UJI AKTIVITAS ANTIBAKTERI DAN FITOKIMIA KULIT BAWANG MERAH (Allium cepa L.) HASIL EKSTRAKSI METODE Microwave Assisted Extraction (MAE)

Microwave-Assisted Extraction Method (MAE) On Antibacterial Activity and Phytochemical Screening of Onion skin (Allium Cepa L.)

  • Fitria Dewi Sulistiyono Program Studi Farmasi, Fakultas MIPA Universitas Pakuan, Bogor
  • Trirakhma Sofihidayati Program Studi Farmasi, Fakultas MIPA Universitas Pakuan, Bogor
  • Bina Lohitasari Program Studi Farmasi, Fakultas MIPA Universitas Pakuan, Bogor

Abstract

Ekstrak kulit bawang merah mengandung flavonoid, polifenol, saponin, terpenoid dan alkaloid. Metode ekstraksi modern dengan memanfaatkan radiasi gelombang mikro yang disebut dengan MAE (Microwave Assisted Extraction) belum banyak dilakukan. Metode MAE terbukti lebih efektif karena pemanasan pelarut secara cepat dan efisien dibandingkan metode ekstraksi secara konvensional. Tujuan penelitan ini adalah mengetahui senyawa fitokimia dan aktivitas ekstrak kulit bawang merah dengan metode MAE pada Staphylococcus aureus. Deteksi senyawa fitokimia dilakukan dengan metode kualitatif, yaitu ada tidaknya senyawa flavonoid, saponin, alkaloid dan tanin. Aktivitas ekstrak kulit bawang merah terhadap S.aureus dilakukan dengan metode kertas cakram dengan melihat zona bening/hambat yang dihasilkan. Konsentrasi ekstrak yang digunakan adalah 5, 10, 15, 20 dan 25% (b/v). Hasil penelitian menunjukkan bahwa ektrak kulit bawang merah mempunyai senyawa flavonoid, saponin dan tanin. Ekstrak kulit bawang merah dapat menghambat pertumbuhan S.aureus ditunjukkan dengan adanya zona bening dari kosentrasi 5, 10, 15, 20 dan 25% (b/v) adalah 14; 15,5; 16; 19; 19,5 mm. Hasil tersebut menunjukan bahwa MAE lebih efektif daripada metode konvensional (maserasi) dalam menghambat S. aureus. Berdasarkan hasil aktivitas antibakteri, ekstrak kulit bawang merah dapat dimanfaatkan dalam dunia industri sebagai salah satu komponen produk pencegahan infeksi bakteri.


 


Onion skin extract containing flavonoid , catakin , saponin , terpenoid and alkaloid . Microwave Assisted Extraction (MAE) is modern method with microwave radiation. MAE have proven more effective because a solvent warming quickly and more efficient compared other methode (maseration). This study aims to explore the phytochemical activity presenced in onion skin extract. that was obtained by the Microwave Assited Extraction (MAE) method and to evaluate the antibacterial activity against Staphilococcus aureus. The phytochemical screening of onion skin extracted by using MAE revealed the presence of alkaloids, saponins, tannins and flavonoids. The antibacterial activity of onion skin extract was carried out against S. aureus by measuring the diameter of bacterial growth inhibition zones through the diffusion method. The results of qualitative phytochemical screening tests on onion skin extract shows that the positive sample contains alkaloids, saponins, tannins and flavonoids. Range of onion skin extract concentration used in this research were 5, 10, 15, 20, and 25% w/v. The inhibition zone of extract at concentration 5, 10, 15, 20, and 25% w/v were 14.00; 15.50; 16.00; 19.00; and 19.50 mm respectively. Base on antibacterial result, onion skin extract can be used as one ingredient in the manufacture of antibacterial products.

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Published
2018-12-31
How to Cite
SULISTIYONO, Fitria Dewi; SOFIHIDAYATI, Trirakhma; LOHITASARI, Bina. UJI AKTIVITAS ANTIBAKTERI DAN FITOKIMIA KULIT BAWANG MERAH (Allium cepa L.) HASIL EKSTRAKSI METODE Microwave Assisted Extraction (MAE). Mandala Of Health : A Scientific Journal, [S.l.], v. 11, n. 2, p. 71-79, dec. 2018. ISSN 2615-6954. Available at: <http://jos.unsoed.ac.id/index.php/mandala/article/view/1316>. Date accessed: 28 sep. 2020. doi: https://doi.org/10.20884/1.mandala health.2018.11.2.1316.