Isolation and Characterization Of Arbuscular Mychorhiza Fungi from Gaharu Wood (Aquilaria spp.) Rhizosphere
Abstract
In the last 30 years there have been increasing demand of agarwood. Unlimited hunting of agarwood causing it become threatened species. Aquilaria spp. is the main genus producing agarwood that considered as threathened species. Conserving Aquilaria spp. as germ plasm needs is essential against destruction. Arbuscular mycorrhizal fungi (AMF) have been used as inoculant to increase the growth of Aquilaria spp., but information about the existence of AMF in Aquilaria spp. rhizosphere has not been reported. This research was carried out with objective to identify AMF from Aquilaria spp. rhizosphere. Soil and root samples from rhizosphere of Aquilaria spp. taken from four location that is: Carita (A. malaccensis), Ciapus (A. filaria), Cifor (A. crasna & A. malaccensis), and Bantar Kemang (A. beccariana, A. crasna, & A. malaccensis). Roots staining was conducted with modified Phillips and Hayman procedure (1970). Soils sample was run dry wed and placed in pot culture with Pueraria javanica as the host plant. Spore was separated from pot culture using wet sieving and decanting method continued with centrifugation. The result showed the occurrences of AMF in Aquilaria spp. roots i.e. external hypha, internal hypha, coiled hypha, arbuscules, and vesicles. All AMF colony structure found in Aquilaria spp. roots show asosiation bettwen the plant and AMF. Four species of AMF were found i.e. Glomus sp.1, Glomus sp.2, Gigaspora albida, and Dentiscutata sp.
Key Words: Isolation, Arbuscule Mycorrhizae Fungi, Rhizosfer, Agarwood
References
CITES. 2019. Cites appendices I,II and III. Tersedia pada: https://cites.org/ sites/default/files/eng/app/2019/E-Appendices-2019-11-26.pdf [Diakses 23 Mei 2020].
Gerdemann JW, Trappe JM. 1974. The Endogonaceae in the Pacififi c Northwest. Mycol Memoir, 5:1–76
Goss MJ, Cardavo M, Brito I. 2017. Functional Diversity of Mycorrhiza and Sustainable Agriculture. Academic Press. United Kingdom.
IUCN. 2018. Iucn redlist. Tersedia pada: https://www.iucnredlist.org/search: Aquilatia spp, [Diakses 23 Mei 2020].
INVAM. 2017a. Species Descriptions from Reference Cultures. Tersedia pada: http://fungi.invam.wvu.edu/the-fungi/species-descriptions.html [Diakses 02 Juni 2020].
INVAM. 2017b. Gigaspora albida (reference accession BR214). Tersedia pada: http://fungi.invam.wvu.edu/the-fungi/classification/gigasporaceae/gigaspora/albida.html [Diakses 05 Juni 2020].
INVAM. 2017c. Dentiscutata nigra (reference accession NC182). Tersedia pada: http://fungi.invam.wvu.edu/the-fungi/classification/gigasporaceae/dentiscutata/nigra.html [Diakses 22 Mei 2020].
INVAM. 2017d. Dentiscutata reticulata (reference accession CL202). Tersedia pada: http://fungi.invam.wvu.edu/the-fungi/classification/gigasporaceae/dentiscutata/reticulata.html [Diakses 09 Juni 2020].
Khade SW. 2010 – Dentiscutata nigerita - a new species of arbuscular mycorrhizal fungi from India. Mycosphere 1(3), 241–247.
Kurnia, Gusmiati, Larekeng SH. 2019. Identifikasi Dan Karakterisasi Mikoriza Pada Tegakan Nyatoh (Palaquium sp.). Jurnal Perennial, 15(1): 51-57.
Lee SY, Mohamed R. 2016. Chapter 1: The Origin and Domestication of Aquilaria, an Important Agarwood-Producing Genus. In: Mohamed, R. (editor) Agarwood: Science Behind the Fragrance. Singapore: Springer.
Muryati S, Mansur I, Budi SW. 2016. Keanekaragaman Fungi Mikoriza Arbuskula (Fma) Pada Rhizosfer Desmodium spp. Asal Pt. Cibaliung Sumberdaya, Banten. Jurnal Silvikultur Tropika 07(3):188-197.
Oehl F, Souza F, Sieverding E. 2008. Revision of Scutellospora and description of fi ve new genera and three new families in the arbuscular mycorrhiza forming Glomeromycetes. Mycotaxon 106:311–360
Phillps JM, Hayman DS. 1970. Improved procedures for staining roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55(1):158-161.
Redecker D, Schüßler A, Stockinger H, Stürmer SL, Morton JB. 2013. An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota). Mycorrhiza 23:515-531. doi: 10.1007/s00572-013-0486-y
Schüßler A, Schwarzott D, Walker C. 2001. A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol Res 105(12):1413-1421
Schüßler A, Walker C (2010) The Glomeromycota. A species list with new families and new genera. Arthur Schüßler & Christopher Walker, Gloucester. Tersedia pada: http://www.amf-hylogeny.com/Schuessler&Walker2010_Glomeromycota.pdf [Diakses 22 Mei 2020].
Souza T. 2015. Handbook of Arbuscular Mycorrhizal Fungi. Switzerland: Springer.
