. Enzymatic Hydrolysis of Soluble Starch with an α‐Amylase from Bacillus licheniformis. Biotechnology progress, 22(3), pp. 718-722.
Sahoo, K. & Dhal, N.K. &. 2009. Potential Microbial Diversity in Mangrove Ecosystems: A Review. Indian Journal of Geo-Marine Sciences 38(2), pp. 249-256.
Silitonga, L. R., Nursyirwani, N. & Effendi, I., 2019. Isolation, Identification and Sensitivity of Amylolytic Bacteria from Mangrove Ecosystem Sediment in Purnama Marine Station Dumai on the Pathogenic Bacteria. Asian Journal of Aquatic Sciences, 2(3), pp. 257-266.
de Souza, P.M. & de Oliveira e Magalhães, P. 2010. Application of Microbial α-Amylase in Industry—A Review. Brazilian Journal of Microbiology, 41, pp. 850-861
Subagiyo, S., Djarod, M. S. R. & Setyati, W. A., 2017. Potensi Ekosistem Mangrove sebagai Sumber Bakteri untuk Produksi Protease, Amilase dan Selulase. Jurnal Kelautan Tropis, 20(2), pp. 106-111.
Sudin, S., Sulistijowati, R. & Hermain, R. M. 2020. Penapisan dan Pola Pertumbuhan Bakteri Kitinolitik dari Cangkang Rajungan (Portunus pelagicus). Jambura Fish Processing Journal, 2(1), pp. 36-45.
Supriyanti, F. M. T. & Heryanto, T. E., 2013. Penentuan Aktivitas Amilase Kasar Termofil Bacillus subtilis Isolat Kawah Gunung Darajat Garut, Jawa Barat. Bionatura Jurnal Ilmu-ilmu Hayati dan Fisik, 15(2), pp. 107-113.
Triandini, I. G. A. A. H. & Suryadi, B. F., 2018. Uji Aktivitas dan Produksi Antibakteri Bacillus lentus yang diisolasi dari Sistem Pencernaan Landak Laut dalam Menghambat Bakteri Penyebab Infeksi pada Kehamilan. Jurnal Sangkareang Mataram, 4(2), pp. 34-40.
Vaikundamoorthy, R., Rajendran, R., Selvaraju, A., Moorthy, K. & Perumal, S., 2018. Development of Thermostable Amylase Enzyme from Bacillus cereus for Potential Antibiofilm Activity. Bioorganic chemistry, 77, pp. 494-506.
Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A. & Whitman, W. B., 2009. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes.2nd Edition.New York: Springer Science & Business Media.
Wahyuningsih, S. S., Mursyanti, E. & Atmodjo, P. K., 2019. Pola Pertumbuhan dan Produksi α-Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 9(2), pp. 84-91.
Wulandhari, P. S., Rachmawati, D. & Susilowati, T. 2017. Pengaruh Kombinasi Ekstrak Nanas dalam Pakan Buatan dan Probiotik pada Media terhadap Efisiensi Pemanfaatan Pakan dan Pertumbuhan Ikan Tawes (Puntius javanicus). Journal of Aquaculture Management and Technology, 6(4), pp. 157-166.
Yasin, M. N., Astuti, W. & Gunawan, R., 2017. Screening Bakteri Penghasil Amilase dari Sedimen Sumber Air Panas Dondang Muara Jawa. Jurnal Atomik, 2(2), pp. 213-215.
Zubaidah, A., Prasetyo, D., Handajani, H., Rohmah, S. P. & Puspita, D. A., 2019. Screening Bakteri Selulolitik dan Amilolitik pada Rumen Sapi sebagai Kandidat Probiotik Pada Budidaya Ikan secara In Vitro. Jurnal Riset Akuakultur, 14(4), pp. 261-271