Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Ipomoea sp. Rhizospheres Growing in Iron Sand Soil

  • niharoh nurainy student
  • Oedjijono Oedjijono Universitas Jenderal Soedirman
  • Ardhini Rin Maharning Universitas Jenderal Soedirman

Abstract

Iron  sand  field,  is  mostly  found  along  the  Indonesia  coast. It has  low  organic  matter, contains 38-59% iron (Fe) and sand particles. These characteristics can be called as extreme environments, however there are bacteria capable of growing and surviving in such habitats. Several genera are known as PGPR agents  such as Rhizobium, Azospirillum, Azotobacter and Pseudomonas. The research objectives were to measure total population of bacteria from rhizosphere of Ipomoea sp. in iron sand soils, to investigate the ability of bacterial isolates capable of fixing nitrogen, solubilizing phosphate, and producing plant growth hormone such as IAA, and to identify plant growth promoting rhizobacteria isolated from plant rhizospheres candidates growing in iron sand soils. Isolation on NA medium showed that the population were ranged from 1.59 x 105 to 5.2 x 105 CFU.g-1. There were 22 bacterial isolates originated  from the media of Ashby, Caceres, and Pikovskaya. Six isolates (A4, A10, C10, P2, P3, and P4) showed high ability to fix nitrogen, solubilize phosphate, and produce IAA. Isolate P4 grew in nitrogen fixing and phosphate solubilizing assay as well as IAA producing. It showed high value of phosphate index (275 mm). Bacterial identification indicated that four isolates (C10, P2, P3, P4) were species members of genus Bacillus and two isolates (A4, A10) were identified as species members of Actinomycetes.

References

Amri, M. F., 2018. The Resistance of Rhizobacteria Isolated from Rhizosphere of Ipomoea sp. Growing in Iron Sand Soils towards Chromium. Skripsi. Purwokerto: Fakultas Biologi.

Backman, P.A., Brannnen, P.M., & Mahaffe, W.F., 1994. Plant Respon and Disease Control Followin Seed Inoculation with Bacillus sp. Australia: Pruc Third Int Work PGPR South Australia.

Budiyanto, M. A. K. 2004. Mikrobiologi Terapan. Malang: Universitas Muhammadiyah Press.

De Vos, P., Garrity, G. M., Staley J. T., Krieg, N. R., Brenner, D. J., Rainey, F. A., Schleifer K. H. &. Boone, D. R., 2009. Bergey’s Manual of Systematic Bacteriology Third Edition Volume Three The Firmicutes II. 3rd ed. New York: Springer.

Erfin, Sandiah, N., & La Malesi., 2016. Identifikasi Bakteri Azospirillum dan Azotobacter pada Rhizosphere asal Komba-Komba (Chromolaena odorata). JITRO, 3(2):31-38.

Ferfinia, A., 2010. Eksplorasi bakteri dan cendawan rizofer yang berasosiasi dengan penyakit busuk basah pada batang pepaya (Carica papaya) di Pasir Kuda, Desa Ciomas, Bogor. Skripsi. Departemen Pertanian Fakultas Pertanian IPB.

Gordon, S.A. & Weber, R.P. 1951. Colorimetric Estimation of Indole Acetic Acid. Plant Physiol. 26: 192-195.

Gravel, V., Antoun, H., & Tweddel, R.J. 2007. Effect of Indole Acetic Acid (IAA) on the Development of Symptoms Caused by Pytium ultimum on Tomato Plants. Eur. J. Plant. Pathol. 119: 457-462.

Herlina, L., Pukan, K. K., & Mustikaningtyas, D., 2016. Kajian Bakteri Endofit Penghasil IAA (Indole Acetic Acid) untuk Pertumbuhan Tanaman. Sainteknol, 14(1): 51-58.

Hussain, M.I., Ashgar, H.N. & M. Arshad, a.M.S., 2013. Screening of Multi-traits Rhizobacteria to Improve Maize Growth Under Axenic Conditions. The Journal of Animal & Plant Sciences, 23(2):514-20.

Kafrawi, Kumalawati, Z. & Muliani, S., 2015. Skrining Isolat Plant Growth Promoting Rhizobacteri (PGPR) dari Pertanaman Bawang Merah (Allium ascalonicum) di Gorontalo. In Prosiding Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan. Makassai, 2015. Jurusan Biologi, Fakultas Sains dan Teknologi, UIN Alauddin Makassar.

Kanimozhi, K. and Panneerselvam, A. 2010. Studies on Isolation and Nitrogen Fixation Ability of Azospirillum spp. Isolated from Thanjavur District. Der Chemica Sinica, 1(3):138-145.

Kholida, F.T., & Zulaika, E., 2015. Potensi Azotobacter sebagai Penghasil Hormon IAA (Indole-3-Acetic Acid). Jurnal Seni dan Sains ITS, 4(2):2337-3520.

Kuster, E., & Williams S., 1964. Selection of Media for Isolation of Streptomyces. Nature, 2 (2):928-929.

Oedjijono., Soetarto, E.S., Moeljopawiro, S. & Djatmiko, H.A., 2014. Promising Plant Growth Promoting Rhizobacteria of Azospirillum spp. Isolated from Iron Sand Soils, Purworejo Coast, Central Java, Indonesia. Advances in Applied Science Research, 5(3):302-08.

Plante, A. F. 2007. Soil Biogeochemical Cycling of Inorganic Nutrients And Metals. Soil Microbiology, Ecology, and Biochemistry. Third edition. USA: Elsevier Inc.

Rao, S., 1994. Mikrobiologi Tanah. Malang: UMM Press.

Stoate C, N.D Boatman, R.J Borralho, C.R Carvalho, G.R de Snoo, and P. Eden. 2001. Ecological impacts of arable intensification in Europe. J Environ Manage, 63(4): 337-65.

Sutariati, G A.K., Rakian, T.C., Agustina., Sopacua, N., La Mudi & Haq, M., 2014. Potential Study of Plant Growth Promoting Rhizobacteria Isolated from Healthy Rice Rhizosphere. Jurnal Agroteknos, 4(2):71-77.

Vacheron, J., Desbrosses, G., Bouffaud, M. L., Touraine, B., Moënne-Loccoz, Y., Muller, D., Legendre, L., Wisniewski-Dyé, F., & Prigent-Combaret, C. 2013. Plant growth-promoting rhizobacteria and root system functioning. Frontier Plant Science, 4(356):1-19.

Widawati, S., Suliasih, H.J.D. Latupapua, Sugiharto, A., 2005. Biodiversity of Soil Microbes from Rhizosphere at Wamena Biological Garden (WBiG), Jayawijaya, Papua. Biodiversitas, 6(1): 6-11.

Widawati, S & Suliasih, 2006, Augmentasi bakteri pelarut fosfat (BPF) potensial sebagai pemacu pertumbuhan caysin (Brasica caventis oed) di tanah marginal, Jurnal Biodiversitas, 7(1):10-14.

Widawati, S. & Suliasih, 2006, Populasi Bakteri Pelarut Fosfat (BPF) di Cikaniki, Gunung Botol, dan Ciptarasa, serta Kemampuannya Melarutkan P Terikat di Media Pikovskaya Padat, Biodiversitas, 7 (2):109-113.

Widawati, S., 2015. Isolation And Activity of Plant Growth Promoting Rhizobacteria (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) from Soil of Rubber Plantation, Lampung. Berita Biologi, 14(1):77-88.

Wuryanto, S., 2015. Karakterisasi Isolat Azospirillum spp. Asal Lahan Pasir Besi sebagai Kandidat PGPR dan Kemampuannya Mendukung Pertumbuhan Vegetatif Tanaman Jagung (Zea mays L.). Skripsi. Purwokerto: Fakultas Biologi.

Youssef, M.M.A., & M.F.M Eissa. 2014. Biofertilizers and their role in management of plant parasitic nematodes. A review. E3 J Biotechnol Pharm Res. 13:1–6.

Yusron., & Azwar, Moh., 2012. Pengaruh Beberapa Jenis Pupuk Hayati terhadap Pertumbuhan Semai Sengon (Paraserianthes falcataria (L) Nielsen) pada Medium Tumbuh Tanah Bekas Tambang Emas. Skripsi. Universitas Tadulako Palu. Sulawesi Tengah.

Zainudin, Abadi, A.L. & Aini, L.Q., 2014. The Effect of Plant Growth Promoting Rhizobacteria Bacillus subtilis and Pseudomonas fluorescense to Downy Mildew of the Maize (Zea mays L.). Jurnal HPT, 2(1): 11-18.

Zulaika. 2015. Isolat Bacillus pelarut fosfat dari Kalimas Surabaya. Surabaya: ITS.
Published
2020-04-29
How to Cite
NURAINY, niharoh; OEDJIJONO, Oedjijono; MAHARNING, Ardhini Rin. Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Ipomoea sp. Rhizospheres Growing in Iron Sand Soil. BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 2, n. 1, p. 138-145, apr. 2020. ISSN 2714-8564. Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/1845>. Date accessed: 20 apr. 2021. doi: https://doi.org/10.20884/1.bioe.2020.2.1.1845.
Section
Articles