Isolasi dan Uji Aktivitas Amilase pada Bakteri yang Berasal dari Hulu sungai Logawa Banyumas
Abstract
Amylase is an enzyme that is widely used in various industrial fields. Amylase-producing microorganisms, especially bacteria, are a potential source that continues to be explored to this day. This study aims to isolate and test amylase from the natural environment, such as the water of the Logawa River. The methods used in this study include sampling, bacterial isolation, purification, amylolytic activity testing, micromorphological and macromorphological characterization, and catalase testing on bacterial isolates. The results of the study obtained three selected bacterial isolates based on macromorphological characteristics. Amylolytic activity testing with 1% substrate showed that two isolates, namely HL 1-2 and HL1-3, had amylolytic indices of 0.97 and 1.38, respectively, which were indicated by the formation of a clear zone around the colony after 48 hours of incubation at 28 °C. All isolates are Gram-positive bacteria with positive catalase test results. The results of the study indicate that the Logawa River, especially in the upstream part, has the potential to be a source of amylolytic bacteria that can be bioprospected further.
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Sundari, A. S., Purwani, N. N. P., &
Kurniati, A. (2019). Isolasi dan
penentuan indeks amilolitik bakteri
dari sedimen mangrove di Wonorejo,
Surabaya. Jurnal Inovasi Pendidikan
Sains, 10(1), 38–44.
Yasir, K., Sukainah, A., & Mustarin, A.
(2024). Isolasi dan identifikasi
mikroba amilolitik selama fermentasi
spontan pisang kepok (Musa
paradisiaca). Jurnal Pendidikan
Teknologi Pertanian, 10(2), 239–248.
Zhang, L., Zhong, M., Li, X., Lu, W., & Li, J.
(2020). River bacterial community
structure and co-occurrence patterns
under the influence of different
domestic sewage types. Journal of
Environmental Management, 266 (1),
110590.
M. S. (2024). Uji aktivitas enzim
ekstraseluler isolat mikroba endofit
mangrove (Rhizophora mucronata) di
kawasan Teluk Tomini. Journal Syifa
Sciences and Clinical Research
(JSSCR), 6(3), 274–286.
Ayal, E. L. B., Fitriadi, R., Ryandini, D.,
Nurhafid, M., Riady, R. M., &
Anandasari, M. A. (2024). Screening
of amylolytic bacteria from mina padi
aquaculture in Panembangan
Village, Cilongok District, Banyumas,
Central Java. Journal of Aquaculture
& Fish Health, 13(1), 90–101.
Basitoh, Y. K., Suarsini, E., &
Prabaningtyas, S. (2018). Eksplorasi
bakteri amilolitik potensial dari Ranu
Pani, Ranu. Jurnal Ilmu Hayat, 3(2),
52–63.
Ginting, L., Wijanarka, W., & Kusdiyantini,
E. (2020). Isolasi bakteri endofit
tanaman pepaya (Carica papaya L.)
dan uji aktivitas enzim amilase.
Berkala Bioteknologi, 3(2), 1–7.
Irsyadah, N., & Santosa, S. (2024). Isolasi
dan karakterisasi bakteri amilolitik,
proteolitik dari tanah perkebunan
pepaya dengan serangan hama kutu
putih (Paracoccus marginatus) di
Kebumen. LenteraBio: Berkala Ilmiah
Biologi, 13(1), 86–92.
Istia'nah, D., Utami, U., & Barizi, A. (2020).
Karakterisasi enzim amilase dari
bakteri Bacillus megaterium pada
variasi suhu, pH dan konsentrasi
substrat. Jurnal Riset Biologi dan
Aplikasinya, 2(1), 11–17.
Jumardin, J., Fathurrahman, F., Kadekoh,
I., & Ete, A. (2018). Eksplorasi
mikroba epifit, endofit dan rizosfer
dari berbagai sumber padi gogo di
Kecamatan Kulawi Kabupaten Sigi.
Jurnal Agrotech, 8(2), 73–78.
Klinfoong, R., Thummakasorn, C.,
Ungwiwatkul, S., Boontanom, P., &
Chantarasiri, A. (2022). Diversity and
activity of amylase-producing
bacteria isolated from mangrove soil
in Thailand. Biodiversitas Journal of
Biological Diversity, 23(10), 5519–
5531.
Laili, N. H., Abida, I. W., & Junaedi, A. S.
(2022). Nilai total plate count (TPC)
dan jumlah jenis bakteri air limbah
cucian garam (bittern) dari tambak
garam Desa Banyuajuh Kecamatan
Kamal Kabupaten
Bangkalan. Juvenil: Jurnal Ilmiah
Kelautan dan Perikanan, 3(1), 26-31.
Ningtyas, N., Mubarik, N. R., &
Rahayuningsih, M. (2023).
Penapisan dan karakterisasi amilase
dari bakteri asal ekoenzim. Jurnal Ilmu Pertanian Indonesia, 28(3),
441–448.
Nisa, I. K., Sitoresmi, P., Lukiati, B.,
Saptawati, R. T., & Rodiansyah, A.
(2021). The potential of amylase
enzyme activity against bacteria
isolated from several lakes in East
Java, Indonesia. Biodiversitas
Journal of Biological Diversity, 22(1),
42–49.
Nisa, S. K. (2022). Isolasi dan Penapisan
Bakteri Amilolitik dari Air dan
Sedimen Tambak Udang Vaname
(Litopenaeus vannamei) di Desa
Bunton, Kecamatan Adipala,
Kabupaten Cilacap
[Skripsi]. Universitas Jenderal
Soedirman.
Pricilia, S., Astuti, W., & Marliana, E.
(2018). Skrining bakteri endofit
penghasil amilase, lipase dan
protease dari daun Macaranga
hullettii King ex Hook.f. Jurnal
Atomik, 3(2), 102–105.
Ratu, L. I. N., Seumahu, C. A., & Killay, A.
(2023). Biodegradation test of
polluted river caused by domestic
wastewater using indigenous
bacteria in the Way Tomu watershed,
Ambon City. Biosaintifika: Journal of
Biology & Biology Education, 15(1),
48–59.
Rori, C. A., Kandou, F. E. F., & Tangapo, A.
M. (2020). Aktivitas enzim
ekstraseluler dari bakteri endofit
tumbuhan mangrove Avicennia
marina. Jurnal Bios Logos, 10(2), 48–
55.
Safrida, Y. D., Yulvizar, C., & Devira, C. N.
(2012). Isolation and characterization
of potential probiotics bacteria in
mackerel fish (Rastrelliger sp.).
Depik, 1(3), 200-203.
Saryono, S., Devi, S., Nugroho, T. T.,
Fadhila, W. F., Lorenita, L., Nasution,
F. S., & Suraya, N. (2023). Amylase
enzyme production with variation of
carbon sources and molecular
identification of thermophilic fungus
Aspergillus sp. LBKURCC304 from
Bukik Gadang, West Sumatra,
Indonesia. Biodiversitas Journal of
Biological Diversity, 24(2), 1200–
1205.
Satwika, T. D., Yulianti, D. M., & Hikam, A.
R. (2021). Karakteristik dan potensi
enzimatis bakteri asal tanah sampah
dapur dan kotoran ternak sebagai
kandidat agen biodegradasi sampah
organik. Al-Hayat: Journal of Biology
and Applied Biology, 4(1), 11-18.
Silaban, S., & Simamora, P. (2018). Isolasi
dan karakterisasi bakteri penghasil
amilase dari sampel air tawar Danau
Toba. EduChemia: Jurnal Kimia Dan
Pendidikan, 3(2), 222-231.
Silitonga, L. R., Nursyirwani, N., & Effendi,
I. (2019). Isolation, identification and
sensitivity of amilolitic bacteria from
mangrove ecosystem sediment in
purnama marine station Dumai on the pathogenic bacteria. Asian Journal of
Aquatic Sciences, 2(3), 257-266.
Sundari, A. S., Purwani, N. N. P., &
Kurniati, A. (2019). Isolasi dan
penentuan indeks amilolitik bakteri
dari sedimen mangrove di Wonorejo,
Surabaya. Jurnal Inovasi Pendidikan
Sains, 10(1), 38–44.
Yasir, K., Sukainah, A., & Mustarin, A.
(2024). Isolasi dan identifikasi
mikroba amilolitik selama fermentasi
spontan pisang kepok (Musa
paradisiaca). Jurnal Pendidikan
Teknologi Pertanian, 10(2), 239–248.
Zhang, L., Zhong, M., Li, X., Lu, W., & Li, J.
(2020). River bacterial community
structure and co-occurrence patterns
under the influence of different
domestic sewage types. Journal of
Environmental Management, 266 (1),
110590.
Published
2025-09-30
How to Cite
AL SAQAJ, Adinda Salwa Amellia Putri et al.
Isolasi dan Uji Aktivitas Amilase pada Bakteri yang Berasal dari Hulu sungai Logawa Banyumas.
MAIYAH, [S.l.], v. 4, n. 3, p. 168-178, sep. 2025.
ISSN 2963-4091.
Available at: <https://jos.unsoed.ac.id/index.php/maiyah/article/view/16805>. Date accessed: 25 feb. 2026.
doi: https://doi.org/10.20884/1.maiyah.2025.4.3.16805.
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Articles
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