Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami

  • Nuridha Audinia Safitri Fakultas Biologi, Universitas Jenderal Soedirman
  • Atang Atang Fakultas Biologi, Universitas Jenderal Soedirman
  • Eko Setio Wibowo

Abstract

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.

References

Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C. & Chen, K., 2007. Bioactive Compounds and Antioxidant Capacities in Different Edible Tissues of Citrus Fruit of Four Species. Food Chemistry, 104(1), pp.1338–1344.
Amalia, N., 2009. Uji Toksisitas Akut Ekstrak Valerian (Valeriana officinalis) terhadap Hepar Mencit BALB/C. Semarang: Fakultas Kedokteran, Universitas Diponegoro.
Anggraeny, E., Tjandrakirana & Ducha, N., 2014. Pengaruh Pemberian Filtrat Tauge Kacang Hijau terhadap Histologi Hepar Mencit yang Terpapar MSG. LenteraBio, 3(3), pp.186–191.
Cahyani, D.I., Martino, Y.A. & Purnomo, Y., 2021. Efek Dekokta Daun Pulutan (Urena lobata) terhadap Kadar Superoxide Dismutase (SOD) dan Malondialdehyde (MDA) Hepar Ikan Zebra (Danio rerio) Fase Juvenile yang Dipapar Malathion Kronik. Jurnal Kedokteran Komunitas, 9(1), pp.1–6.
Fitriani, R.N., Sitasiwi, A.J. & Isdadiyanto, S., 2020. Struktur Hepar dan Rasio Bobot Hepar terhadap Bobot Tubuh Mencit (Mus Musculus L.) Jantan setelah Pemberian Esktrak Etanol Daun Mimba (Azadirachta Indica A.Juss). Buletin Anatomi dan Fisiologi, 5(1), pp.75–83.
Fretes, H.D., Susanto, A.B., Prasetyo, B. & Limantara, L., 2012. Karotenoid dari Makroalgae dan Mikroalgae: Potensi Kesehatan Aplikasi dan Bioteknologi. Jurnal Teknologi dan Industri Pangan, 23(2), pp.221–228.
Husna, N.E., Novita, M. & Rohaya, S., 2013. Kandungan Antosianin dan Aktivitas Antioksidan Ubi Jalar Ungu Segar dan Produk Olahannya. Agritech, 33(3), pp.296–302.
Ilmiawati, C., Reza, M., Rahmatini & Rustam, E., 2017. Edukasi Pemakaian Plastik sebagai Kemasan Makanan dan Minuman serta Risikonya terhadap Kesehatan pada Komunitas di Kecamatan Bungus Teluk Kabung, Padang. LOGISTA-Jurnal Ilmiah Pengabdian kepada Masyarakat, 1(1), pp.20–28.
Jambeck, J.R., Geyer, R., Wilcox, C., Siegler, T.R., Perryman, M., Andrady, A., Narayan, R. & Law, K.L., 2015. Plastic Waste Inputs from Land into The Ocean. Science, 347(6223), pp.768–771.
Juanda, D., Budiana, W. & Ridwan, I.M., 2015. Penetapan Kadar Total Fenol dan Aktivitas Antioksidan dari Jus Buah Lima Spesies Jeruk (Citrus sp.). Jurnal Farmasi Galenika, 2(1), pp.36–42.
Karuniastuti, N., 2013. Bahaya Plastik terhadap Kesehatan dan Lingkungan. Forum Teknologi, 3(1), pp.6–14.
Makiyah, A. & Khumaisah, L.L., 2018. Studi Gambaran Histopatologi Hepar Tikus Putih Strain Wistar yang Diinduksi Aspirin Pascapemberian Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl.) Selama 7 Hari. Majalah Kedokteran Bandung, 50(2), pp.93–101.
Mangunsong, S., Assiddiqy, R., Sari, E.P., Marpaung, P.N. & Sari, R.A., 2019. Penentuan β-karoten dalam Buah Wortel (Daucus carota) secara Kromatografi Cair Kinerja Tinggi (U-HPLC). AcTion: Aceh Nutrition Journal, 4(1), pp.36–41.
Mau, F. & Desato, Y., 2013. Studi Kualitas (Quality Assurance) Pemeriksaan Mikroskopis Malaria Di Pulau Sumba Tahun 2009. Jurnal Ekologi Kesehatan, 12(2), pp. 79–86.
Maulida, A., Ilyas, S. & Hutahaean, S., 2013. Pengaruh Pemberian Vitamin C dan E terhadap Gambaran Histologis Hepar Mencit (Mus Musculus L.) yang Dipajankan Monosodium Glutamat (MSG). Saintia Biologi, 1(2), pp.15–20.
Paramita, N.P.C., Sugiritama, I.W., Linawati, N.M., Ratnayanti, IGA.D., Wahyuniari, I.A.I., Arijana, IGK.N. & Wiryawan, IGN.S., 2019. Ekstrak Etanol Ubi Jalar Ungu (Ipomoea Batatas L.) Menurunkan Degenerasi Lemak Jaringan Hati Tikus yang Diovariektomi. E-Jurnal Medika, 8(5), pp.1–10.
Parwata, I.M.O.A., 2016. Antioksidan. Bali: Universitas Udayana.
Perdana, W.Y. & Jacobus, D.J., 2016. Bisphenol A (BPA) adalah Endocrine Disrupture Chemicals (EDC) yang Berperan sebagai Agen Diabetogenik. CDK-244, 43(9), pp.706–711.
Pramesti, N.K.T., Wiratmini, N.I. & Astiti, N.P.A., 2017. Struktur Histologi Hati Mencit (Mus musculus L.) setelah Pemberian Ekstrak Daun Ekor Naga (Rhapidhophora pinnata Schott). Jurnal Simbiosis, 5(2), pp.43–46.
Prior, R.L., 2003. Fruits And Vegetables in The Prevention of Cellular Oxidative Damage. The American Journal of Clinical Nutrition, 78(3), pp.570–578.
Sanger, G., Kaseger, B.E., Rarung, L.K. & Damongilala, L., 2018. Potensi Beberapa Jenis Rumput Laut sebagai Bahan Pangan Fungsional, Sumber Pigmen, dan Antioksidan Alami. JPHPI, 21(2), pp.208–217.
Sari, P.K., Lintong, M. & Loho, L.L., 2015. Efek Pemberian Anabolik Androgenik Steroid Injeksi Dosis Rendah dan Tinggi terhadap Gambaran Histopatologi Hati dan Otot Rangka Tikus Wistar (Rattus novergicus). Jurnal e-Biomedik (eBm), 3(1), pp.501–509.
Setiawati, M.D., Indahwati, L. & Khotimah, H., 2020. Pengaruh Pemberian Vitamin C dan Vitamin E terhadap Lama Siklus Estrus Tikus (Rattus norvegicus) Galur Wistar Betina yang Diberi Rhodamin B. Journal of Issues in Midwifery, 3(3), pp.80–87.
Sijid, S.A., Muthiadin, C., Zulkarnain, Hidayat, A.S. & Amelia, R.R., 2020. Pengaruh Pemberian Tuak terhadap Gambaran Histopatologi Hati Mencit (Mus musculus) ICR Jantan. Jurnal Pendidikan Matematika dan Ipa, 11(2), pp.193–205.
Sulaiman, Y., Aman, I.G.M. & Pangkahila, W., 2017. Pemberian Bisphenol-A (BPA) Oral Menurunkan Kadar Superoksida Dismutase dan Meningkatkan Kadar F2-isoprostan pada Tikus Putih (Rattus norvegicus) Jantan Galur Sprague Dawley. Jurnal Biomedik, 9(3), pp.152–158.
Tatukude, P., Loho, L. & Lintong, P., 2014. Gambaran Histopatologi Hati Mencit Swiss yang Diberi Air Rebusan Sarang Semut (Mymercodia Pendans) Paska Induksi dengan Carbon Tetrachlorida (CCl4). Jurnal e-Biomedik (eBM), 2(2), pp.459–466.
Utomo, Y., Hidayat, A., Dafip, M. & Sasi, F., 2012. Studi Histopatologi Hati Mencit (Mus Musculus L.) yang Diinduksi Pemanis Buatan. Jurnal MIPA, 35(2), pp.122–129.
Vaya, J. & Aviram, M., 2001. Nutritional Antioxidants: Mechanisms of Action, Analyses of Activities. Current Medicinal Chemistry–Immunology, Endocrine & Metabolic Agents, 1(1), pp.99–117.
Wicaksono, H.S., Narayani, I. & Setyawati, I., 2015. Struktur Hati Mencit (Mus Musculus L.) setelah Pemberian Ekstrak Daun Kaliandra Merah (Calliandra colothyrsus Meissn.). Jurnal Simbiosis III, 1(1), pp.258–268.
Published
2022-06-27
How to Cite
SAFITRI, Nuridha Audinia; ATANG, Atang; WIBOWO, Eko Setio. Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami. BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 4, n. 1, p. 46-52, june 2022. ISSN 2714-8564. Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/4606>. Date accessed: 01 feb. 2023. doi: https://doi.org/10.20884/1.bioe.2022.4.1.4606.
Section
Articles