Pengaruh Antioksidan Alami pada Berat Testis Mencit (Mus musculus) yang Terpapar Senyawa Kimia Plastik

  • Atang Atang Fakultas Biologi Universitas Jenderal Soedirman
  • Endah Sri Palupi Fakultas Biologi Universitas Jenderal Soedirman


Plastic is a material that is often found in everyday life, both for household needs, offices, agriculture/plantations, industry, etc. One type of plastic that is commonly used is polycarbonate plastic (polycarbonate / PC). The main ingredient in polycarbonate plastics is a compound 2,2-bis (4-hydroxyphenyl) propane known as bisphenol A (BPA). The purpose of this study was to determine the effect of natural antioxidants on the testicular weight of adult male mice exposed to the chemical compounds that make up the plastic. This study used a completely randomized design (CRD) with six treatments and five replications. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. Plastic boiled water and antioxidants were given orally to 0.25 ml of male mice every day during the study. Quantitative data were analyzed using ANOVA and BNT. Based on the results of the research that has been carried out, it can be concluded that the provision of natural antioxidants for 21 days in mice (Mus musculus) exposed to plastic chemical compounds has an effect on decreasing the testicular weight of male mice in K (-) treatment / negative control. Based on data analysis through statistical tests that have been carried out, the series of natural antioxidant treatments for mice exposed to plastic chemical compounds for 21 days did not significantly affect the testicular weight of mice.


Andriawati, F. (2009). Efek Amfetamin Terhadap Spermatogenesis Mencit.
Birnbaum, L. S., Bucher, J. R., Collman, G. W., Zeldin, D. C., Johnson, A. F., Schug, T. T., & Heindel, J. J. (2012). Consortium-based science: the NIEHS’s multipronged, collaborative approach to assessing the health effects of bisphenol A. Environmental health perspectives, 120(12), 1640-1644.
Geneser, F. (1994). Text Book of Histology (Buku Teks Histologi) Jilid 2 Terjemahan F. Arifin Gunawijaya. Jakarta: Binarupa Aksara.
Gurmeet, K.S.S, Rosnah, I, Normadiah, M.K, Das, S, Mustafa, A.M. (2014). Detrimental effects of bisphenol A on development and functions of the male reproductive system in experimental rats. Excli journal. 13:151-160.
Halliwell, B., & Whiteman, M. (2004). Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. British journal of pharmacology, 142(2), 231-255.
Halden, R. U. (2010). Plastics and health risks. Annual review of public health, 31, 179-194.
Hariyatmi. (2004). Kemampuan vitamin e sebagai antioksidan Terhadap radikal bebas pada lanjut usia. Jurnal MIPA vol 14 No.1.Surakarta. UMS
Jin P, Wang X, Chang F, Bai Y, Li Y, Zhou R,. 2013. Low dose bisphenol A impairs spermatogenesis by suppressing reproductive hormone production and promoting germ cell apoptosis in adult rats. Journal of Biomedical Research, 27(2), 135–44. JBR.27.20120076.
Khaira, K., (2010). Menangkal Radikal Bebas dengan Anti-oksidan. Sainstek: Jurnal Sains dan Teknologi, 2(2), pp.183-187.
Liu C, Duan W, Li R, Xu S, Zhang L, Chen C,. (2013). Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity. Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity. Cell Death and Disease, 4(6). 10.1038/cddis.2013.203.
Nakamura D, Yanagiba Y, Duan Z, Ito Y, Okamura A, Asaeda N,. (2010). Bisphenol A may cause testosterone reduction by adversely affecting both testis and pituitary systems similar to estradiol. Toxicology Letters, 194(1–2), 16–25.
National Toxicology Program (NTP). (2008). NTP-CERHR monograph on the potential human reproductive and developmental effects of bisphenol A. Ntp Cerhr Mon., 22(08), 1–321.
Nurliani, A. (2004). Gambaran Struktur Mikroanatomi Tubulus Seminiferus Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Kulit Batang Durian (Durio zibethinus Murr). Unpublished Bachelor thesis, FMIPA UNLAM. Banjarbaru.
PPLH. (2007). Pendidikan Lingkungan Serial Global Warming. Seloliman: Divisi Penerbitan dan Dokumentasi Pusat Pendidikan Latihan Hidup (PPLH).
Rahman, M.S, Kwon, W.S, Lee, J.S, Yoon, S.J, Ryu, B.Y, Pang, M.G. (2015). Bisphenol-A affects male fertility via fertility-related proteins in spermatozoa. Scientific Reports. 5:9169. Doi: 10.1038/srep09169.
Rahmi, H., (2017). Aktivitas Antioksidan dari Berbagai Sumber Buah-buahan di Indonesia. Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech), 2(1).pp. 34-38.
Tremallen, K. (2008). Oxidative Stress and Male Infertility a Clinical Perspective. Human Reprod.Vol 14: 243- 258.
Yuyun A, Gunarsa D. (2011). Cerdas Mengemas Produk Makann dan Minuman. Jakarta: PT Agromedia Pustaka.
Xi, W, Wan, H.T, Zhao, Y.G, Wong, M.H, Giesy, J.P, Wong, C.K.C. (2012). Effects of perinatal exposure to bisphenol A and di(2-ethylhexyl)-phthalate on gonadal development of male mice. Environ Sci Pollut Res. 19:2515–2527.
Widiastuti, E.L., Arifianti, R., Khairani, I.A., Christianto, Y., Ara, N.F. & Maharani, H.W., (2019). Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo (α) pyrene. In IOP Conference Series: Earth and Environmental Science, 305(1), pp. 1-9.
How to Cite
ATANG, Atang; PALUPI, Endah Sri. Pengaruh Antioksidan Alami pada Berat Testis Mencit (Mus musculus) yang Terpapar Senyawa Kimia Plastik. Jurnal READ (Research of Empowerment and Development), [S.l.], v. 1, n. 2, p. 43-48, dec. 2020. ISSN 2745-4746. Available at: <>. Date accessed: 25 feb. 2021. doi:

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.