Pengaruh Penambahan Prebiotik Inulin dan Fruktooligosakarida (FOS) terhadap Pertumbuhan Probiotik Bifidobacterium sp. Bb2E
Abstract
Human health is very closely related to the condition of the human digestive tract since the beginning of life. The diversity of microorganisms found in the human digestive tract is very diverse, consisting of 300-500 different species of bacteria to increase the working power of nutrition. Prebiotics such as inulin and FOS and probiotics such as Bifidobacteria are aspects that can be added for the increase of nutrition. Optimal bacterial growth can be seen in the bacterial growth curve. The research problems were how the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, the amount of incubation time needed to support the growth of Bifidobacterium sp. Bb2E, and how the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. The purposes of this study were to study the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, to know the optimal incubation time of Bifidobacterium sp. Bb2E, and to know the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. This research was conducted using a completely randomized design (CRD) with factorial patterns. The main parameter measured was the population of Bifidobacterium sp. Bb2E, and the additional parameters measured were the pH level and the value of lactic acid titrated. The independent variable discussed in this study is prebiotic estimation on the medium, while the dependent variable considered is the population of Bifidobacterium sp. BB2E. The results of this study showed that the addition of inulin and fructooligosaccharide prebiotic at different incubation times had a significant effect on the growth of Bifidobacterium sp. Bb2E. The best treatment was a combination of inulin + FOS at incubation time of 18 hours with an optical density value was 1,794 and a total population density was 2,44x1010 CFU/mL.
References
Collins, M. D., & Gibson G. R., 1999, Probiotics, Prebiotics, and Synbiotics: Approaches for Modulating the Microbial Ecology of the Gut. The American Journal of Clinical Nutritions, 69 : 1052S-1057S.
Fardiaz, S., 1990, Penuntun Praktek Mikrobiologi Pengolahan Pangan. Program Studi Ilmu Pangan. Program Pasca Sarjana. Bogor: Institut Pertanian Bogor.
Ganzle, M. G. & R. Follador, 2012, Metabolism of Oligosaccharides and Starch in Lactobacilli. Frontiers in Microbiology, 3(340): 1-15.
Hegar, B., 2017, Kesehatan Saluran Cerna pada Awal Kehidupan untuk Kesehatan pada Masa Mendatang. Jurnal Kedokteran Indonesia, 5(2): 73-77.
Indrato, A.F., Sulistyarsi, A. & Ardhi, M.W., 2017, Isolasi Bakteri Probiotik dari Usus Ikan Lele Untuk Fermentasi Yoghurt Sebagai Bahan Modul Berbasis Riset dan Keterampilan Proses Sains. In Prosiding Seminar Nasional SIMBIOSIS, 2 : 315-328.
Jagadeeshwari, P. A. T., 2019, Correlation of Direct Gram Smear Findings with Bacterial Isolation in Sputum of Patients. Indian Journal of Applied Research, 9(11) : 68-69.
Lopes, S.M.S., Francisco, M.G., Higashi, B., de Almeida, R.T.R., Krausová, G., Pilau, E.J., Gonçalves, J.E., Gonçalves, R.A.C. & de Oliveira, A.J.B., 2016, Chemical Characterization and Prebiotic Activity of Fructo-Oligosaccharides From Stevia Rebaudiana (Bertoni) Roots and In Vitro Adventitious Root Cultures. Carbohydrate polymers, 152 : 718-725.
Khoiriyah H, & Ardiningsih P., 2014, Penentuan Waktu Inkubasi Optimum terhadap Aktivitas Bakteriosin Lactobacillus Sp. RED. Jurnal Kimia Khatulistiwa, 3(4) : 14-20.
Markowiak, P. & Katarzyna S., 2017, Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients, 9(1021) : 1-30.
Martinez, F.A.C., Eduardo, M.B., Attilio, C., Paul D. C., & Ricardo, P.S.O., 2013. Bacteriocin Production By Bifidobacterium Spp. A Review. Biotechnology Advances, 31 : 482-488.
Nuraida, L., Nur R. M., Didah N. F., & Hana, 2011, Metabolisme Prebiotik Oleh Kandidat Probiotik Isolat ASI sebagai Dasar Pengembangan Produk Sinbiotik. Jurnal Teknol. dan Industri Pangan, 21(2) : 156-163.
Nurjannah, L., Suryani., Achmadi, S. S., & Azhari, A., 2017. Produksi Asam LaktatOleh Lactobacillus delbrueckii subsp. bulgaricus dengan Sumber Karbon Tetes Tebu. JTIP Indonesia, 9(1) 1-9.
Rossi, M., Corradini, C., Amaretti, A., Nicolini, M., Pompei, A., Zanoni, S. & Matteuzzi, D., 2005, Fermentation of Fructooligosaccharides and Inulin by Bifidobacteria: A Comparative Study of Pure and Fecal Cultures. Applied and environmental microbiology, 71(10) : 6150-6158.
Setiarto, R. H. B., Nunuk W., Iwan S., & Rina M. S., 2016, Pengaruh Variasi Konsentrasi Inulin pada Proses Fermentasi oleh Lactobacillus acidophilus, Lactobacillus bulgaricus dan Streptococcus thermophilus. Biopropal Industri, 8(1): 1-17.
Setiarto, R. H. B., Nunuk W., & Nimas A. R., 2017, Optimasi Konsentrasi Fruktooligosakarida untuk Meningkatkan Pertumbuhan Bakteri Asam Laktat Starter Yoghurt. Jurnal Veteriner, 18(3) : 428-440.
Sharah, A., Karnila, R., & Desmelati, 2015, Pembuatan Kurva Pertumbuhan Bakteri Asam Laktat yang di Isolasi dari Ikan Peda Kembung (Rastrelliger Sp.), JOM : 1-8.
Surono, I.S., 2004. Probiotik Susu Fermentasi dan Kesehatan. Jakarta:Tri Cipta Karya.
Usmiati, S. & Juniawati, 2011, Karakteristik Dadih Probiotik Menggunakan Kombinasi Lactobacillus casei, Lactobacillus plantarum, dan Bifidobacterium longum Selama Penyimpanan. Journal of Nutrition and Food, 6(1) : 1-12.
Vrese, D., & Marteau S., 2008, Probiotics, Prebiotics, and Synbiotics. Food Biotechnology, Advances in Biochemical Engineering: 1-66.
Wahyuningsih, N. & Zulaika, E., 2019, Perbandingan Pertumbuhan Bakteri Selulolitik pada Media Nutrient Broth dan Carboxy Methyl Cellulose. Jurnal Sains dan Seni ITS, 7(2): 36-38.
Yohan, Y., Astuti, F., & Wicaksana, A., 2018, Pembuatan Spektrofotometer Edukasi untuk Analisis Senyawa Pewarna Makanan. Chimica et Natura Acta, 6(3) : 111-115.
