Peran Bacillus amyloliquefaciens dalam Bioremediasi Limbah Industri Pulp dan Kertas
##plugins.pubIds.doi.readerDisplayName##
https://doi.org/10.20884/1.maiyah.2026.5.1.16426
Abstrak
Industri pulp dan kertas menghasilkan limbah cair dalam jumlah besar yang mengandung senyawa organik kompleks seperti lignin, zat pewarna, dan senyawa fenolik, yang sulit terurai secara alami dan berbahaya bagi lingkungan. Pendekatan biologis seperti bioremediasi menggunakan mikroorganisme ramah lingkungan kini menjadi alternatif yang menjanjikan. Bacillus amyloliquefaciens, bakteri Gram-positif yang mampu menghasilkan berbagai enzim seperti dye-decolorizing peroxidase (DyP), laccase, dan ligninase, terbukti efektif dalam mendegradasi senyawa-senyawa pencemar tersebut. Selain itu, bakteri ini juga memproduksi biosurfaktan yang meningkatkan kelarutan senyawa hidrofobik dan mempercepat proses degradasi. Berbagai studi in vitro menunjukkan efisiensi tinggi B. amyloliquefaciens dalam menurunkan nilai warna, COD, dan lignin dalam limbah pulp dan kertas. Aplikasi skala pilot menunjukkan potensi penggunaannya dalam sistem pengolahan limbah industri. Hasil ini menunjukkan bahwa B. amyloliquefaciens merupakan agen bioremediasi yang menjanjikan untuk pengelolaan limbah industri yang lebih berkelanjutan.
Referensi
Balhara, M., Ruhil, S., Dhankhar, S., & K. Chhillar, A. (2011). Bioactive Compounds Hold Up- Bacillus amyloliquefaciens as a Potent Bio-Control Agent. The Natural Products Journale, 1(1), 20–28. https://doi.org/10.2174/2210315511101010020
Beladjal, L., Gheysens, T., Clegg, J. S., Amar, M., & Mertens, J. (2018). Life from the ashes: survival of dry bacterial spores after very high temperature exposure. Extremophiles, 22(5), 751–759. https://doi.org/10.1007/s00792-018-1035-6
Budischowsky, D., Zwirchmayr, N. S., Hosoya, T., Bacher, M., Hettegger, H., Potthast, A., & Rosenau, T. (2021). Degradation of cellulosic key chromophores by ozone: a mechanistic and kinetic study. Cellulose, 28(10), 6051–6071. https://doi.org/10.1007/s10570-021-03909-4
Datta, D., Ghosh, S., Kumar, S., Gangola, S., Majumdar, B., Saha, R., … Kar, G. (2024). Microbial biosurfactants: Multifarious applications in sustainable agriculture. Microbiological Research, 279, 127551. https://doi.org/10.1016/j.micres.2023.127551
Deivy Andhika Permata, Anwar Kasim, Alfi Asben, & Yusniwati. (2021). Delignification of Lignocellulosic Biomass. World Journal of Advanced Research and Reviews, 12(2), 462–469. https://doi.org/10.30574/wjarr.2021.12.2.0618
Diez, M. C., Llafquen, C., Fincheira, P., Lamilla, C., Briceño, G., & Schalchli, H. (2022). Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications. Microorganisms, 10(10), 1892. https://doi.org/10.3390/microorganisms10101892
Du, H., Yao, W., Kulyar, M. F.-A., Ding, Y., Zhu, H., Pan, H., … Li, J. (2022). Effects of Bacillus amyloliquefaciens TL106 Isolated from Tibetan Pigs on Probiotic Potential and Intestinal Microbes in Weaned Piglets. Microbiology Spectrum, 10(1). https://doi.org/10.1128/spectrum.01205-21
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023a). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023b). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Guo, D., Yuan, C., Luo, Y., Chen, Y., Lu, M., Chen, G., … An, D. (2019, July 14). Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco. https://doi.org/10.1101/700757
Lacorte, S. (2003). Organic compounds in paper-mill process waters and effluents. TrAC Trends in Analytical Chemistry, 22(10), 725–737. https://doi.org/10.1016/S0165-9936(03)01009-4
Lal, K., & Garg, A. (2019). Effectiveness of synthesized aluminum and iron based inorganic polymer coagulants for pulping wastewater treatment. Journal of Environmental Chemical Engineering, 7(5), 103204. https://doi.org/10.1016/j.jece.2019.103204
Liu, X., John Martin, J. J., Li, X., Zhou, L., Li, R., Li, Q., … Cao, H. (2025). Optimization of the fermentation culture conditions of Bacillus amyloliquefaciens ck-05 using response surface methodology. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1552645
Luo, L., Zhao, C., Wang, E., Raza, A., & Yin, C. (2022). Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms. Microbiological Research, 259, 127016. https://doi.org/10.1016/j.micres.2022.127016
Mandeep, Gupta, G. K., Liu, H., & Shukla, P. (2019, December 1). Pulp and paper industry–based pollutants, their health hazards and environmental risks. Current Opinion in Environmental Science and Health, Vol. 12, pp. 48–56. Elsevier B.V. https://doi.org/10.1016/j.coesh.2019.09.010
Mei, J., Shen, X., Gang, L., Xu, H., Wu, F., & Sheng, L. (2020). A novel lignin degradation bacteria-Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently. Bioresource Technology, 310,123445. https://doi.org/10.1016/j.biortech.2020.123445
Ngalimat, M. S., Yahaya, R. S. R., Baharudin, M. M. A., Yaminudin, S. Mohd., Karim, M., Ahmad, S. A., & Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9(3), 614. https://doi.org/10.3390/microorganisms9030614
Palacios-Rodriguez, A. P., Espinoza-Culupú, A., Durán, Y., & Sánchez-Rojas, T. (2024). Antimicrobial Activity of Bacillus amyloliquefaciens BS4 against Gram-Negative Pathogenic Bacteria. Antibiotics, 13(4), 304. https://doi.org/10.3390/antibiotics13040304
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021a). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021b). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021a). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021b). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022a). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022b). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022a). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13, 1031853. https://doi.org/10.3389/fmicb.2022.1031853
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022b). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1031853
Santos, J. B. dos, Cruz, J. de O., Geraldo, L. C., Dias, E. G., Queiroz, P. R. M., Monnerat, R. G., … Blum, L. E. B. (2023). Detection and evaluation of volatile and non-volatile antifungal compounds produced by Bacillus spp. strains. Microbiological Research, 275, 127465. https://doi.org/10.1016/j.micres.2023.127465
Schalchli, H., Lamilla, C., Rubilar, O., Briceño, G., Gallardo, F., Durán, N., … Diez, M. C. (2023). Production and characterization of a biosurfactant produced by Bacillus amyloliquefaciens C11 for enhancing the solubility of pesticides. Journal of Environmental Chemical Engineering, 11(6), 111572. https://doi.org/10.1016/j.jece.2023.111572
Silva, D., Rodrigues, C. F., Lorena, C., Borges, P. T., & Martins, L. O. (2023). Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases. Biotechnology Advances, 65, 108153. https://doi.org/10.1016/j.biotechadv.2023.108153
Singh, A. K., Bilal, M., Iqbal, H. M. N., Meyer, A. S., & Raj, A. (2021). Bioremediation of lignin derivatives and phenolics in wastewater with lignin modifying enzymes: Status, opportunities and challenges. Science of The Total Environment, 777, 145988. https://doi.org/10.1016/j.scitotenv.2021.145988
Soongprasit, K., Sricharoenchaikul, V., & Atong, D. (2020). Phenol-derived products from fast pyrolysis of organosolv lignin. Energy Reports, 6, 151–167. https://doi.org/10.1016/j.egyr.2020.08.040
Wang, Q., Ou, E.-L., Wang, P.-C., Chen, Y., Wang, Z.-Y., Wang, Z.-W., … Zhang, J.-L. (2022). Bacillus amyloliquefaciens GB03 augmented tall fescue growth by regulating phytohormone and nutrient homeostasis under nitrogen deficiency. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.979883
Wang, W., Chang, J.-S., Show, K.-Y., & Lee, D.-J. (2022). Anaerobic recalcitrance in wastewater treatment: A review. Bioresource Technology, 363, 127920. https://doi.org/10.1016/j.biortech.2022.127920
Wu, W., Li, P., Huang, L., Wei, Y., Li, J., Zhang, L., & Jin, Y. (2023). The Role of Lignin Structure on Cellulase Adsorption and Enzymatic Hydrolysis. Biomass, 3(1), 96–107. https://doi.org/10.3390/biomass3010007
Yang, J., Gao, T., Zhang, Y., Wang, S., Li, H., Li, S., & Wang, S. (2019). Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens. Biotechnology Letters, 41(8–9), 1015–1021. https://doi.org/10.1007/s10529-019-02696-0
Yang, M., Li, J., Wang, S., Zhao, F., Zhang, C., Zhang, C., & Han, S. (2023). Status and trends of enzyme cocktails for efficient and ecological production in the pulp and paper industry. Journal of Cleaner Production, 418, 138196. https://doi.org/10.1016/j.jclepro.2023.138196
Balhara, M., Ruhil, S., Dhankhar, S., & K. Chhillar, A. (2011). Bioactive Compounds Hold Up- Bacillus amyloliquefaciens as a Potent Bio-Control Agent. The Natural Products Journale, 1(1), 20–28. https://doi.org/10.2174/2210315511101010020
Beladjal, L., Gheysens, T., Clegg, J. S., Amar, M., & Mertens, J. (2018). Life from the ashes: survival of dry bacterial spores after very high temperature exposure. Extremophiles, 22(5), 751–759. https://doi.org/10.1007/s00792-018-1035-6
Budischowsky, D., Zwirchmayr, N. S., Hosoya, T., Bacher, M., Hettegger, H., Potthast, A., & Rosenau, T. (2021). Degradation of cellulosic key chromophores by ozone: a mechanistic and kinetic study. Cellulose, 28(10), 6051–6071. https://doi.org/10.1007/s10570-021-03909-4
Datta, D., Ghosh, S., Kumar, S., Gangola, S., Majumdar, B., Saha, R., … Kar, G. (2024). Microbial biosurfactants: Multifarious applications in sustainable agriculture. Microbiological Research, 279, 127551. https://doi.org/10.1016/j.micres.2023.127551
Deivy Andhika Permata, Anwar Kasim, Alfi Asben, & Yusniwati. (2021). Delignification of Lignocellulosic Biomass. World Journal of Advanced Research and Reviews, 12(2), 462–469. https://doi.org/10.30574/wjarr.2021.12.2.0618
Diez, M. C., Llafquen, C., Fincheira, P., Lamilla, C., Briceño, G., & Schalchli, H. (2022). Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications. Microorganisms, 10(10), 1892. https://doi.org/10.3390/microorganisms10101892
Du, H., Yao, W., Kulyar, M. F.-A., Ding, Y., Zhu, H., Pan, H., … Li, J. (2022). Effects of Bacillus amyloliquefaciens TL106 Isolated from Tibetan Pigs on Probiotic Potential and Intestinal Microbes in Weaned Piglets. Microbiology Spectrum, 10(1). https://doi.org/10.1128/spectrum.01205-21
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023a). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023b). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Guo, D., Yuan, C., Luo, Y., Chen, Y., Lu, M., Chen, G., … An, D. (2019, July 14). Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco. https://doi.org/10.1101/700757
Lacorte, S. (2003). Organic compounds in paper-mill process waters and effluents. TrAC Trends in Analytical Chemistry, 22(10), 725–737. https://doi.org/10.1016/S0165-9936(03)01009-4
Lal, K., & Garg, A. (2019). Effectiveness of synthesized aluminum and iron based inorganic polymer coagulants for pulping wastewater treatment. Journal of Environmental Chemical Engineering, 7(5), 103204. https://doi.org/10.1016/j.jece.2019.103204
Liu, X., John Martin, J. J., Li, X., Zhou, L., Li, R., Li, Q., … Cao, H. (2025). Optimization of the fermentation culture conditions of Bacillus amyloliquefaciens ck-05 using response surface methodology. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1552645
Luo, L., Zhao, C., Wang, E., Raza, A., & Yin, C. (2022). Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms. Microbiological Research, 259, 127016. https://doi.org/10.1016/j.micres.2022.127016
Mandeep, Gupta, G. K., Liu, H., & Shukla, P. (2019, December 1). Pulp and paper industry–based pollutants, their health hazards and environmental risks. Current Opinion in Environmental Science and Health, Vol. 12, pp. 48–56. Elsevier B.V. https://doi.org/10.1016/j.coesh.2019.09.010
Mei, J., Shen, X., Gang, L., Xu, H., Wu, F., & Sheng, L. (2020). A novel lignin degradation bacteria-Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently. Bioresource Technology, 310, 123445. https://doi.org/10.1016/j.biortech.2020.123445
Ngalimat, M. S., Yahaya, R. S. R., Baharudin, M. M. A., Yaminudin, S. Mohd., Karim, M., Ahmad, S. A., & Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9(3), 614. https://doi.org/10.3390/microorganisms9030614
Palacios-Rodriguez, A. P., Espinoza-Culupú, A., Durán, Y., & Sánchez-Rojas, T. (2024). Antimicrobial Activity of Bacillus amyloliquefaciens BS4 against Gram-Negative Pathogenic Bacteria. Antibiotics, 13(4), 304. https://doi.org/10.3390/antibiotics13040304
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021a). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021b). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021a). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021b). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022a). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022b). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022a). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13, 1031853. https://doi.org/10.3389/fmicb.2022.1031853
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022b). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1031853
Santos, J. B. dos, Cruz, J. de O., Geraldo, L. C., Dias, E. G., Queiroz, P. R. M., Monnerat, R. G., … Blum, L. E. B. (2023). Detection and evaluation of volatile and non-volatile antifungal compounds produced by Bacillus spp. strains. Microbiological Research, 275, 127465. https://doi.org/10.1016/j.micres.2023.127465
Schalchli, H., Lamilla, C., Rubilar, O., Briceño, G., Gallardo, F., Durán, N., … Diez, M. C. (2023). Production and characterization of a biosurfactant produced by Bacillus amyloliquefaciens C11 for enhancing the solubility of pesticides. Journal of Environmental Chemical Engineering, 11(6), 111572. https://doi.org/10.1016/j.jece.2023.111572
Silva, D., Rodrigues, C. F., Lorena, C., Borges, P. T., & Martins, L. O. (2023). Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases. Biotechnology Advances, 65, 108153. https://doi.org/10.1016/j.biotechadv.2023.108153
Singh, A. K., Bilal, M., Iqbal, H. M. N., Meyer, A. S., & Raj, A. (2021). Bioremediation of lignin derivatives and phenolics in wastewater with lignin modifying enzymes: Status, opportunities and challenges. Science of The Total Environment, 777, 145988. https://doi.org/10.1016/j.scitotenv.2021.145988
Soongprasit, K., Sricharoenchaikul, V., & Atong, D. (2020). Phenol-derived products from fast pyrolysis of organosolv lignin. Energy Reports, 6, 151–167. https://doi.org/10.1016/j.egyr.2020.08.040
Wang, Q., Ou, E.-L., Wang, P.-C., Chen, Y., Wang, Z.-Y., Wang, Z.-W., … Zhang, J.-L. (2022). Bacillus amyloliquefaciens GB03 augmented tall fescue growth by regulating phytohormone and nutrient homeostasis under nitrogen deficiency. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.979883
Wang, W., Chang, J.-S., Show, K.-Y., & Lee, D.-J. (2022). Anaerobic recalcitrance in wastewater treatment: A review. Bioresource Technology, 363, 127920. https://doi.org/10.1016/j.biortech.2022.127920
Wu, W., Li, P., Huang, L., Wei, Y., Li, J., Zhang, L., & Jin, Y. (2023). The Role of Lignin Structure on Cellulase Adsorption and Enzymatic Hydrolysis. Biomass, 3(1), 96–107. https://doi.org/10.3390/biomass3010007
Yang, J., Gao, T., Zhang, Y., Wang, S., Li, H., Li, S., & Wang, S. (2019). Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens. Biotechnology Letters, 41(8–9), 1015–1021. https://doi.org/10.1007/s10529-019-02696-0
Yang, M., Li, J., Wang, S., Zhao, F., Zhang, C., Zhang, C., & Han, S. (2023). Status and trends of enzyme cocktails for efficient and ecological production in the pulp and paper industry. Journal of Cleaner Production, 418, 138196. https://doi.org/10.1016/j.jclepro.2023.138196
Zalila-Kolsi, I., Ben-Mahmoud, A., & Al-Barazie, R. (2023). Bacillus amyloliquefaciens: Harnessing Its Potential for Industrial, Medical, and Agricultural Applications—A Comprehensive Review. Microorganisms, 11(9), 2215. https://doi.org/10.3390/microorganisms11092215
Zheng, X., Zhao, Y., Xu, H., Fan, Q., Li, Y., Chen, L., … Hua, D. (2022). Enhancing the anaerobic digestion of papermaking black liquor with three-dimensional iron-carbon electrolysis and assessment of microbial community changes. Journal of Environmental Chemical Engineering, 10(4), 108115. https://doi.org/10.1016/j.jece.2022.108115
Beladjal, L., Gheysens, T., Clegg, J. S., Amar, M., & Mertens, J. (2018). Life from the ashes: survival of dry bacterial spores after very high temperature exposure. Extremophiles, 22(5), 751–759. https://doi.org/10.1007/s00792-018-1035-6
Budischowsky, D., Zwirchmayr, N. S., Hosoya, T., Bacher, M., Hettegger, H., Potthast, A., & Rosenau, T. (2021). Degradation of cellulosic key chromophores by ozone: a mechanistic and kinetic study. Cellulose, 28(10), 6051–6071. https://doi.org/10.1007/s10570-021-03909-4
Datta, D., Ghosh, S., Kumar, S., Gangola, S., Majumdar, B., Saha, R., … Kar, G. (2024). Microbial biosurfactants: Multifarious applications in sustainable agriculture. Microbiological Research, 279, 127551. https://doi.org/10.1016/j.micres.2023.127551
Deivy Andhika Permata, Anwar Kasim, Alfi Asben, & Yusniwati. (2021). Delignification of Lignocellulosic Biomass. World Journal of Advanced Research and Reviews, 12(2), 462–469. https://doi.org/10.30574/wjarr.2021.12.2.0618
Diez, M. C., Llafquen, C., Fincheira, P., Lamilla, C., Briceño, G., & Schalchli, H. (2022). Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications. Microorganisms, 10(10), 1892. https://doi.org/10.3390/microorganisms10101892
Du, H., Yao, W., Kulyar, M. F.-A., Ding, Y., Zhu, H., Pan, H., … Li, J. (2022). Effects of Bacillus amyloliquefaciens TL106 Isolated from Tibetan Pigs on Probiotic Potential and Intestinal Microbes in Weaned Piglets. Microbiology Spectrum, 10(1). https://doi.org/10.1128/spectrum.01205-21
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023a). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023b). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Guo, D., Yuan, C., Luo, Y., Chen, Y., Lu, M., Chen, G., … An, D. (2019, July 14). Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco. https://doi.org/10.1101/700757
Lacorte, S. (2003). Organic compounds in paper-mill process waters and effluents. TrAC Trends in Analytical Chemistry, 22(10), 725–737. https://doi.org/10.1016/S0165-9936(03)01009-4
Lal, K., & Garg, A. (2019). Effectiveness of synthesized aluminum and iron based inorganic polymer coagulants for pulping wastewater treatment. Journal of Environmental Chemical Engineering, 7(5), 103204. https://doi.org/10.1016/j.jece.2019.103204
Liu, X., John Martin, J. J., Li, X., Zhou, L., Li, R., Li, Q., … Cao, H. (2025). Optimization of the fermentation culture conditions of Bacillus amyloliquefaciens ck-05 using response surface methodology. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1552645
Luo, L., Zhao, C., Wang, E., Raza, A., & Yin, C. (2022). Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms. Microbiological Research, 259, 127016. https://doi.org/10.1016/j.micres.2022.127016
Mandeep, Gupta, G. K., Liu, H., & Shukla, P. (2019, December 1). Pulp and paper industry–based pollutants, their health hazards and environmental risks. Current Opinion in Environmental Science and Health, Vol. 12, pp. 48–56. Elsevier B.V. https://doi.org/10.1016/j.coesh.2019.09.010
Mei, J., Shen, X., Gang, L., Xu, H., Wu, F., & Sheng, L. (2020). A novel lignin degradation bacteria-Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently. Bioresource Technology, 310,123445. https://doi.org/10.1016/j.biortech.2020.123445
Ngalimat, M. S., Yahaya, R. S. R., Baharudin, M. M. A., Yaminudin, S. Mohd., Karim, M., Ahmad, S. A., & Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9(3), 614. https://doi.org/10.3390/microorganisms9030614
Palacios-Rodriguez, A. P., Espinoza-Culupú, A., Durán, Y., & Sánchez-Rojas, T. (2024). Antimicrobial Activity of Bacillus amyloliquefaciens BS4 against Gram-Negative Pathogenic Bacteria. Antibiotics, 13(4), 304. https://doi.org/10.3390/antibiotics13040304
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021a). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021b). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021a). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021b). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022a). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022b). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022a). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13, 1031853. https://doi.org/10.3389/fmicb.2022.1031853
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022b). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1031853
Santos, J. B. dos, Cruz, J. de O., Geraldo, L. C., Dias, E. G., Queiroz, P. R. M., Monnerat, R. G., … Blum, L. E. B. (2023). Detection and evaluation of volatile and non-volatile antifungal compounds produced by Bacillus spp. strains. Microbiological Research, 275, 127465. https://doi.org/10.1016/j.micres.2023.127465
Schalchli, H., Lamilla, C., Rubilar, O., Briceño, G., Gallardo, F., Durán, N., … Diez, M. C. (2023). Production and characterization of a biosurfactant produced by Bacillus amyloliquefaciens C11 for enhancing the solubility of pesticides. Journal of Environmental Chemical Engineering, 11(6), 111572. https://doi.org/10.1016/j.jece.2023.111572
Silva, D., Rodrigues, C. F., Lorena, C., Borges, P. T., & Martins, L. O. (2023). Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases. Biotechnology Advances, 65, 108153. https://doi.org/10.1016/j.biotechadv.2023.108153
Singh, A. K., Bilal, M., Iqbal, H. M. N., Meyer, A. S., & Raj, A. (2021). Bioremediation of lignin derivatives and phenolics in wastewater with lignin modifying enzymes: Status, opportunities and challenges. Science of The Total Environment, 777, 145988. https://doi.org/10.1016/j.scitotenv.2021.145988
Soongprasit, K., Sricharoenchaikul, V., & Atong, D. (2020). Phenol-derived products from fast pyrolysis of organosolv lignin. Energy Reports, 6, 151–167. https://doi.org/10.1016/j.egyr.2020.08.040
Wang, Q., Ou, E.-L., Wang, P.-C., Chen, Y., Wang, Z.-Y., Wang, Z.-W., … Zhang, J.-L. (2022). Bacillus amyloliquefaciens GB03 augmented tall fescue growth by regulating phytohormone and nutrient homeostasis under nitrogen deficiency. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.979883
Wang, W., Chang, J.-S., Show, K.-Y., & Lee, D.-J. (2022). Anaerobic recalcitrance in wastewater treatment: A review. Bioresource Technology, 363, 127920. https://doi.org/10.1016/j.biortech.2022.127920
Wu, W., Li, P., Huang, L., Wei, Y., Li, J., Zhang, L., & Jin, Y. (2023). The Role of Lignin Structure on Cellulase Adsorption and Enzymatic Hydrolysis. Biomass, 3(1), 96–107. https://doi.org/10.3390/biomass3010007
Yang, J., Gao, T., Zhang, Y., Wang, S., Li, H., Li, S., & Wang, S. (2019). Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens. Biotechnology Letters, 41(8–9), 1015–1021. https://doi.org/10.1007/s10529-019-02696-0
Yang, M., Li, J., Wang, S., Zhao, F., Zhang, C., Zhang, C., & Han, S. (2023). Status and trends of enzyme cocktails for efficient and ecological production in the pulp and paper industry. Journal of Cleaner Production, 418, 138196. https://doi.org/10.1016/j.jclepro.2023.138196
Balhara, M., Ruhil, S., Dhankhar, S., & K. Chhillar, A. (2011). Bioactive Compounds Hold Up- Bacillus amyloliquefaciens as a Potent Bio-Control Agent. The Natural Products Journale, 1(1), 20–28. https://doi.org/10.2174/2210315511101010020
Beladjal, L., Gheysens, T., Clegg, J. S., Amar, M., & Mertens, J. (2018). Life from the ashes: survival of dry bacterial spores after very high temperature exposure. Extremophiles, 22(5), 751–759. https://doi.org/10.1007/s00792-018-1035-6
Budischowsky, D., Zwirchmayr, N. S., Hosoya, T., Bacher, M., Hettegger, H., Potthast, A., & Rosenau, T. (2021). Degradation of cellulosic key chromophores by ozone: a mechanistic and kinetic study. Cellulose, 28(10), 6051–6071. https://doi.org/10.1007/s10570-021-03909-4
Datta, D., Ghosh, S., Kumar, S., Gangola, S., Majumdar, B., Saha, R., … Kar, G. (2024). Microbial biosurfactants: Multifarious applications in sustainable agriculture. Microbiological Research, 279, 127551. https://doi.org/10.1016/j.micres.2023.127551
Deivy Andhika Permata, Anwar Kasim, Alfi Asben, & Yusniwati. (2021). Delignification of Lignocellulosic Biomass. World Journal of Advanced Research and Reviews, 12(2), 462–469. https://doi.org/10.30574/wjarr.2021.12.2.0618
Diez, M. C., Llafquen, C., Fincheira, P., Lamilla, C., Briceño, G., & Schalchli, H. (2022). Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications. Microorganisms, 10(10), 1892. https://doi.org/10.3390/microorganisms10101892
Du, H., Yao, W., Kulyar, M. F.-A., Ding, Y., Zhu, H., Pan, H., … Li, J. (2022). Effects of Bacillus amyloliquefaciens TL106 Isolated from Tibetan Pigs on Probiotic Potential and Intestinal Microbes in Weaned Piglets. Microbiology Spectrum, 10(1). https://doi.org/10.1128/spectrum.01205-21
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023a). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., … Landeka Dragičević, T. (2023b). The Bacterial Degradation of Lignin—A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272
Guo, D., Yuan, C., Luo, Y., Chen, Y., Lu, M., Chen, G., … An, D. (2019, July 14). Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco. https://doi.org/10.1101/700757
Lacorte, S. (2003). Organic compounds in paper-mill process waters and effluents. TrAC Trends in Analytical Chemistry, 22(10), 725–737. https://doi.org/10.1016/S0165-9936(03)01009-4
Lal, K., & Garg, A. (2019). Effectiveness of synthesized aluminum and iron based inorganic polymer coagulants for pulping wastewater treatment. Journal of Environmental Chemical Engineering, 7(5), 103204. https://doi.org/10.1016/j.jece.2019.103204
Liu, X., John Martin, J. J., Li, X., Zhou, L., Li, R., Li, Q., … Cao, H. (2025). Optimization of the fermentation culture conditions of Bacillus amyloliquefaciens ck-05 using response surface methodology. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1552645
Luo, L., Zhao, C., Wang, E., Raza, A., & Yin, C. (2022). Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms. Microbiological Research, 259, 127016. https://doi.org/10.1016/j.micres.2022.127016
Mandeep, Gupta, G. K., Liu, H., & Shukla, P. (2019, December 1). Pulp and paper industry–based pollutants, their health hazards and environmental risks. Current Opinion in Environmental Science and Health, Vol. 12, pp. 48–56. Elsevier B.V. https://doi.org/10.1016/j.coesh.2019.09.010
Mei, J., Shen, X., Gang, L., Xu, H., Wu, F., & Sheng, L. (2020). A novel lignin degradation bacteria-Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently. Bioresource Technology, 310, 123445. https://doi.org/10.1016/j.biortech.2020.123445
Ngalimat, M. S., Yahaya, R. S. R., Baharudin, M. M. A., Yaminudin, S. Mohd., Karim, M., Ahmad, S. A., & Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9(3), 614. https://doi.org/10.3390/microorganisms9030614
Palacios-Rodriguez, A. P., Espinoza-Culupú, A., Durán, Y., & Sánchez-Rojas, T. (2024). Antimicrobial Activity of Bacillus amyloliquefaciens BS4 against Gram-Negative Pathogenic Bacteria. Antibiotics, 13(4), 304. https://doi.org/10.3390/antibiotics13040304
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021a). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Patel, K., Patel, N., Vaghamshi, N., Shah, K., Duggirala, S. M., & Dudhagara, P. (2021b). Trends and strategies in the effluent treatment of pulp and paper industries: A review highlighting reactor options. Current Research in Microbial Sciences, 2, 100077. https://doi.org/10.1016/j.crmicr.2021.100077
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021a). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Radziff, S. B. M., Ahmad, S. A., Shaharuddin, N. A., Merican, F., Kok, Y.-Y., Zulkharnain, A., … Wong, C.-Y. (2021b). Potential Application of Algae in Biodegradation of Phenol: A Review and Bibliometric Study. Plants, 10(12), 2677. https://doi.org/10.3390/plants10122677
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022a). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Huo, J., Wang, Q., Liu, Z., Li, S., Wang, S., … Li, H. (2022b). Characteristics of immobilized dye-decolorizing peroxidase from Bacillus amyloliquefaciens and application to the bioremediation of dyeing effluent. Biochemical Engineering Journal, 182, 108430. https://doi.org/10.1016/j.bej.2022.108430
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022a). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13, 1031853. https://doi.org/10.3389/fmicb.2022.1031853
Ren, J., Li, X., Zhang, W., Li, Z., Wang, Q., Li, S., … Li, H. (2022b). Evaluation of application potential of dye-decolorizing peroxidase from Bacillus amyloliquefaciens in bioremediation of paper and pulp mill effluent. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1031853
Santos, J. B. dos, Cruz, J. de O., Geraldo, L. C., Dias, E. G., Queiroz, P. R. M., Monnerat, R. G., … Blum, L. E. B. (2023). Detection and evaluation of volatile and non-volatile antifungal compounds produced by Bacillus spp. strains. Microbiological Research, 275, 127465. https://doi.org/10.1016/j.micres.2023.127465
Schalchli, H., Lamilla, C., Rubilar, O., Briceño, G., Gallardo, F., Durán, N., … Diez, M. C. (2023). Production and characterization of a biosurfactant produced by Bacillus amyloliquefaciens C11 for enhancing the solubility of pesticides. Journal of Environmental Chemical Engineering, 11(6), 111572. https://doi.org/10.1016/j.jece.2023.111572
Silva, D., Rodrigues, C. F., Lorena, C., Borges, P. T., & Martins, L. O. (2023). Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases. Biotechnology Advances, 65, 108153. https://doi.org/10.1016/j.biotechadv.2023.108153
Singh, A. K., Bilal, M., Iqbal, H. M. N., Meyer, A. S., & Raj, A. (2021). Bioremediation of lignin derivatives and phenolics in wastewater with lignin modifying enzymes: Status, opportunities and challenges. Science of The Total Environment, 777, 145988. https://doi.org/10.1016/j.scitotenv.2021.145988
Soongprasit, K., Sricharoenchaikul, V., & Atong, D. (2020). Phenol-derived products from fast pyrolysis of organosolv lignin. Energy Reports, 6, 151–167. https://doi.org/10.1016/j.egyr.2020.08.040
Wang, Q., Ou, E.-L., Wang, P.-C., Chen, Y., Wang, Z.-Y., Wang, Z.-W., … Zhang, J.-L. (2022). Bacillus amyloliquefaciens GB03 augmented tall fescue growth by regulating phytohormone and nutrient homeostasis under nitrogen deficiency. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.979883
Wang, W., Chang, J.-S., Show, K.-Y., & Lee, D.-J. (2022). Anaerobic recalcitrance in wastewater treatment: A review. Bioresource Technology, 363, 127920. https://doi.org/10.1016/j.biortech.2022.127920
Wu, W., Li, P., Huang, L., Wei, Y., Li, J., Zhang, L., & Jin, Y. (2023). The Role of Lignin Structure on Cellulase Adsorption and Enzymatic Hydrolysis. Biomass, 3(1), 96–107. https://doi.org/10.3390/biomass3010007
Yang, J., Gao, T., Zhang, Y., Wang, S., Li, H., Li, S., & Wang, S. (2019). Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens. Biotechnology Letters, 41(8–9), 1015–1021. https://doi.org/10.1007/s10529-019-02696-0
Yang, M., Li, J., Wang, S., Zhao, F., Zhang, C., Zhang, C., & Han, S. (2023). Status and trends of enzyme cocktails for efficient and ecological production in the pulp and paper industry. Journal of Cleaner Production, 418, 138196. https://doi.org/10.1016/j.jclepro.2023.138196
Zalila-Kolsi, I., Ben-Mahmoud, A., & Al-Barazie, R. (2023). Bacillus amyloliquefaciens: Harnessing Its Potential for Industrial, Medical, and Agricultural Applications—A Comprehensive Review. Microorganisms, 11(9), 2215. https://doi.org/10.3390/microorganisms11092215
Zheng, X., Zhao, Y., Xu, H., Fan, Q., Li, Y., Chen, L., … Hua, D. (2022). Enhancing the anaerobic digestion of papermaking black liquor with three-dimensional iron-carbon electrolysis and assessment of microbial community changes. Journal of Environmental Chemical Engineering, 10(4), 108115. https://doi.org/10.1016/j.jece.2022.108115
Diterbitkan
2026-03-31
##submission.howToCite##
PRASTIYO, Jery; MAEDA, Minoru; HIDAYATI, Nuning Vita.
Peran Bacillus amyloliquefaciens dalam Bioremediasi Limbah Industri Pulp dan Kertas.
MAIYAH, [S.l.], v. 5, n. 1, p. 33-44, mar. 2026.
ISSN 2963-4091.
Tersedia pada: <https://jos.unsoed.ac.id/index.php/maiyah/article/view/16426>. Tanggal Akses: 27 july 2026
doi: https://doi.org/10.20884/1.maiyah.2026.5.1.16426.
Bagian
Articles
