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Stroke patients often get more than two kinds of drugs, thus increase the incidence of drug interactions. This study aimed to determine the incidence of drug interactions in hospitalized stroke patients in stroke unit Banyumas General Hospital based on the Drug Interaction Facts book. This study was a descriptive evaluative study using retrospective data and conducted in total sampling. Data were analyzed descriptively and presen ted in the quantitative and qualitative data form. The results showed that of 62 patients there were 26% of stroke patients with drug interactions. The most common drug interaction was one kind of drug interactions (74%). Types of drug interactions that often occurred in stroke patients were drug interaction between Angiotensin Converting Enzyme Inhibitor
(ACEI) with KCl (18.18%). The most common mechanisms of drug interaction was unknown (36%). The most common significance level was 4th level (50%), with the onset of drug interactions was delayed (57%) and in moderate severity (57%). The most common documentation of drug interactions was possible (50%).