Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java
Detection Moleculer
Abstract
Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.
References
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Duncan, B. 1977. Urceolariid ciliates, including three new species, from cultured Philippine fishes. Trans. Amer. J Micros. Soc, 96 (1): 76-81.
Felsentein, J., 1978. Case in Wich Parsimony or Compatibility Methods Will Be Positively Misleading. Systematic Zoology, 27 (4): 401-410.
Fernando, C.H., Furtado, J.l., Gusse, A.V., Harek & kakoge, A. 1972. Method for the Study of Freshwater Fish Series. Canada: University of Waterloo.
Gargas, J., 1995. Internal Parasites of Fish: Cestode, Digneans, and Nematodes. J FAMA, 2 (1): 95.
Handayani, R., Adiputra, Y. T., & Wardiyanto, 2014. Identifikasi dan Keragaman Parasit pada Ikan Mas Koki (Carrasius auratus) dan Ikan Mas (Cyprinus carpio) yang Berasal dari Lampung dan Luar Lampung. Lampung: Jurnal Ilmu Perikanan dan Sumberdaya Perairan.
Handoyo, D., Ari R. 2000. Prinsip Umum dan Pelaksanaan Polymerase Chain Reaction (PCR) [General Principles and Implementation of Polymerase Chain Reaction]. Unitas, 9 (1): 17-29.
Irianto, A. 2005. Patologi Ikan Teleostei. Yogyakarta: Gadjah Mada University Press.
Kabata Z. 1985. Parasites and Diseases of fish Cultured In The Tropics. London: Taylor and Franscis.
Lom, J. 1962. Trichodinid ciliates from fishes of the Rumanian Black Sea Coast. Journal of Parasitology, 52 (1): 49-61.
Louie, M., Louie L., Simor A. E.. 2000. The Role of DNA Amplification Technology in the Diagnosis of Infectious Disease.Can Med Assoc J, 9 (1): 163-201.
Margolis, L. & Arthur, J. K., 1979. Synopsis of the Parasites of Fishes of Canada. Fish. Res. Board Can. Bull, 199 (1): 1-269.
McArdle, J. F. 1984. Trichodina as A Cause of Mortalities in Cange Reared Rainbow Trout (Salmo gairdneri) and Salmon (Salmo salar). Bulletin Europe Fish Pathology, 4(l): 3-6.
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Nugrayani, D., Agung, C.S., & Hamdan, S. 2011. Prevalensi Trichodina Pada Kondisi Lingkungan Perairan Berbeda. J Omni-Akuatika, 3 (13): 43 – 48.
Pramono, T.B & H. Syakuri. 2008. Infeksi Parasit Pada Permukaan Tubuh Ikan Nilem (Osteocillus hasellti) yang Diperdagangkan Di PPI Purbalingga. Berkala Ilmiah Perikanan, 3 (2): 1-15.
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Rokhmani, Edy Purwonohadi dan Siti Khasanah, 2015. Hubungan Kekerabatan protozoa Trichodina sp yang menginfeksi benih gurame dari sentra pembenihan di Jawa Tengah dan Pengendaliannya. Proseding Semnas - LPPM 5, Unsoed Purwokerto.
Rokhmani, Edy Riwidiharso, Endang Ariyani, Darsono dan Daniel D. W., 2016. Hubungan Kekerabatan protozoa Trichodina sp yang menginfeksi benih gurame dari sentra pembenihan di Pulau Jawa dan Pengendaliannya. Proseding Semnas LPPM 6. Unsoed Purwokerto.
Scholz, T., 1999. Parasite in Cultured and Feral Fish. Jerman: Veterinary Parasitology.
Suwanto, A. 2011. Keanekaragaman Hayati Mikroorganisme. Bogor: Jurusan Biologi FMIPA IPB.
Syafaruddin & Tri J. S., 2011.Optimasi Teknik Isolasi dan Purifikasi DNA yang Efisien dan Efektif pada Kemiri Sunan (Reutalis trisperma (Blanco) Airy Shaw).JLittri, 17(1) : 11-17.
Tang, F-H, Zao, Y-J, Waren A. 2013. Phyolgenic analysis of Thricodinids (Cilliophora, Oligohymenophora) inferred from 18S rRNA gene sequence data. Curr Microbiol, 66 (1): 306-311.
Tim Lentera., 2003. Cepat dan Tepat Memasarkan Gurami. Jakarta : AgroMedia Pustaka.
Van, A. J. G. & Basson, L. 1986. Trichodinid (Ciliophora: Peritrichida) Ectoparasites of Cultured Cichlids from Taiwan. Bulletin Institute. Zoology Academy Sinica, 25 (1): 135-139.
Van, A. J. G., L. Basson & J. Theron. 1984. An Experimental Evaluation of The Use of Formalin to Control Trichodiniasis and Other Ectoparasitic Protozoan on Fry of Cyprinus Carpio L. and Oreochromis Mossambicus (Peters). South African Journal of Wildlife Research, 13 (1): 42-48.
Windarto, R., Adiputra, Y.T., Wardiyanto & Efendi E., 2013. Keragaman Karakter Morfologi Antara Trichodina Nobilis dan Trichodina Reticulata pada Ikan Komet (Carrasius Auratus). e-Jurnal Rekayasa dan Teknologi Budidaya Perairan, 1(2) : 117-126.
Woo, J. L. 2006. Fish Disease and Disorder Parasite. University of Gu
Arthur, J. R. & J. Lom. 1984. Trichodinid Protozoa (Ciliophora: Peritrichida) from Fresh Water Fishes of Rybinsk Reservoir. USSR. Journal of Protozoology, 31 (1) : 82-91.
Basson, L. & Van As, J.G., 2006. Trichodinidae and Other Ciliophorans (Phyllum Ciliopora). In Woo, P.T.K (Ed), Fish Diseases and Disorders, Volume 1: Protozoan and Metazoan. Second Edition. London: CAB International Publishing, 5 (1): 154-182.
Basson, L., 2010. First records of Trichodinid ectoparasites (Ciliophora: Peritrichia) from included freshwaterfishes in Tasmania, Australia, with comments of Pathogenity. Acta Parasitologica, 49 (3): 253-265.
Duncan, B. 1977. Urceolariid ciliates, including three new species, from cultured Philippine fishes. Trans. Amer. J Micros. Soc, 96 (1): 76-81.
Felsentein, J., 1978. Case in Wich Parsimony or Compatibility Methods Will Be Positively Misleading. Systematic Zoology, 27 (4): 401-410.
Fernando, C.H., Furtado, J.l., Gusse, A.V., Harek & kakoge, A. 1972. Method for the Study of Freshwater Fish Series. Canada: University of Waterloo.
Gargas, J., 1995. Internal Parasites of Fish: Cestode, Digneans, and Nematodes. J FAMA, 2 (1): 95.
Handayani, R., Adiputra, Y. T., & Wardiyanto, 2014. Identifikasi dan Keragaman Parasit pada Ikan Mas Koki (Carrasius auratus) dan Ikan Mas (Cyprinus carpio) yang Berasal dari Lampung dan Luar Lampung. Lampung: Jurnal Ilmu Perikanan dan Sumberdaya Perairan.
Handoyo, D., Ari R. 2000. Prinsip Umum dan Pelaksanaan Polymerase Chain Reaction (PCR) [General Principles and Implementation of Polymerase Chain Reaction]. Unitas, 9 (1): 17-29.
Irianto, A. 2005. Patologi Ikan Teleostei. Yogyakarta: Gadjah Mada University Press.
Kabata Z. 1985. Parasites and Diseases of fish Cultured In The Tropics. London: Taylor and Franscis.
Lom, J. 1962. Trichodinid ciliates from fishes of the Rumanian Black Sea Coast. Journal of Parasitology, 52 (1): 49-61.
Louie, M., Louie L., Simor A. E.. 2000. The Role of DNA Amplification Technology in the Diagnosis of Infectious Disease.Can Med Assoc J, 9 (1): 163-201.
Margolis, L. & Arthur, J. K., 1979. Synopsis of the Parasites of Fishes of Canada. Fish. Res. Board Can. Bull, 199 (1): 1-269.
McArdle, J. F. 1984. Trichodina as A Cause of Mortalities in Cange Reared Rainbow Trout (Salmo gairdneri) and Salmon (Salmo salar). Bulletin Europe Fish Pathology, 4(l): 3-6.
Nuryanto Agus, H. Pramono, M. H. Sastranegara, 2017. Molecular Identification of Fish Larvae from East Plawangan of Segara Anakan Cilalcap, Central Java Indonesia . Jurnal Biosaintifika: Journal of Biology & Biology Education 9 : 33-40.
Nugrayani, D., Agung, C.S., & Hamdan, S. 2011. Prevalensi Trichodina Pada Kondisi Lingkungan Perairan Berbeda. J Omni-Akuatika, 3 (13): 43 – 48.
Pramono, T.B & H. Syakuri. 2008. Infeksi Parasit Pada Permukaan Tubuh Ikan Nilem (Osteocillus hasellti) yang Diperdagangkan Di PPI Purbalingga. Berkala Ilmiah Perikanan, 3 (2): 1-15.
Rokhmani. 2009. Keragaman dan Tingkat Serangan Ektoparasit pada Gurame (Osphronemus gouramy Lac.) Tahap Pendederan I dengan Ketinggian Lokasi Pemeliharaan yang Berbeda. Jurnal Biotika, 7 (2): 87-93.
Rokhmani, Edy Purwonohadi dan Siti Khasanah, 2015. Hubungan Kekerabatan protozoa Trichodina sp yang menginfeksi benih gurame dari sentra pembenihan di Jawa Tengah dan Pengendaliannya. Proseding Semnas - LPPM 5, Unsoed Purwokerto.
Rokhmani, Edy Riwidiharso, Endang Ariyani, Darsono dan Daniel D. W., 2016. Hubungan Kekerabatan protozoa Trichodina sp yang menginfeksi benih gurame dari sentra pembenihan di Pulau Jawa dan Pengendaliannya. Proseding Semnas LPPM 6. Unsoed Purwokerto.
Scholz, T., 1999. Parasite in Cultured and Feral Fish. Jerman: Veterinary Parasitology.
Suwanto, A. 2011. Keanekaragaman Hayati Mikroorganisme. Bogor: Jurusan Biologi FMIPA IPB.
Syafaruddin & Tri J. S., 2011.Optimasi Teknik Isolasi dan Purifikasi DNA yang Efisien dan Efektif pada Kemiri Sunan (Reutalis trisperma (Blanco) Airy Shaw).JLittri, 17(1) : 11-17.
Tang, F-H, Zao, Y-J, Waren A. 2013. Phyolgenic analysis of Thricodinids (Cilliophora, Oligohymenophora) inferred from 18S rRNA gene sequence data. Curr Microbiol, 66 (1): 306-311.
Tim Lentera., 2003. Cepat dan Tepat Memasarkan Gurami. Jakarta : AgroMedia Pustaka.
Van, A. J. G. & Basson, L. 1986. Trichodinid (Ciliophora: Peritrichida) Ectoparasites of Cultured Cichlids from Taiwan. Bulletin Institute. Zoology Academy Sinica, 25 (1): 135-139.
Van, A. J. G., L. Basson & J. Theron. 1984. An Experimental Evaluation of The Use of Formalin to Control Trichodiniasis and Other Ectoparasitic Protozoan on Fry of Cyprinus Carpio L. and Oreochromis Mossambicus (Peters). South African Journal of Wildlife Research, 13 (1): 42-48.
Windarto, R., Adiputra, Y.T., Wardiyanto & Efendi E., 2013. Keragaman Karakter Morfologi Antara Trichodina Nobilis dan Trichodina Reticulata pada Ikan Komet (Carrasius Auratus). e-Jurnal Rekayasa dan Teknologi Budidaya Perairan, 1(2) : 117-126.
Woo, J. L. 2006. Fish Disease and Disorder Parasite. University of Gu
Published
2021-04-10
How to Cite
ROKHMANI, Rokhmani; WAHYONO, Daniel Joko; MULYANI, Lilis.
Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java.
BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 3, n. 1, p. 26-32, apr. 2021.
ISSN 2714-8564.
Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/3924>. Date accessed: 23 apr. 2024.
doi: https://doi.org/10.20884/1.bioe.2020.2.3.3924.
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