Primordial Germ Cells (PGCs) Embrio Ikan Nilem (Osteochilus vittatus) pada Beberapa Tahap Perkembangan

Abstract

Primordial Germ Cells (PGCs) are gametes precursors that are important in fish reproduction. PGCs are allocated outside the gonad and migrate towards the gonad primordia during fish embryonal development. Knowledge of the allocation and migration of PGCs is important in the development of gametogenesis research models and improved aquaculture production. This research aims to determine the location of PGCs in the embryo stage of nilem fish using the in situ hybridization method with vasa germ cell markers of nilem fish. Nilem fish embryos aged 30 minutes, 60, 180 and 240 minutes after fertilization were fixed in 10% NBF then dechorionated. Next, in situ hybridization was carried out with the Nilem fish vasa germ cell marker. PGCs were found in the area around the blastomere cleavage groove in embryos aged 30 minutes, 60 minutes and detected in the blastoderm of animal pole region in embryos aged 18 minutes and 240 minutes which had entered the blastula and gastrula stages.