Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus
Abstract
The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. Streptomyces sp. SAE4034 was known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus, but the group of antibacterial compounds and their inhibitory mechanisms were not yet known. The ability of Streptomyces sp. SAE4034 in producing antioxidants is unknown yet. This research aimed to determine the classification of antibacterial compounds produced by Streptomyces sp. SAE4034, to know the ability of Streptomyces sp. SAE4034 in producing antioxidant compounds, and to observe the inhibitory mechanisms of antibacterial compounds produced by Streptomyces sp. SAE4034 against S. aureus using cell leakage method. The results of this research show that Streptomyces sp. SAE4034 produce compounds in the group of alkaloid, terpenoid, flavonoid, polyphenol. Compound with Rf values 0,40 and 0,47 which includes into polyphenol and 0,72 which includes into alkaloid group has the ability to inhibit the growth of S. aureus. Streptomyces sp. SAE4034 has a very strong antioxidant activity that can scavenge DPPH free radical with IC50 value of 32,14 ppm and has an antioxidant enzyme activity that is superoxide dismutase amount of 63,10 U/mL The inhibitory mechanism of Streptomyces sp. SAE4034 extract is through inhibition of protein membrane synthesis by the release of nucleic acid and protein material in S. aureus cells and the highest cell leakage was at 2 MIC doses.
References
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Chauhan, A. & Jindal, T., 2020. Microbiological Methods for Environment, Food and Pharmaceutical Analysis. Cham: Springer Nature.
Choma, I. & Grzelak, E. M., 2011. Bioautography Detection in Thin Layer Chromatography. Journal Chromatography, 1218, pp. 2684-2691.
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Kumar, S. R. & Rao, K. V. B., 2012. In-vitro Antimicrobial Activity of Marine Actinobacteria Against Multidrug Resistance Staphylococcus aureus. Asian Pasific Journal of Tropical Biomedicine, 2(10), pp. 787-792.
Markham, K. R., 1982. Techniques of Flavonoid Identification. London: Academic Press.
Matuszewska, A., Jaszek, M., Stefaniuk, D., Ciszewski, T. & Matuszewski, L., 2018. Anticancer, Antioxidant, and Antibacterial Activities of Low Molecular Weight Bioactive Subfractions Isolated from Cultures of Wood Degrading Fugus Cerrena unicolor. Plos One, 13(6), oo. 1-14.
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Miksusanti, Jennie, B. S. L., Ponco, B. & Trimulyadi, G., 2008. Kerusakan Dinding Sel Escherichia coli K1.1 Oleh Minyak Atsiri Temu Kunci (Kaempferia pandurata). Berita Biologi, 9(1), pp. 1-8.
Molyneux, P., 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH), For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 26(2), pp. 211-219.
Nedialkova, D. & Naidenova, M., 2005. Screening the Antimicrobial Activity of Actinomycetes Strains Isolated from Antarctica. Journal of Culture Collections, 4(1), pp. 29-35.
Nurkanto, A., Listyaningsih, F., Julistiono, H. & Agusta, A., 2010. Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate sebagai Sumber Antibiotik. Jurnal Biologi Indonesia, 6(3), pp. 325-339.
Othman, L., Sleman, A. & Massih, R. M., 2019. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Frontiers in Microbiology,10(911), pp. 1-28.
Papuc, C., Goran, G. V., Predescu, C. N., Nicorescu, V. & Stefan, G., 2017. Plant Polyphenols as Antioxidant and Antibacterial Agents for Shelf-Life Extension of Meat and Meat Products: Classification, Structures, Sources, and Action Mechanisms. Comprehensive Review in Food Science and Food Safety, 16(1), pp. 1243-1268.
Pravekar, P., Palaskar, J., Metgud, S., Maria, R. & Dutta, S., 2020. The Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC) of Silver Nanoparticles Against Staphylococcus aureus. Biomaterial Investigations in Dentistry, 7(1), pp. 105-109.
Pratiwi, R. H., Hanafi, M., Artanti, N. & Pratiwi, R. D., 2018. Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Nessia altissima. The Journal of Tropical Life Science, 8(1), pp. 37-42.
Rao, K. V. R., Mani, P., Satyanarayana, B. & Rao, T. R., 2017. Purification and Structural Elucidation of Three Bioactive Compound Isolated from Streptomyces coelicoflavus BC 01 and Their Biological Activity. Biotech , 7(24), pp. 1-12.
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Rozik, M., Sudianto, A. & Nuroyam, H., 2011. Aktivitas Enzim Superoksida Dismutase dan Enzim Protease pada Udang Windu (Penaeus monodon Fabricus) yang Diinfeksi Vibrio harveyi Pasca-Pemberian Imunostimulan OMP Vibrio alginolyticus. Berkas Penelitian Hayati Edisi Khusus,6, pp. 7-11.
Ryandini, D., Pramono, H. & Sukanto, 2018. Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria. Biosaintifika, 10(1), pp. 117-124.
Simanjuntak, P., Parwati, T., Lenni , L. E., Tamat, R. & Murwani, R, 2004. Isolasi dan Identifikasi Antioksidan dan Ekstrak Benalu Teh Isolasi Ortiana (Korth denses). Jurnal Ilmu Kefarmasian Indonesia , 5(1), pp. 19-24.
Singh, L. S., Sharma, H. & Talukdar, N. C., 2014. Production of Potent Antimicrobial Agent by Actinomycete, Streptomyces sannanensis Strain SU 118 Isolated from Phoomdi in Loktak Lake Manipur, India. BMC Microbiology, 14(278), pp. 1-14.
Sudirman, S., 2011. Aktivitas Antioksidan dan Komponen Bioaktif Kangkung Air (Ipomoea aquatic Forsk.). Skripsi. Bogor: IPB.
Sulistyo, 1971. Farmakologi dan Terapi. Yogyakarta: Penerbit EKG.
Syarifuddin, A., Sulistyani, N. & Kintoko., 2018. Aktivitas Antibiotik Isolat Bakteri Kp13 dan Analisa Kebocoran Sel Bakteri Escherichia coli. Jurnal Ilmu Kefarmasian Indonesia, 16(2), pp. 137-144.
Taecowisan, T., Chaisaeng, S. & Phutdhawong, W. S., 2017. Antibacterial, Antioxidant, and Anticancer Activities of Biphenyls from Streptomyces sp. BO-07: an Endophyte in Boesenbergia rotunda (L.) Mansf A. Food and Agricultural Immunology, 28(6), pp. 1330-1346.
Tan , L. T. H., Ser, H. L., Yin, W. F., Chan, K. G., Lee, L. H. & Goh, B. H., 2015. Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated From Malaysia Mangrove Soil. Frontier in Microbiology, 6(1316), pp. 1-12.
Tan, L. T. H., Chan, K. G., Chan, C. K., Khan, T. M., Lee, L. H. & Goh, B. H., 2018. Antioxidative Potential of a Streptomyces sp. MUM292 Isolated from Mangrove Soil. Hindawi BioMed Research International, 1(1), pp. 1-13.
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Tortora, G. J., Funke, B. R. & Christine, L. C., 2002. Microbiology: An Introduction. San Fransisco: Pearson Education Inc.
Tyas, S. H., Syarifuddin, A. & Septianingrum, N. M., 2021. Optimasi Waktu Produksi Antibakteri Isolat Actinomycetes (Isolat TE 235) terhadap Aktivitas Antibakteri pada Escherichia coli dan Staphylococcus aureus. Jurnal Farmasi Sains dan Praktis, 7(1), pp. 15-24.
Wagner, H. & Bladt, S., 1996. Plant Drug Analysis: A thin Layer Chromatography Atlas. Berlin: Springer-Verlag.
Wall, P. E., 2007. Thin-Layer Chromatography: A Modern Practical Approach. United Kingdom: Royal Society of Chemistry.
Wardana, R. S., Ryandini, D. & Oedjijono, O., 2017. Antibacterial Capacity of Streptomyces Isolate from a Mangrove Plant Rhizosphere Avicennia marina. Scripta Biologica, 4(2), pp. 131-134.
Wulandari, L., 2011. Kromatografi Lapis Tipis. Jember: PT. Taman Kampus Presindo.
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Andrieyani, Hanapi, A., Fasya, A. G. & Hasanah, H., 2015. Identifikasi Senyawa Flavonoid dan Efek Terapi Ekstrak Etanol 70% Umbi Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Kadar Glukosa Darah dan Aktifitas SOD (Superoksida dismutase) Jantung Tikus yang Diinduksi Aloksan. Alchemy, 4(1), pp. 73-78.
Ariavianti, E., 2016. Uji Aktivitas Antibakteri dan Antioksidn dari Ekstrak Metanol, Fraksi n-Heksana, dan Fraksi Etil Asetat Daun Vitex trifolia L. Asal Lombok. Skripsi. Jakarta: Universitas Negeri Jakarta.
Arnanda, Q. P. & Nuwarda, R. F., 2019. Penggunaan Radiofarmaka Teknesium-99M dari Senyawa Glutation dan Senyawa Flavonoid sebagai Deteksi Dini Radikal Bebas Pemicu Kanker. Farmaka, 17(2), pp. 236-243.
Artini, P. E. U. D., Astuti, K. W., & Warditiani, N. K. 2013. Uji Fitokimia Ekstrak Etil Asetat Rimpang Bangle (Zingiber purpureum Roxb). Jurnal Farmasi Udayana, 2(4), pp. 6-12.
Begum, A., Rao, K. N. V., Dutt, R., Giri, K., Sindhu, K., Fathima, U., Gowthami, G., Kumar, J. V., Naveen, N. & Shaffath, S., 2017. Chemical Screening and Thin Layer Chromatography of Indian Asparagus officinalis Linn. International Journal of Advanced Research, 5(4), pp. 1520-1528.
Bele, A. A. & Khale, A., 2011. An Overview on Thin Layer Chromatography. International Journal of Pharmaceutical Sciences and Research, 2(2), pp. 256-267.
Cappucino, J. G. & Sherman, N., 2014. Manual Laboratorium Mikrobiologi. 8th Edition. Jakarta: EGC.
Chauhan, A. & Jindal, T., 2020. Microbiological Methods for Environment, Food and Pharmaceutical Analysis. Cham: Springer Nature.
Choma, I. & Grzelak, E. M., 2011. Bioautography Detection in Thin Layer Chromatography. Journal Chromatography, 1218, pp. 2684-2691.
Choma, I. M. & Jesionek, W., 2015. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography, 2, pp. 225-238.
Fajriaty, I., Hariyanto, I. H., Andres. & Setyaningrum, R., 2018. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis dari Ekstrak Etanol Daun Bintagur (Calophyllum soulattri Burm. F.). Jurnal Pendidikan Informatika dan Sains, 7(1), pp. 55-67.
Gnanamani, A., Hariharan, P. & Paul-Satyaseela, M., 2017. Staphylococcus aureus: Overview of Bacteriology, Clinical Diseases, Epidemiology, Antibiotic Resistance and Therapeutic Approach, in Frontiers in Staphylococcus aureus. InTech.
Goldman, E. & Green, L. H., 2009. Practical Handbook of Microbiology. 2nd edition. New York: CRC Press
Harborne, J. B., 1987. Metode Fitokimia: Penuntun Cara modern Menganalisis Tumbuhan. edisi 2. Bandung: ITB.
Hasani, A., Kariminik, A. & Issazadeh, K., 2014. Streptomycetes: Characteristics and Their Antimicrobial Activities. International Journal of Advanced Biological and Biomedical Research, 2(1), pp. 63-75.
Ikhlas, N., 2013. Uji Aktivitas Antioksidan Ekstrak Herba Kemangi (Ocinum americanum Linn.) dengan Metode DPPH (2, 2-Difenil -1- Pikrilhidrazil). Skripsi. Jakarta: UIN Syarif Hidayatullah.
Jafar, W., Masriany. & Sukmawaty, E., 2020. Uji Fitokimia Ekstrak Etanol Bunga Pohon Hujan (Spathodea campanulata) Secara In Vitro. Prosiding Seminar Nasional Biotik, 1(1), pp. 328-334.
Kanth, B. A., Jnawali, H. N., Niraula, P. & Sohng, J. K., 2011. Superoxide dismutase (SOD) Genes in Streptomyces peucetius: Effects of SODs on Secondary Metabolites Production. Elsevier, 166(5), pp. 391-402.
Kemung, H. M. Tan, L. T. H., Chan, K. G., Ser, H. L, Law, J. W. F., Lee, L. H. & Goh, B. H., 2020. Antioxidant Activities of Streptomyces sp. Strain MUSC 14 from Mangrove Forest Soil in Malaysia. Hindawi BioMed Research International, 1(1), pp. 1-12.
Kumalasari, E. & Sulistyani, N. 2011. Aktivitas Antifungi Ekstrak Etanol Batang Binahong (Anredera cordifolia (Tenore) Steen.) terhadap Candida albicans serta Skrining Fitokimia. Jurnal Ilmiah Kefarmasian, 1(2), pp. 51-62.
Kumar, S. R. & Rao, K. V. B., 2012. In-vitro Antimicrobial Activity of Marine Actinobacteria Against Multidrug Resistance Staphylococcus aureus. Asian Pasific Journal of Tropical Biomedicine, 2(10), pp. 787-792.
Markham, K. R., 1982. Techniques of Flavonoid Identification. London: Academic Press.
Matuszewska, A., Jaszek, M., Stefaniuk, D., Ciszewski, T. & Matuszewski, L., 2018. Anticancer, Antioxidant, and Antibacterial Activities of Low Molecular Weight Bioactive Subfractions Isolated from Cultures of Wood Degrading Fugus Cerrena unicolor. Plos One, 13(6), oo. 1-14.
Merck., 2010. Microbiology Manual. 12th Edition. Darmstadt: Merck KGaA.
Miksusanti, Jennie, B. S. L., Ponco, B. & Trimulyadi, G., 2008. Kerusakan Dinding Sel Escherichia coli K1.1 Oleh Minyak Atsiri Temu Kunci (Kaempferia pandurata). Berita Biologi, 9(1), pp. 1-8.
Molyneux, P., 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH), For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 26(2), pp. 211-219.
Nedialkova, D. & Naidenova, M., 2005. Screening the Antimicrobial Activity of Actinomycetes Strains Isolated from Antarctica. Journal of Culture Collections, 4(1), pp. 29-35.
Nurkanto, A., Listyaningsih, F., Julistiono, H. & Agusta, A., 2010. Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate sebagai Sumber Antibiotik. Jurnal Biologi Indonesia, 6(3), pp. 325-339.
Othman, L., Sleman, A. & Massih, R. M., 2019. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Frontiers in Microbiology,10(911), pp. 1-28.
Papuc, C., Goran, G. V., Predescu, C. N., Nicorescu, V. & Stefan, G., 2017. Plant Polyphenols as Antioxidant and Antibacterial Agents for Shelf-Life Extension of Meat and Meat Products: Classification, Structures, Sources, and Action Mechanisms. Comprehensive Review in Food Science and Food Safety, 16(1), pp. 1243-1268.
Pravekar, P., Palaskar, J., Metgud, S., Maria, R. & Dutta, S., 2020. The Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC) of Silver Nanoparticles Against Staphylococcus aureus. Biomaterial Investigations in Dentistry, 7(1), pp. 105-109.
Pratiwi, R. H., Hanafi, M., Artanti, N. & Pratiwi, R. D., 2018. Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Nessia altissima. The Journal of Tropical Life Science, 8(1), pp. 37-42.
Rao, K. V. R., Mani, P., Satyanarayana, B. & Rao, T. R., 2017. Purification and Structural Elucidation of Three Bioactive Compound Isolated from Streptomyces coelicoflavus BC 01 and Their Biological Activity. Biotech , 7(24), pp. 1-12.
Racmawati, F., Nuria, M. C. & Sumantri., 2011. Uji Aktivitas Antibakteri Fraksi Kloroform Ekstrak Etanol Pegagan (Centella asiatica(L) Urb) Serta Identifikasi Senyawa Aktifnya. Prosiding Seminar Nasional Peranan dan Kontribusi Herbal dalam Terapi Penyakit Degeneratif, 1(1), pp. 7- 13.
Rahman, H., Kartawinata, T. G. & Julianti, E., 2012. Uji Aktivitas Enzim Superoksida Dismutase dalam Ekstrak Mesokarp Buah Merah (Pandanus conoideus Lamarck) Menggunakan Densitometri Citra Elektroforegram. Acta Pharmaceutica Indonesia, 38(2), pp. 43-47.
Rohadi, Raharjo, S., Falah, I. I. & Santoso, U., 2016. Aktivitas Antioksidan Ekstrak Biji Duwet (Syzygium cumini Linn.) pada Peroksidasi Lipida secara In Vitro. Agritech, 36(1), pp. 30-37.
Rozik, M., Sudianto, A. & Nuroyam, H., 2011. Aktivitas Enzim Superoksida Dismutase dan Enzim Protease pada Udang Windu (Penaeus monodon Fabricus) yang Diinfeksi Vibrio harveyi Pasca-Pemberian Imunostimulan OMP Vibrio alginolyticus. Berkas Penelitian Hayati Edisi Khusus,6, pp. 7-11.
Ryandini, D., Pramono, H. & Sukanto, 2018. Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria. Biosaintifika, 10(1), pp. 117-124.
Simanjuntak, P., Parwati, T., Lenni , L. E., Tamat, R. & Murwani, R, 2004. Isolasi dan Identifikasi Antioksidan dan Ekstrak Benalu Teh Isolasi Ortiana (Korth denses). Jurnal Ilmu Kefarmasian Indonesia , 5(1), pp. 19-24.
Singh, L. S., Sharma, H. & Talukdar, N. C., 2014. Production of Potent Antimicrobial Agent by Actinomycete, Streptomyces sannanensis Strain SU 118 Isolated from Phoomdi in Loktak Lake Manipur, India. BMC Microbiology, 14(278), pp. 1-14.
Sudirman, S., 2011. Aktivitas Antioksidan dan Komponen Bioaktif Kangkung Air (Ipomoea aquatic Forsk.). Skripsi. Bogor: IPB.
Sulistyo, 1971. Farmakologi dan Terapi. Yogyakarta: Penerbit EKG.
Syarifuddin, A., Sulistyani, N. & Kintoko., 2018. Aktivitas Antibiotik Isolat Bakteri Kp13 dan Analisa Kebocoran Sel Bakteri Escherichia coli. Jurnal Ilmu Kefarmasian Indonesia, 16(2), pp. 137-144.
Taecowisan, T., Chaisaeng, S. & Phutdhawong, W. S., 2017. Antibacterial, Antioxidant, and Anticancer Activities of Biphenyls from Streptomyces sp. BO-07: an Endophyte in Boesenbergia rotunda (L.) Mansf A. Food and Agricultural Immunology, 28(6), pp. 1330-1346.
Tan , L. T. H., Ser, H. L., Yin, W. F., Chan, K. G., Lee, L. H. & Goh, B. H., 2015. Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated From Malaysia Mangrove Soil. Frontier in Microbiology, 6(1316), pp. 1-12.
Tan, L. T. H., Chan, K. G., Chan, C. K., Khan, T. M., Lee, L. H. & Goh, B. H., 2018. Antioxidative Potential of a Streptomyces sp. MUM292 Isolated from Mangrove Soil. Hindawi BioMed Research International, 1(1), pp. 1-13.
Tan, L. T. H., Chan, K. G., Khan, T. M., Bukhari, S. I., Saokaew, S., Duangjai, A., Pusparajah, P., Lee, L. H. & Goh, B. H., 2017. Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties. Frontier in Pharmacology, 8(276), pp. 1-18.
Tortora, G. J., Funke, B. R. & Christine, L. C., 2002. Microbiology: An Introduction. San Fransisco: Pearson Education Inc.
Tyas, S. H., Syarifuddin, A. & Septianingrum, N. M., 2021. Optimasi Waktu Produksi Antibakteri Isolat Actinomycetes (Isolat TE 235) terhadap Aktivitas Antibakteri pada Escherichia coli dan Staphylococcus aureus. Jurnal Farmasi Sains dan Praktis, 7(1), pp. 15-24.
Wagner, H. & Bladt, S., 1996. Plant Drug Analysis: A thin Layer Chromatography Atlas. Berlin: Springer-Verlag.
Wall, P. E., 2007. Thin-Layer Chromatography: A Modern Practical Approach. United Kingdom: Royal Society of Chemistry.
Wardana, R. S., Ryandini, D. & Oedjijono, O., 2017. Antibacterial Capacity of Streptomyces Isolate from a Mangrove Plant Rhizosphere Avicennia marina. Scripta Biologica, 4(2), pp. 131-134.
Wulandari, L., 2011. Kromatografi Lapis Tipis. Jember: PT. Taman Kampus Presindo.
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Published
2023-02-12
How to Cite
KURNIAWATI, Pratiwi Kusuma; RYANDINI, Dini; HERNAYANTI, Hernayanti.
Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus.
BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 4, n. 3, p. 121-132, feb. 2023.
ISSN 2714-8564.
Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/4593>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.20884/1.bioe.2022.4.3.4593.
Section
Articles
