The Effect Of Suplementation Chlorella vulgaris on Feed to Albumin Levels In Serum Nilem Fish (Osteochilus vittatus)
Abstract
Nilem fish belongs to freshwater fish commodity that has been widely cultivation. Some factors influencing fish cultivation are good quality of feed, quantity, size, and shape. Quality feed works as a primary energy source as well as improves the digestive system of fish, with the expectation that the fish growth and health are optimal. Information concerning health status in cultivation Nilem has been used as a reference for improving cultivation methods and controlling disease attacks. The population and production of fish cultivation is experiencing serious problems due to infection from various types of pathogens. Therefore, a viable alternative is needed to prevent and overcome diseases by utilizing immunostimulant substances derived from synthetic substances that can increase the activity of non-specific immune defenses and stimulate antibiotic-forming organs in fish body. Immunostimulant is a substance that is capable of increasing the mechanism of non-specific immune response of fish one of them is by adding Chlorella vulgaris extract to the feed. This research objective is to determine the influence of Chlorella vulgaris supplementation on total blood albumin in nilem fish. This research was conducted by sampling method consisting of 5 treatments with further 4 replications. The treatments were feed supplementation of 0 g.kg-1, 2 g.kg-1, 3 g.kg-1, 4 g.kg-1, and 6 g.kg-1. Independent and dependent variables were used in this research; the independent variable was the dose of Chlorella vulgaris in the feed and the dependent variable was blood albumin concentration. The measuring of plasma albumin concentration was done using the Dyasis kit method with a spectrophotometer. The obtained data were then analyzed by ANOVA, followed by the Least Significant Difference (LSD) at the 95% confidence level if there is a real difference then followed by Tukey's test at the same confidence level. The results showed that the average value of serum albumin concentration of nilem fish from all treatments was between 4.2761 – 7.1826 g/dL. The normal albumin concentration was found in the treatment 4 g.kg-1Chlorella vulgaris supplementation which was 4.77 g/dL. Meanwhile, the highest concentration was found from the supplementation of Chlorella vulgaris at 3 g.kg-1 feed which was 6.677 g/dL.
References
Adel, Milad, Sakineh Yeganeh, Maryam Dadar, Masahiro Sakai, Mahmoud AO Dawood. 2016. Effects of dietary Spirulina platensis on growth performance, humoral and mucosal imune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754). Fish and Shellfish Imunology. 2(56): 436-444
Agustin P.K.A., Sri H., Alfabetian H.C.H. 2017. Pengaruh Ekstrak Temulawak Pada Pakan Sebagai Imunostimulan Pada Ikan Tawes (Puntius Javanicus Dengan Uji Tantang Bakteri. Tembalang. Semarang
Akrami Raza, Ahmad Gharaei, Majid Razeghi Mansour, Ali Galeshi. 2015. Effects of dietary onion (Allium cepa) powder on growth, innate immune response and hematoebiochemical parameters of beluga (Huso huso Linnaeus, 1754) juvenile. Fish & Shellfish Immunology. 2(45); 828-834.
Becker, E.W., 2007. Microalgae as a source of protein. Biotechnol. Adv., 25(2), 207-210.
Cholik, F., Ateng GJ., R. P. Purnomo & Ahmad Z. 2005. Akuakultur Tumpuan Harapan Masa Depan Masyarakat Perikanan Nusantara dan Taman Akuarium Air Tawar. Jakarta: Viktoria Kreasi Mandiri.
Florensia S. P., Tri R.S., Munifatul I. 2015. Potensi Chlorella vulgaris Beijerinck Dalam Remidiasi Logam Berat Cd Dan Pb Skala Labolatorium. 16 (2). Tembalang. Semarang
Ginanjar, A. 2011. Pengaruh Bobot Ikan Nilem (Osteochilus hasselti) Terhadap Tingkat Konsumsi Perifiton. Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran, Bandung.
Guardiola FA, Bahi A, Esteban MA. 2018. Effects of dietary administration of fenugreek seeds on metabolic parameters and immune status of gilthead seabream (Sparus aurata L.), Fish and Shellfish Immunology. (23) : 34-40.
Hiroko O., Takeo N., Yuki K., Yuki. 2017. Serum albumin levels and their correlates among individuals with motor disorders at five institutions in Japan. Kyoto University School of Public Health. Japan
Hoseini Seyyed Morteza, Morteza Yousefi, Seyed Hossein Hoseinifar, Hein Van Doan. 2018. Effects of dietary arginine supplementation on growth, biochemical, and immunological responses of common carp, (Cyprinus carpio L.), stressed by stocking density. Journal Aquaculture.
Kaslow JE. 2010. Analysis of Serum Protein. Santa Ana: 720 North Tustin Avenue Suite 104, CA
Kumar S, Raman RP, Prasad KP, Srivastva PP, Kumar S, Rajendran KV. 2017. Modulation of innate immune responses and induction of oxidative stress biomarkers in Pangasianodon hypophthalamus following an experimental infection with dactylogyrid monogeneans. Fish and Shellfish Immunology. (34): 384-890.
Liu L., Georg P., Dong W. 2016. Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential. China University of Technology. China
Matheus N. 2013. The Isolation of Albumin and Characteristics of Molecular Weight from Extraction Result by Steaming of Fish Gabus (Ophiocephalus striatus). Fakultas Pertanian Universitas Yudharta Pasuruan. Jawa Timur
Merlyn D., Manurung., Reiny A., Tumbo., Henneke P., Deiske A. S., Remy E. P., Mangindaan. 2019. Pemanfaatan Ekstrak Kasar Spons Untuk Meningkatkan Pertumbuhan Dan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus). ISSN 2302-3589. Universitas Sam Ratulangi. Manado
Pratiwi., Rita R., Yayat D. 2011. Pengaruh Tingkat Pemberian Pakan Terhadap Laju Pertumbuhan Dan Deposisi Logam Berat Pada Ikan Nilem Di Karamba Jaring Apung Waduk IR. H. Djuanda. ISSN 0853-2523. Fakultas Perikanan dan Ilmu Kelautan Kampus Jatinangor. Bandung
Řehulka, J. (1993). Erythrodermatitis in Carp (Cyprinus carpio L.): An Electroforetic Study of Blood Serum Protein Fraction Levels. Acta Veterinaria Brno, 62(3-4): 187-197.
Royan, M. R., Khomaruddin., M. D. ArifidanMinto. 2010. Chlo-Juice (Jus Chlorella) Sebagai Minuman Multivitamin Berkhasiat, Berkalsium, Dan BerproteinTingi Serta Sebagai Peluang Usaha Multiprofit. PKMK. UniversitasAirlangga. Surabaya. 16 pp
Salasia, Oktavia, S.I., Hariono, B. 2010. Patologi Klinik. Samudra Biru. Yogyakarta.
Simanjuntak, S.B.I., Edy, Y., Farida, N, H. 2006.Pengaruh Penyuplemenan Spirulina Dalam Pakan Terhadap Hematologis Ikan Nilem (Osteochilus hasselti C.V). Universitas Jenderal Soedirman. Purwokerto. Indonesian
Simanjuntak, S.B.I., Soedibya, P.H.T., & Wibowo, E.S. 2014. Growth performance of gurami (Osphronemus gouramy Lac.) the given phytoplankton Spirulina platensis and Chlorella vulgaris: 157-165.
Silva, Dilson., Celia Martins Cortez., Natalia P. Nogueira., dan Frederico Freire da Cunha Bastos. 2015. Purification and physico-chemical study of serum albumins of two neotropical fish species from the Sa˜o Francisco River Bassin, Brazil. Biota Neotropica. : 15 (3).
Siringi, J.O., Kang ombe, J., Jere WW., Mtethiwa A.H. 2016. Effect of dietary spirulina on growth, haematological factors and survival of Oreochromis shiranus challenged with Aeromonas hydrophila. Ruforum. 3(14)
Sus D. W., Anita E., Herlina. 2011. Profil Protein Total Albumin Dan Globulin Pada Ayam Broiler Yang Di beri Kunyit, Bawang Putih Dan Zink (Zn). ISSN 0853-4217 Vol. 16 No.3. Patologi Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Bogor.
Siti Y, R., Anhar S., Suradi W. S. 2014. Aspek Pertumbuhan Dan Reproduksi Ikan Nilem (Osteochilus haselti) Di Perairan Rawa Pening Kecamatan Tuntang Kabupaten Semarang. Universitas Diponegoro Tembalang, Semarang, Jawa Tengah
Yageneh, Sakineh. Mahdi Teomori. 2015. Dietary effects of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Research in Veterinary Science.
Yang X, Guo J, Ye J, Zhang Y, Wang W. 2014. The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp, Ctenopharyngodon idella, Fish and Shellfish Immunology.
