Karakterisasi Isolat Khamir Gf1 Terhadap Kondisi Asam Saluran Pencernaan Unggas

  • Awwaluz Zahroh Mahya Ainillah Universitas Jenderal Soedirman
  • Nuraeni Ekowati
  • Ade Erma Suryani

Abstract

Probiotik merupakan mikroorganisme yang dapat memperbaiki mikroekologi usus yang berdampak positif terhadap kesehatan inang. Kandidat probiotik merupakan  mikroorganisme yang memiliki potensi sebagai probiotik, seperti menghasilkan enzim fitase sehingga mampu mengoptimalkan kondisi saluran pencernaan.  Salah satu mikroorganisme sebagai kandidat probiotik adalah khamir penghasil enzim fitase. Khamir penghasil fitase telah diisolasi oleh BPTBA-LIPI Yogyakarta asal makanan  fermentasi tradisional Indonesia yaitu gathot.Pengujian khamir fitase sebagai kandidat probiotik dilakukan dengan menstimulasikan kondisi saluran pencernaan seperti kondisi asam yaitu pada  pH rendah dan  kondisi asam lambung. Kondisi pH yang rendah dan asam lambungmerupakan suatu penghalang masuknya mikroorganisme ke saluran gastrointestinal, oleh karena itu pengujian terhadap asam dilakukan untuk mengetahui karakteristik mikroorganisme yang akan digunakan sebagai kandidat probiotik. Pengujian dilakukan secara invitro dengan menguji ketahanan khamir pada kondisi asam dengan  nilai pH 2 dan 3 serta kondisi asam lambung dengan menggunakan pepsin 0,3%. Hasil penelitian menunjukkan bahwa isolat khamir GF1 asal makanan tradisional fermentasi mampu tahan terhadap kondisi asam karena mampu mempertahankan viabilitasnya pada pH 3 dan kondisi asam lambung.

Author Biographies

Nuraeni Ekowati

Fakultas Biologi Unsoed

Ade Erma Suryani

BPTBA-LIPI Yogyakarta

References

Brandão, R. L., Rosa, J. C. C., Nicoli, J. R., Almeida, M. V. S., do Carmo, A. P., Queiros, H. T., & Castro, I. M., 2014. Investigating acid stress response in different Saccharomyces strains. Journal of Mycology: 1-9

Chen, L. S., Ma, Y., Maubois, J. L., He, S. H., Chen, L. J., Li, H. M., 2010. Screening for the potential probiotic yeast strains from raw milk to assimilate cholesterol. Dairy Science and Technology, 90: 537-548.

Damayanti, E., Julendra, H., Sofyan, A., & Hayati, S. N., 2014. Bile salt and acid tolerant of lactic acid bacteria isolated from proventriculus of broiler chicken. Media Peternakan, 37(2): 80-86.

Duke, G. E., 1986. Alimentary canal: Anatomy, regulation of feeding, and motility. Pages 269–288 in Avian Physiology. P. D. New York: Sturkie, ed. Springer-Verlag.

Fadda, M. E., Mossa, V., Deplano, M., Pisano, M. B., & Cosentino, S., 2017. In vitro screening of Kluyveromyces strains isolated from Fiore Sardo cheese for potential use as probiotics. LWT-Food Science and Technology, 75: 100-106.

Fietto, J. L. R., Araujo, R. S., Valadao, F.N., Fietto, L.G., Brandao, R.L., Neves, M. J., Gomes, F. C. O., Nicoli, J. R. and Castro, I. M. 2004. Molecular and physiological comparison between Saccharomyces cerevisiae and Saccharomyces boulardii. Candian Journal of Microbiology, 50: 615-621.

Hu, X. Q., Liu, Q., Hu, J. P., Zhou, J. J., Zhang, X., Peng, S. Y., ... & Wang, X. D. 2018. Identification and characterization of probiotic yeast isolated from digestive tract of ducks. Poultry science, 97(8): 2902-2908.

Illeghems, K., De Vuyst, L. & Weckx, S., 2013. Complete genome sequence and comparative analysis of Acetobacter pasteurianus 386B, a strain well-adapted to the cocoa bean fermentation ecosystem. BMC Genomics, 14: 526.

Khidhr, K. O., & Zubaidy, Z. M. A., 2014. Isolation and Identification of Saccharomyces cerevisiae var boulardii and its Uses as a Probiotic (in vitro). Rafidain journal of science, 25(1E): 1-11.

Kumura, H., Tanoue, Y., Tsukahara, M., Tanaka, T., & Shimazaki, K. 2004.Screening of dairy yeast strains for probiotic applications. Journal of dairy science, 87(12): 4050-4056.

Lara-Hidalgo, C., Hernández-Sánchez, H., Hernández-Rodríguez, C., & Dorantes-Álvarez, L., 2017. Yeasts in Fermented Foods and their Probiotic Potential. Austin Journal of Nutrition & Metabolism, 4(1045): 1-8.

Moradi, R., Nosrati, R., Zare, H., Tahmasebi, T., Saderi, H., & Owlia, P., 2018. Screening and characterization of in-vitro probiotic criteria of Saccharomyces and Kluyveromyces strains. Iranian Journal of Microbiology, 10(2): 121-131.

Matjik, A. A. ,& Sumertajaya, I. M. 2006. Perancangan Percobaan dengan Aplikasi SAS dan Minitab. Ed ke-2. Jakarta (ID): LIPI Pr.

Niu, C., Luo, H., Shi, P., Huang, H., Wang, Y., Yang, P., & Yao, B., 2016. N-Glycosylation Improves the Pepsin Resistance of Histidine Acid Phosphatase Phytases by Enhancing Their Stability at Acidic pHs and Reducing Pepsin's Accessibility to Its Cleavage Sites. Applied and Environmental Microbiology, 82(4): 1004-1014.

Ogunremi, O. R., Sanni, A. I., & Agrawal, R., 2015. Probiotic potentials of khamirs isolated from some cereal based Nigerian traditional fermented food products. Journal of applied microbiology, 119(3): 797-808.

Oktaviana, R. S., 2014. Pengaruh Substitusi Puree Gatot Instan Terhadap Sifat Organoleptik Roti Manis. Jurnal Tata Boga, 3(3): 141-150

Ouwehand, A. C., Kirjavainen, P. V., Shortt, C., &Salminen, S., 1999. Probiotics: mechanisms and established effects. International Dairy Journal, 9: 43-52.

Psomas, E.I., Andrighetto, C., Litopoulou- Tzanetaki, E., Lombardi. A. and Tzanetakis, N., 2001. Some probiotic properties of yeasts isolates from infant faeces and feta chees. International Journal Food Microbiology, 69: 125-133.

Rajkowska, K., & Kunicka-Styczynska, A. 2010. Probiotic properties of yeasts isolated from chicken feces and kefirs. Pol J Microbiol, 59(4): 257-263.

Smith, J. L., 2003. The role of gastric acid in preventing foodborne disease and how bacteria overcome acid conditions. Journal of food protection, 66(7): 1292-1303.

Sorrells, K. M., & Leonard, B., 1988. Mechanism of acid tolerance by a yeast isolated from spoiled ketchup. Journal of Food Protection, 51(6): 489-490.

Syal, P & Vohra, A., 2013. Probiotic Potential of Yeasts Isolated From Traditional Indian Fermented Foods. International Journal of Microbiology Research, 5: 390-398.
Published
2020-04-29
How to Cite
AINILLAH, Awwaluz Zahroh Mahya; EKOWATI, Nuraeni; SURYANI, Ade Erma. Karakterisasi Isolat Khamir Gf1 Terhadap Kondisi Asam Saluran Pencernaan Unggas. BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 2, n. 1, p. 29-33, apr. 2020. ISSN 2714-8564. Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/1745>. Date accessed: 09 feb. 2023. doi: https://doi.org/10.20884/1.bioe.2020.2.1.1745.
Section
Articles