Potensi Ekstrak Etil Asetat Coprinus comatus terhadap Kadar Ureum dan Kreatinin pada Tikus Putih Model Diabetes

  • Anik Laeli Perdanawati Universitas Jenderal Soedirman
  • Nuniek Ina Ratnaningtyas Universitas Jenderal Soedirman
  • Hernayanti Hernayanti Universitas Jenderal Soedirman

Abstract

Coprinus comatus or chicken drumstick mushrooms has potential as antioxidant and antidiabetic. Hyperglycemia in people with diabetes mellitus causes an increase in Reactive Oxygen Species (ROS). Pancreatic β cells have less antioxidants than other organs. This causes oxidative stress which triggers a chain reaction of lipid peroxidation which damages the kidneys and disturbs the glomerular filtration rate. Impaired glomerular filtration rate is characterized by increased levels of urea and creatinine. Flavonoids in C. comatus are able to donate H + and stop lipid peroxidation reactions in the kidneys, so that urea and creatinine levels decrease.This study aims to determine the effect of C. comatus ethyl acetate extract on blood urea and creatinine levels in diabetic rats and to determine the effective dose of C. comatus ethyl acetate extract on blood urea and creatinine levels in diabetic rats.


This study used an experimental method based on a completely randomized design (CRD). The data obtained from the measurement of urea and creatinine levels were analyzed using the one way Anova statistical test at the 95% confidence level and followed by Duncan's test at an error rate of 5%. The results of this study indicated that the ethyl acetate extract of C. comatus affected the blood urea and creatinine levels of diabetic rats. C. comatus extract at a dose of 500 mg/kgBW is an effective dose that has an effect on reducing levels of blood urea and creatinine in the amount of  16,66 ± 0,00 mg/dL dan 0,40 ± 0,12 mg/dL.

Author Biographies

Nuniek Ina Ratnaningtyas, Universitas Jenderal Soedirman

Faculty of Biology

Hernayanti Hernayanti, Universitas Jenderal Soedirman

Faculty of Biology

References

Akmal, M., Adam, M., Toras, M., Lubis, T.M., 2015. Pengaruh pemberian ekstrak daun pegagan (Centella asiatica (L.) Urban) terhadap konsentrasi testosteron pada tikus putih jantan (Rattus norvegicus). Jurnal Medika Veterinaria 9(1), pp.40-43.
Aldi, A. S. P., Kalsum, U., Fatmawati, 2019. Pengaruh suplementasi besi (fe) dosis tinggi terhadap kondisi sel beta pankreas pada tikus putih (Rattus norvegicus) strain wistar bunting. Journal Of Issues In Midwifery 3(1), pp.20–25.
Arrosyid, M., Choiril, H.M., 2019. Pengaruh pemberian campuran bee pollen, serbuk rimpang kencur, serbuk rimpang kunyit, biji pinang dan daun sirih terhadap volume urin pada tikus wistar pasca paparan streptozotocin (STZ). MOTORIK Jurnal Ilmu Kesehatan 14(1), pp.18-29.
Dai, K.L., Hidayah, F. K., Triliana, R., 2020. Hubungan kadar glukosa terhadap perubahan kadar asam urat, ureum, dan kreatinin serum penderita diabetes melitus tipe 2 di malang raya. Jurnal Bio Komplementer Medicine 7(2), pp.1-12.
Defriana, D., Fridayanti, A., Rijai, L., 2015. Efek Ekstrak tanduk rusa sambar (Cervusuni color) terhadap kadar ureum dan kreatinin tikus putih (Rattus novergicus). Jurnal Sains dan Kesehatan 1(2), pp.51–55.
Husen, F., 2019. Ekstrak etil asetat coprinus comatus sebagai antidiabetik dan antioksidan tikus wistar (Rattus norvegicus) hiperglikemik. Tesis. Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto.
Indrawati, S., Yuliet, Y., Ihwan, I., 2015. Efek antidiabetes ekstrak air kulit buah pisang ambon (Musa paradisiaca L.) terhadap mencit (Mus musculus) model hiperglikemia. Galenika Journal of Pharmacy 1(2), pp.133-140.
Khan.R., Ali, R., Khan, Z., Shah, S., Nawab, Z., 2012. Cinnamon on the functions of liver and kidney in type 2 diabetic individuals. Pakistan Journal of Life and Social Sciences 8(2), pp.145-149.
Kumar, S., Pandey, A.K., 2013. Chemistry and biological activities of flavonoids: an overview. The scientific world journal 1(1), pp.1-16.
Lung, J. K. S., Destiani, D. P., 2018. Uji aktivitas antioksidan vitamin a, c, e dengan metode DPPH. Farmaka 15(1), pp.53–62.
Malole, M. B. M. & Pramono, C. S. U., 1989. Pengantar Hewan-hewan Percobaan di Laboratorium. Pusat Antar Universitas Bioteknologi IPB, Bogor.
Mongi, R., Simbala, H.E., de Queljoe, E., 2019. Uji Aktivitas penurunan kadar gula darah ekstrak etanol daun pinang yaki (arecavestiaria) terhadap tikus putih jantan Galur Wistar (Rattus norvegicus) yang diinduksi aloksan. Pharmacon 8(2), pp.449-456.
Mustika, A., Indrawati, R., Sari, G.M., 2017. Efek ekstrak daun singawalang (petiveriaalliacea) dalam menurunkan kadar glukosa darah melalui peningkatan ekspresi ampk-α1 pada tikus model diabetes mellitus. Jurnal Farmasi Klinik Indonesia 6(1), pp.22-31.
Nawfa, R., Purnomo, A.S., 2016. Pengaruh tongkol jagung sebagai media pertumbuhan alternatif jamur tiram putih (Pleurotus ostreatus) terhadap aktivitas antimikroba. Jurnal Sains dan Seni ITS, 5(1), pp.57-60.
Prameswari, O.M., Widjanarko, S.B., 2014. Uji efek ekstrak air daun pandan wangi terhadap penurunan kadar glukosa darah dan histopatologi tikus diabetes mellitus. Jurnal Pangan dan agroindustri 2(2), pp.16-27.
Radji, M., Sumiati, A., Rachmayani, R., Elya, B., 2011. Isolation of fungal endophytes from garcinia mangostana and their antibacterial activity. African Journal of Biotechnology 10(1), pp.103 – 107.
Ratnaningtyas, N.I., Ekowati, N., Sukmawati, D., Widianti, H., 2019. Chicken drumstick mushroom (Coprinus comatus) ethanol extract exerts a hypoglycaemic effect in the rattus norvegicus model of diabetes. Biocatalysis and Agricultural Biotechnology, 19(1), pp.1-4.
Retnowati, A., Rugayah, J. S. R., Arifiani, D., 2019. Status Keanekaragaman Hayati Indonesia: Kekayaan Jenis Tumbuhan dan Jamur Indonesia. LIPI Press, Jakarta.
Sasmita, F.W., Susetyarini, E., Husamah, H. & Pantiwati, Y., 2017. efek ekstrak daun kembang bulan (Tithonia diversifolia) terhadap kadar glukosa darah tikus wistar (Rattus novergicus) yang diinduksi alloxan. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal 34(1), pp.22-31.
Sharma, B., Salunke, R., Balomajumder, C., Daniel, S., Roy, P., 2009. Anti-diabetic potential of alkaloid-rich fraction from capparis deciduas on diabetic mice. Journal Ethnopharmacol, 127, pp.457 – 462.
Simatupang, R., 2020. Pedoman Diet Diabetes Mellitus. Yayasan Pendidikan dan Sosial Indonesia Maju, Serang.
Song, J., Kwon, O., Chen, S., Daruwala, R., Eck, P., Park, J. B., Levine, M., 2002. Flavonoid inhibition of sodium-dependent vitamin c transporter 1 (SVCT1) and glucose transporter isoform 2 (GLUT2), intestinal transporters for vitamin c and glucose. Journal of Biological Chemistry 277(18), pp.15252-15260.
Szkudelski, T., 2001. The mechanism of alloxan and streptozotocin action in b cells of therat pancreas. Physio Res 50(6), pp.537-546.
Tandi, J., Muttaqin, H.K., Handayani, K.R., Mulyani, S. & Patala, R., 2020. Uji potensi metabolit sekunder ekstrak kulit buah petai (Parkiaspeciosa hassk) terhadap kadar kreatinin dan ureum tikus secara spektrofotometri uv-vis. KOVALEN: Jurnal Riset Kimia 6(2), pp.143–151.
Tandi, J., Wulandari, A., Asrifa, A., 2017. Efek ekstrak etanol daun gendola merah (Basella alba L.) terhadap kadar kreatinin, ureum dan deskripsi histologis tubulus ginjal tikus putih jantan (Rattus norvegicus) diabetes yang diinduksi streptozotocin. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal) 3(2), pp.93-102.
Tatukude, R.L., Loho, L., Lintong, P. M., 2014. Gambaran histopatologi hati tikus wistar yang diberikan boraks. eBiomedik, 2(3), pp.1-7.
Tesanovic, K., Pejin, B., Sibul, F., Matavulj, M., Raseta, M., Janjusevic, L., Karaman, M., 2016. A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum. Journal of Food Science and Technology 54(2), pp.430–438.
Tuldjannah, M., Tadjio, Y.K., Tandi, J., 2018. Efek nefroprotektif ekstrak daun gedi merah terhadap kadar kreatinin atau ureum tikus putih jantan diinduksi etilenglikol. Farmakologika: Jurnal Farmasi, 15(2), pp.160-167.
Wati, A., Kosman, R., Lizikri, A., 2014. perbandingan efektivitas hipoglikemik obat metformin paten dan generik berlogo berdasarkan penurunan kadar glukosa darah mencit (Mus musculus) jantan yang diinduksi aloksan. As-Syifaa Jurnal Farmasi 6(1), pp.91-97.
Widyastuti, N. Teguh, B., Reni, G., Henky, I., Priyo, W., Donowati., 2011. Analisa kandunganbeta-glukan larut air dan larut alkali daritubuh buah jamur tiram (Pleurotusostreatus) dan shiitake (Lentinus edodes). Jurnal Sains dan Teknologi Indonesia 13(3), pp.182-191.
Wild, S., Roglic, G., Green, A., Sicree, R., King, H., 2004. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 27(5), pp.1047–1053.
Published
2022-05-10
How to Cite
PERDANAWATI, Anik Laeli; RATNANINGTYAS, Nuniek Ina; HERNAYANTI, Hernayanti. Potensi Ekstrak Etil Asetat Coprinus comatus terhadap Kadar Ureum dan Kreatinin pada Tikus Putih Model Diabetes. BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 3, n. 3, p. 132-141, may 2022. ISSN 2714-8564. Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/4239>. Date accessed: 28 mar. 2024. doi: https://doi.org/10.20884/1.bioe.2021.3.3.4239.
Section
Articles