Effectiveness of Probiotic Lactobacillus reuteri Lozenges in Reducing Anaerobic Bacterial Population in Gingival Crevicular Fluid After Scaling and Root Planing (Study on Chronic Periodontitis Patients at RSGMP UNSOED)
Abstract
Background: Chronic periodontitis is a destructive periodontal disease that results periodontal inflammation caused by anaerobic bacteria at subgingival plaque and gingival crevicular fluid. Periodontal treatment is needed to eliminate periodontopathogenic bacteria with non-surgical treatment scaling & root planing and consume antimicrobial medicine as adjuvant therapy. Probiotics such as Lactobacillus reuteri can be used as adjuvant therapy because it can produce bacteriosin as antibacterial substances. Objectives: This study aims to determine the effectiveness of L. reuteri probiotic lozenges in reducing the population of anaerobic bacteria in the gingival crevicular fluid of chronic periodontitis patients after scaling & root planing. Methods: . This study was clinical experimental research with pre-test post-test & control group design. Gingival crevicular fluid sample got from 4 control group patients and 4 treatment group patients. Data formed by Total Plate Count (TPC) of anaerobic bacterial colonies in gingival crevicular fluid which were statistically analyzed using the Paired t-test and Unpaired t-test. Anaerobic bacterial colonies (105 CFU/mL) was decreased at 242.25 ± 10.1 in treatment group and 165.75 ± 52.6 in control group. Key findings: The result showed that there is a significant difference mean reduction number of anaerobic bacterial colonies between the treatment group and the control group (p≤0.05). Conclusions: Conclusion of this research was that scaling & root planing combine with consume probiotic Lactobacillus reuteri lozenges is effective in reduce the population of anaerobic bacteria that cause chronic periodontitis.
