Aktivitas Enzimatik Isolat Trametes spp. dari Kebun Raya Baturraden dalam Pewarna Batik dengan Variasi Konsentrasi Indigosol Blue Glukosa
Abstract
Indigosol Blue merupakan salah satu zat warna sintetik Antraquinon yang digunakan sebagai pewarna biru pada industri pencelupan tekstil dan bersifat rekalsitran dan non-biodegradable, sehingga tidak mudah rusak oleh perlakuan kimia maupun fotolitik. Isolat Trametes sp. diyakini memiliki kemampuan mentransformasi komponen pewarna melalui mekanisme degradasi enzimatik. Trametes sp. mampu menghasilkan enzim ekstraseluler ligninolitik yang dapat mendegradasi komponen xenobiotik dalam limbah pewarna indigosol menjadi bentuk yang tidak toksik di lingkungan. Penelitian bertujuan untuk mengetahui kemampuan tiga isolat uji dalam menghasilkan enzim dalam pewarna Indigosol Blue pada konsentrasi glukosa berbeda serta mengetahui isolat dengan konsentrasi glukosa optimum yang memiliki aktivitas enzim terbaik dalam pewarna Indigosol Blue. Pengukuran aktivitas enzimatik dilakukan dengan metode spektrofotometri. Hasil penelitian menunjukkan bahwa isolat-isolat uji dapat menghasilkan enzim dalam pewarna Indigosol Blue pada konsentrasi glukosa berbeda. Aktivitas enzim pada masing-masing perlakuan berbeda-beda dan menunjukkan hasil yang signifikan. Data uji lanjut memperlihatkan bahwa isolat Trametes sp. strain A memiliki aktivitas enzim terbaik dalam pewarna Indigosol Blue dengan konsentrasi glukosa 0,5%. Penelitian juga menunjukkan bahwa konsentrasi glukosa di atas 0.5% dapat menghambat aktivitas lakase, sehingga aktivitas lakase dalam zat pewarna rendah.
Kata kunci: enzim ligninolitik, fungi, glukosa, Indigosol Blue, Trametes sp.
References
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Zavarzina, A.G., & Zavarzin, A.A., 2006. Laccase and Tyrosinase Activities in Lichens. Microbiology, 75(5): 546-556.
Ado, B.V., Onilude, A.A., & Amande, T., 2018. Production and Optimization of Laccase by Trametes sp. Isolate B7 and Its’ Dye Decolourization Potential. Journal of Advanced Microbiology, 13(1): 1-14.
Campos, R., Kandelbauer, A., Robra, K.H., Cavaco-Paulo, A., & Gubitz, G.M., 2001. Indigo Degradation with Purified Laccases from Trametes hirsuta and Sclerotium rolfsii. Journal of Biotechnology, 89: 131-139.
Castillo, P.Z., 2012. Purification and Characterization of Laccase from Trametes hirsuta Bm-2 and Its Contribution to Dye and Effluent Decolorization. African Journal of Biotechnology, 11(15): 3603-3611.
Herfiani, Z.H., Rezagama, A., Nur, M., 2017. Pengolahan Limbah Cair Zat Warna Jenis Indigosol Blue (C.I Vat Blue 4) sebagai Hasil Produksi Kain Batik Menggunakan Metode Ozonasi dan Adsorpsi Arang Aktif Batok Kelapa terhadap Parameter COD dan Warna. Jurnal Teknik Lingkungan, 6(3): 1-10.
Kapdan, I.K., Kargi, F., McMullan, G., & Marchant, R., 2000. Effect of Environmental Conditions on Biological Decolorization of Textile Dyestuff by C. versicolor. Enzyme and Microbial Technology, 26(5): 381-387.
Kartikasari, T.H., Lestari, S., & Dewi, R.S., 2012. Adsorpsi Zn dan Dekolorisasi Limbah Batik Menggunakan Limbah Baglog Pleurotus ostreatus dengan Sistem Inkubasi dan Volume Limbah Batik Berbeda. Biosfera, 29(3): 168-174.
Kaushik, P., Malik, A., 2009. Fungal Dye Decolourization: Recent Advances and Future Potential. Environment International, 35: 127-141.
Maalej-Kammoun, M., Zouari-Mechichi, H., Belbahri, L., Woodward, S., & Mechichi, T., 2009. Malachite Green Decolourization and Detoxification by The Laccase from a Newly Isolated Strain of Trametes sp. International Biodeterioration & Biodegradation, 63(5): 600-606.
Mahfudloh & Lestari, H., 2017. Strategi Penanganan Limbah Industri Batik di Kota Pekalongan. Skripsi. Semarang: Universitas Diponogoro.
Martina, A., Roza, R.M., & Sirait, J.R., 2015. Biodegradasi Pewarna Azo Mordant Black 17 Oleh Ganoderma sp. BTA1 Isolat Lokal. Prosiding Semirata 2015 bidang MIPA BKS-PTN Barat. Universitas Tanjungpura Pontianak.
Mechichi, T., Mhiri, N., & Sayadi, S., 2006. Remazol Brilliant Blue R Decolourization by The Laccase from Trametes trogii. Chemosphere, 64(6): 998-1005.
Mishra, A., Kumar, S., & Bhatnagar, A., 2019. Potential of Fungal Laccase in Decolorization of Synthetic Dyes. Microbial Wastewater Treatment, 7: 127-151.
Munir, E., Rahayu V., Priyani, N., & Yurnaliza., 2018. Decolorization of Batik Naphthol Dye by Local Ligninolytic Fungal Isolates. Journal of Physics: Conference of Science and Technology, 1116(2018): 1-7.
Ngieng, N.S., Zulkharnain, A., Roslan, H.A., & Husaini, A., 2013. Decolourisation of Synthetic Dyes by Endophytic Fungal FloraIsolated from Senduduk Plant (Melastoma malabathricum). ISRN Biotechnology, 2013(7): 1-7.
Ottoni, C., Simoes, M.E., Fernandes, S., Santos, C.R., & Lima, N., 2016. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs. International Journal of Environmental Research and Public Health, 13(8): 778-787.
Salleh, M.A.M., Mahmoud, D.K., Karim, W.A., & Idris, A., 2011. Cationic and Anionic Dye Adsorption by Agricultural Solid Wastes: A Comprehensive Review. Desalination, 280(1): 1-13.
Sari, A.A., Tachibana, S., & Muryanto., 2012. Correlation of Ligninolytic Enzymes from the Newly-Found Species Trametes versicolor U97 with RBBR Decolorization and DDT Degradation. Water Air Soil Pollution, 223: 5781-5792.
Sari, A.A. & Dewi, R.S., 2019. The Study of Basidiomycota and Glomeromycota Biodiversity in Baturraden Botanical Garden, Indonesia. Report Research. Research Grant Programme, NEF.
Senthilkumar, S., Perumalsamy, M., & Prabhu, H.J., 2014. Decolourization potential of white-rot fungus Phanerochaete chrysosporium on synthetic dye bath effluent containing Amido black 10B. Journal of Saudi Chemical Society, 18: 845-853.
Sulistyaningtyas, A.R. & Suprihadi, A., 2017. Produksi Miselium Jamur Ling Zhi (Ganoderma lucidum) dalam Medium Air Kelapa Tua dan Tauge Extract Broth dengan Metode Kultur Terendam Teragitasi. Bioma, 19(1): 58-81.
Sumarko, H.G., Lestari, S., & Dewi, R.S., 2013. Dekolorisasi Limbah Cair Batik Menggunakan Limbah Baglog Pleurotus ostreatus Dengan Kombinasi Volume Dan Waktu Inkubasi Berbeda. Molekul, 8(2): 152-157.
Suprihatatin, H., 2014. Kandungan Organik Limbah Cair Industri Batik Jetis Sidoarjo dan Alternatif Pengolahannya. Riau: Pusat Penelitian Lingkungan Hidup Universitas Riau.
Turhan, K., Durukan, I., Ozturkcan, S.A., & Turgut, Z., 2012. Decolorization of Textile Basic Dye in Aqueous Solution by Ozone. Dyes Pigments, 92(3): 897-901.
Wang, N., Chu, Y., Wu, F., Zhao, Z., & Xu, X., 2017. Decolorization and Degradation of Congo Red by A Newly IsolatedWhite Rot Fungus, Ceriporia lacerata, from Decayed Mulberry Branches. International Biodeterioration & Biodegradation, 117(2017): 236-244.
Yesilada, O., Birhanli, E., & Geckil, H., 2018. Bioremediation and Decolorization of Textile Dyes by White Rot Fungi and Laccase Enzymes. In: Prasad R. (eds) Mycoremediation and Environmental Sustainability. India: Springer International Publishing.
Zahra. 2017. Studi Dekolorisasi Lindi Hitam dengan Teknik Ramah Lingkungan Menggunakan Jamur Trametes versicolor F200 dan Isolat Enzimnya yang Diimobilisasikan pada Natrium Alginat. Thesis. Depok: Universitas Indonesia.
Zapata-Castillo, P., Villalonga-Santana, L., Islas-Flores, I., Rivera-Mu~noz, G., Ancona-Escalante, W., & Solis-Pereira, S., 2015. Synergistic Action of Laccases from Trametes hirsuta Bm2 Improves Decolourization of Indigo Carmine. Applied Microbiology, 61: 252-258.
Zavarzina, A.G., & Zavarzin, A.A., 2006. Laccase and Tyrosinase Activities in Lichens. Microbiology, 75(5): 546-556.
Published
2020-07-25
How to Cite
ADRIR, Mutia Syarifah; DEWI, Ratna Stia; SARI, Ajeng Arum.
Aktivitas Enzimatik Isolat Trametes spp. dari Kebun Raya Baturraden dalam Pewarna Batik dengan Variasi Konsentrasi Indigosol Blue Glukosa.
BioEksakta : Jurnal Ilmiah Biologi Unsoed, [S.l.], v. 2, n. 2, p. 174 - 180, july 2020.
ISSN 2714-8564.
Available at: <http://jos.unsoed.ac.id/index.php/bioe/article/view/1810>. Date accessed: 19 apr. 2024.
doi: https://doi.org/10.20884/1.bioe.2020.2.2.1810.
Section
Articles
